cDNA Clone MYPT1

• Synonyms: PPP1R12A, M130, MBS
• Plasmid: pGEX6P-2 MYPT1 T419A T500A T524A T529A T671A T761A T892A
• Parent Plasmid: pGEX6P2
• Expressed: GST-C3-MYPT T419A T500A T524A T529A T671A T761A T892A
• Genbank: NM_001143885.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAACGCTGGATCGGCTCCGAGACGGACCTCGAGCCTCCGGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCTGCTTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAATGTGGACGGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTGGTAGAAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTACATGCAGCAGCTTCCTGTGGATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCACATGTAGGGGCTGTCAACAGTGAAGGAGATACACCTTTAGATATTGCGGAGGAGGAGGCAATGGAAGAGCTACTTCAAAATGAAGTTAATCGGCAAGGGGTTGATATAGAAGCAGCTCGAAAGGAAGAAGAACGGATCATGCTTAGAGATGCCAGGCAGTGGCTAAATAGTGGTCATATAAATGATGTCCGGCATGCAAAATCTGGAGGTACAGCACTTCACGTTGCAGCTGCTAAAGGCTATACGGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGACTATGATGGCTGGACACCTCTTCATGCTGCAGCTCATTGGGGTAAAGAAGAAGCATGTCGAATTTTAGTGGACAATCTGTGTGATATGGAGATGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATTTTAGGATATTTAGAAGAGTTGCAAAAGAAACAAAATCTGCTCCATAGTGAAAAACGGGACAAGAAATCTCCACTAATTGAATCAACAGCAAATATGGACAATAATCAGTCACAGAAGACCTTTAAAAACAAAGAGACGTTGATTATTGAACCAGAGAAAAATGCATCCCGTATTGAATCTCTGGAACAAGAAAAGGTTGATGAAGAAGAAGAAGGAAAGAAGGATGAGTCTAGCTGCTCTAGTGAAGAAGATGAGGAAGATGACTCGGAATCAGAAGCTGAAACAGATAAGACAAAACCCCTGGCTTCTGTAACTAATGCCAACACTTCTAGTACACAAGCAGCTCCTGTAGCTGTTACAACACCTACTGTGTCATCAGGTCAAGCAACACCTACATCACCTATTAAAAAGTTTCCAACCACAGCTGCAAAAATTTCTCCCAAAGAAGAAGAGAGAAAAGATGAGTCTCCTGCAACTTGGAGGTTAGGACTTAGAAAGACGGGCAGCTATGGTGCACTTGCTGAAATCACAGCATCTAAAGAGGGTCAGAAAGAAAAAGATACTGCAGGTGTTACACGTTCAGCTTCAAGTCCCAGACTTTCCTCCTCTTTGGATAATAAAGAAAAGGAGAAAGATAGTAAAGGAACTAGGCTTGCATATGTTGCACCTGCAATACCAAGACGACTAGCCAGTACATCTGACATTGAAGAGAAAGAAAACAGAGATTCTTCAAGTTTGCGAGCAAGTAGTTCATATGCAAGGAGAAAATGGGAAGATGATCTTAAAAAAAATAGCTCAGTTAATGAAGGATCAACGTATCATAAAAGTTGCTCCTTTGGTAGAAGACAAGATGATTTGATTAGTTCTAGTGTTCCAAGCACCACATCAACACCAACAGTTACCTCTGCAGCTGGGCTTCAGAAAAGCCTGCTTTCCAGCACAAGCACTACTACAAAGATTACAACGGGTTCTTCCTCAGCAGGCACACAAAGCAGTACCTCAAATCGTTTGTGGGCTGAGGATAGTACTGAGAAAGAAAAGGACAGTGTTCCTACGGCAGTGACCATTCCTGTTGCTCCAACTGTTGTAAATGCTGCAGCTTCTACCACAACCCTGACTACAACTACTGCTGGCACTGTCTCCTCCACAACAGAGGTCAGGGAGAGACGCAGATCATACCTCGCTCCTGTTAGGGATGAAGAGTCTGAATCCCAAAGAAAAGCAAGATCTAGACAAGCAAGACAATCTAGAAGATCAACACAGGGAGTGACATTAACTGATCTTCAAGAAGCTGAGAAAACAATAGGAAGAAGTCGTTCTACCCGAACCAGAGAACAAGAAAATGAAGAAAAAGAAAAAGAGGAAAAAGAGAAACAAGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAACATCTAGAGAAGATGAATATAAACAAAAGTACTCCAGAGCGTATGATGAGACTTACCAGCGTTATAGGCCAGTATCAACTTCAAGTTCAACCACTCCATCCTCTTCACTTTCTACTATGAGCAGTTCACTGTATGCTTCAAGTCAACTAAACAGGCCAAATAGTCTTGTAGGCATAACTTCTGCTTACTCCAGAGGAATAACAAAAGAAAATGAAAGAGAGGGAGAAAAAAGAGAAGAGGAGAAAGAAGGAGAAGATAAATCACAACCTAAATCAATCAGAGAACGACGACGACCAAGAGAGAAAAGAAGATCTACAGGAGTTTCATTTTGGACACAAGATAGTGATGAAAATGAACAAGAACAACAATCAGACACAGAAGAGGGATCCAATAAGAAAGAAACTCAGACGGATTCCATTTCTAGATATGAAGCCAGTTCTACATCAGCTGGTGATCGATATGATTCCTTGCTGGGTCGCTCTGGATCATACAGTTACTTAGAAGAAAGAAAACCTTACAGCAGCAGGCTAGAAAAGGATGACTCAACTGACTTTAAAAAGCTTTATGAACAAATTCTAGCTGAAAATGAAAAGCTGAAGGCACAGCTACATGATACAAATATGGAACTAACAGATCTTAAATTACAGTTGGAAAAGGCCACCCAGAGACAAGAAAGATTTGCTGATAGATCACTGTTGGAAATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGAAGAATATCTGAAATGGAAGAAGAGCTCAAAATGTTACCAGACCTAAAAGCAGACAACCAGAGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEIPGSTRAAAMKMADAKQ
KRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLK
LLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWI
PLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNE
VNRQGVDIEAARKEEERIMLRDARQWLNSGHINDVRHAKSGGTALHVAAA
KGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDM
EMVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKRDKKSPLIESTANM
DNNQSQKTFKNKETLIIEPEKNASRIESLEQEKVDEEEEGKKDESSCSSE
EDEEDDSESEAETDKTKPLASVTNANTSSTQAAPVAVTTPTVSSGQATPT
SPIKKFPTTAAKISPKEEERKDESPATWRLGLRKTGSYGALAEITASKEG
QKEKDTAGVTRSASSPRLSSSLDNKEKEKDSKGTRLAYVAPAIPRRLAST
SDIEEKENRDSSSLRASSSYARRKWEDDLKKNSSVNEGSTYHKSCSFGRR
QDDLISSSVPSTTSTPTVTSAAGLQKSLLSSTSTTTKITTGSSSAGTQSS
TSNRLWAEDSTEKEKDSVPTAVTIPVAPTVVNAAASTTTLTTTTAGTVSS
TTEVRERRRSYLAPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAE
KTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEEKKESETSREDEYKQKY
SRAYDETYQRYRPVSTSSSTTPSSSLSTMSSSLYASSQLNRPNSLVGITS
AYSRGITKENEREGEKREEEKEGEDKSQPKSIRERRRPREKRRSTGVSFW
TQDSDENEQEQQSDTEEGSNKKETQTDSISRYEASSTSAGDRYDSLLGRS
GSYSYLEERKPYSSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELT
DLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLK
ADNQRLKDENGALIRVISKLSK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU48124
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: T419A T500A T524A T529A T671A T761A T892A