cDNA Clone BRD4

• Synonyms: Bromodomain Containing 4, HUNK1
• Plasmid: pFastbacDual 6P1 BRD4
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST BRD4
• Genbank: NM_058243.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCatgtctgcggagagcggccctgggacgagattgagaaatctgcc
agtaatgggggatggactagaaacttcccaaatgtctacaacacaggccc
aggcccaaccccagccagccaacgcagccagcaccaaccccccgccccca
gagacctccaaccctaacaagcccaagaggcagaccaaccaactgcaata
cctgctcagagtggtgctcaagacactatggaaacaccagtttgcatggc
ctttccagcagcctgtggatgccgtcaagctgaacctccctgattactat
aagatcattaaaacgcctatggatatgggaacaataaagaagcgcttgga
aaacaactattactggaatgctcaggaatgtatccaggacttcaacacta
tgtttacaaattgttacatctacaacaagcctggagatgacatagtctta
atggcagaagctctggaaaagctcttcttgcaaaaaataaatgagctacc
cacagaagaaaccgagatcatgatagtccaggcaaaaggaagaggacgtg
ggaggaaagaaacagggacagcaaaacctggcgtttccacggtaccaaac
acaactcaagcatcgactcctccgcagacccagacccctcagccgaatcc
tcctcctgtgcaggccacgcctcaccccttccctgccgtcaccccggacc
tcatcgtccagacccctgtcatgacagtggtgcctccccagccactgcag
acgcccccgccagtgcccccccagccacaacccccacccgctccagctcc
ccagcccgtacagagccacccacccatcatcgcggccaccccacagcctg
tgaagacaaagaagggagtgaagaggaaagcagacaccaccacccccacc
accattgaccccattcacgagccaccctcgctgcccccggagcccaagac
caccaagctgggccagcggcgggagagcagccggcctgtgaaacctccaa
agaaggacgtgcccgactctcagcagcacccagcaccagagaagagcagc
aaggtctcggagcagctcaagtgctgcagcggcatcctcaaggagatgtt
tgccaagaagcacgccgcctacgcctggcccttctacaagcctgtggacg
tggaggcactgggcctacacgactactgtgacatcatcaagcaccccatg
gacatgagcacaatcaagtctaaactggaggcccgtgagtaccgtgatgc
tcaggagtttggtgctgacgtccgattgatgttctccaactgctataagt
acaaccctcctgaccatgaggtggtggccatggcccgcaagctccaggat
gtgttcgaaatgcgctttgccaagatgccggacgagcctgaggagccagt
ggtggccgtgtcctccccggcagtgccccctcccaccaaggttgtggccc
cgccctcatccagcgacagcagcagcgatagctcctcggacagtgacagt
tcgactgatgactctgaggaggagcgagcccagcggctggctgagctcca
ggagcagctcaaagccgtgcacgagcagcttgcagccctctctcagcccc
agcagaacaaaccaaagaaaaaggagaaagacaagaaggaaaagaaaaaa
gaaaagcacaaaaggaaagaggaagtggaagagaataaaaaaagcaaagc
caaggaacctcctcctaaaaagacgaagaaaaataatagcagcaacagca
atgtgagcaagaaggagccagcgcccatgaagagcaagccccctcccacg
tatgagtcggaggaagaggacaagtgcaagcctatgtcctatgaggagaa
gcggcagctcagcttggacatcaacaagctccccggcgagaagctgggcc
gcgtggtgcacatcatccagtcacgggagccctccctgaagaattccaac
cccgacgagattgaaatcgactttgagaccctgaagccgtccacactgcg
tgagctggagcgctatgtcacctcctgtttgcggaagaaaaggaaacctc
aagctgagaaagttgatgtgattgccggctcctccaagatgaagggcttc
tcgtcctcagagtcggagagctccagtgagtccagctcctctgacagcga
agactccgaaacagagatggctccgaagtcaaaaaagaaggggcaccccg
ggagggagcagaagaagcaccatcatcaccaccatcagcagatgcagcag
gccccggctcctgtgccccagcagccgcccccgcctccccagcagccccc
accgcctccacctccgcagcagcaacagcagccgccacccccgcctcccc
caccctccatgccgcagcaggcagccccggcgatgaagtcctcgccccca
cccttcattgccacccaggtgcccgtcctggagccccagctcccaggcag
cgtctttgaccccatcggccacttcacccagcccatcctgcacctgccgc
agcctgagctgccccctcacctgccccagccgcctgagcacagcactcca
ccccatctcaaccagcacgcagtggtctctcctccagctttgcacaacgc
actaccccagcagccatcacggcccagcaaccgagccgctgccctgcctc
ccaagcccgcccggcccccagccgtgtcaccagccttgacccaaacaccc
ctgctcccacagccccccatggcccaacccccccaagtgctgctggagga
tgaagagccacctgccccacccctcacctccatgcagatgcagctgtacc
tgcagcagctgcagaaggtgcagccccctacgccgctactcccttccgtg
aaggtgcagtcccagcccccaccccccctgccgcccccaccccacccctc
tgtgcagcagcagctgcagcagcagccgccaccacccccaccaccccagc
cccagcctccaccccagcagcagcatcagccccctccacggcccgtgcac
ttgcagcccatgcagttttccacccacatccaacagcccccgccacccca
gggccagcagcccccccatccgcccccaggccagcagccacccccgccgc
agcctgccaagcctcagcaagtcatccagcaccaccattcaccccggcac
cacaagtcggacccctactcaaccggtcacctccgcgaagccccctcccc
gcttatgatacattccccccagatgtcacagttccagagcctgacccacc
agtctccaccccagcaaaacgtccagcctaagaaacaggagctgcgtgct
gcctccgtggtccagccccagcccctcgtggtggtgaaggaggagaagat
ccactcacccatcatccgcagcgagcccttcagcccctcgctgcggccgg
agccccccaagcacccggagagcatcaaggcccccgtccacctgccccag
cggccggaaatgaagcctgtggatgtcgggaggcctgtgatccggccccc
agagcagaacgcaccgccaccaggggcccctgacaaggacaaacagaaac
aggagccgaagactccagttgcgcccaaaaaggacctgaaaatcaagaac
atgggctcctgggccagcctagtgcagaagcatccgaccaccccctcctc
cacagccaagtcatccagcgacagcttcgagcagttccgccgcgccgctc
gggagaaagaggagcgtgagaaggccctgaaggctcaggccgagcacgct
gagaaggagaaggagcggctgcggcaggagcgcatgaggagccgagagga
cgaggatgcgctggagcaggcccggcgggcccatgaggaggcacgtcggc
gccaggagcagcagcagcagcagcgccaggagcaacagcagcagcagcaa
cagcaagcagctgcggtggctgccgccgccaccccacaggcccagagctc
ccagccccagtccatgctggaccagcagagggagttggcccggaagcggg
agcaggagcgaagacgccgggaagccatggcagctaccattgacatgaat
ttccagagtgatctattgtcaatatttgaagaaaatcttttctgagcggc
cgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSAESGPGTRLRNLPVMGD
GLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRV
VLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYY
WNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEET
EIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQ
ATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQ
SHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLG
QRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKH
AAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFG
ADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVS
SPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLK
AVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPP
PKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLS
LDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELER
YVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSET
EMAPKSKKKGHPGREQKKHHHHHHQQMQQAPAPVPQQPPPPPQQPPPPPP
PQQQQQPPPPPPPPSMPQQAAPAMKSSPPPFIATQVPVLEPQLPGSVFDP
IGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQ
PSRPSNRAAALPPKPARPPAVSPALTQTPLLPQPPMAQPPQVLLEDEEPP
APPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQ
LQQQPPPPPPPQPQPPPQQQHQPPPRPVHLQPMQFSTHIQQPPPPQGQQP
PHPPPGQQPPPPQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIH
SPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPI
IRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNA
PPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKS
SSDSFEQFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDAL
EQARRAHEEARRRQEQQQQQRQEQQQQQQQQAAAVAAAATPQAQSSQPQS
MLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU46329
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: NOTE; Insert is very hard to sequence due to repeats - area between 2500-2850