cDNA Clone MPND

• Plasmid: pcDNA5D FRT/TO GFP MPND
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-MPND
• Genbank: NM_032868.4 GI:229577176 with silent changes g177a, a597g and a1209g.
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCAGCTCCGGAGCCGCTGTCCCCGGCGGGCGGTGCGGGCGAGGAGGCGCCGGAGGAGGACGAGGACGAAGCGGAGGCCGAGGACCCTGAGCGGCCGAATGCGGGAGCGGGCGGTGGACGCAGTGGCGGCGGCGGCAGTAGCGTCAGCGGAGGAGGCGGCGGCGGCGGGGCCGGAGCGGGGGGCTGCGGCGGGCCCGGGGGCGCGCTCACCAGGCGCGCGGTCACACTGCGGGTGCTCCTCAAAGACGCGCTGCTGGAGCCTGGCGCCGGGGTGCTGTCCATCTACTACCTGGGGAAGAAGTTCCTGGGCGACCTGCAGCCAGACGGAAGGATCATGTGGCAGGAGACCGGGCAGACCTTCAACTCACCCAGCGCCTGGGCCACCCACTGCAAGAAGCTGGTGAACCCTGCCAAGAAGTCGGGCTGTGGCTGGGCCTCTGTCAAGTACAAAGGCCAGAAACTGGACAAGTACAAGGCCACCTGGCTCCGGCTGCACCAGCTGCACACGCCTGCCACGGCTGCTGATGAGAGCCCAGCCAGTGAAGGGGAGGAGGAGGAGTTGCTGATGGAAGAGGAGGAGGAGGACGTTCTGGCGGGGGTCTCAGCAGAGGACAAGAGTCGGAGACCACTGGGGAAGAGCCCTTCAGAGCCTGCCCACCCGGAGGCCACAACCCCAGGGAAGCGGGTGGACAGCAAGATCCGGGTTCCGGTCCGCTACTGCATGCTGGGCAGCCGCGACTTGGCCAGGAACCCCCACACCCTGGTGGAAGTAACATCCTTTGCAGCCATCAACAAGTTCCAGCCGTTCAACGTGGCTGTTTCTAGCAACGTGCTGTTCCTGCTGGACTTCCACAGTCACCTGACACGGAGTGAGGTCGTGGGTTACCTGGGGGGCCGCTGGGACGTCAACAGCCAGATGCTGACGGTGCTCAGAGCCTTCCCTTGTCGGAGCCGGCTCGGGGACGCAGAGACTGCAGCTGCCATCGAAGAGGAGATCTACCAGAGCCTGTTCCTGCGGGGCCTGTCCCTGGTGGGCTGGTACCACAGCCACCCACACAGCCCGGCGCTGCCATCTCTGCAGGACATCGACGCACAGATGGACTACCAGCTGCGGCTGCAGGGCTCCAGCAATGGCTTCCAGCCCTGCCTCGCCCTGCTCTGCTCCCCTTACTATTCTGGCAACCCAGGCCCCGAGTCCAAGATCTCGCCTTTCTGGGTGATGCCTCCTCCCGAGATGCTGCTGGTGGAGTTCTACAAGGGTTCCCCTGACCTCGTGAGGCTCCAGGAACCCTGGAGCCAGGAGCACACCTACCTCGACAAGCTTAAGATCTCCTTGGCCAGCAGGACGCCCAAGGACCAGAGCCTGTGTCACGTCCTGGAACAGGTGTGCGGCGTCCTCAAGCAGGGGAGCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAAPEP
LSPAGGAGEEAPEEDEDEAEAEDPERPNAGAGGGRSGGGGSSVSGGGGGG
GAGAGGCGGPGGALTRRAVTLRVLLKDALLEPGAGVLSIYYLGKKFLGDL
QPDGRIMWQETGQTFNSPSAWATHCKKLVNPAKKSGCGWASVKYKGQKLD
KYKATWLRLHQLHTPATAADESPASEGEEEELLMEEEEEDVLAGVSAEDK
SRRPLGKSPSEPAHPEATTPGKRVDSKIRVPVRYCMLGSRDLARNPHTLV
EVTSFAAINKFQPFNVAVSSNVLFLLDFHSHLTRSEVVGYLGGRWDVNSQ
MLTVLRAFPCRSRLGDAETAAAIEEEIYQSLFLRGLSLVGWYHSHPHSPA
LPSLQDIDAQMDYQLRLQGSSNGFQPCLALLCSPYYSGNPGPESKISPFW
VMPPPEMLLVEFYKGSPDLVRLQEPWSQEHTYLDKLKISLASRTPKDQSL
CHVLEQVCGVLKQGS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49127
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-term GFP-tagged MPND in pcDNA5 FRT/TO, please.