cDNA Clone BRD4

• Plasmid: pRETROX Tight puro HA BRD4 V87I
• Parent Plasmid: pRetroX tight puro
• Expressed: HA BRD4 V87I
• Genbank: NM_058243.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTCTGCGGAGAGCGGCCCTGGGACGAGATTGAGAAATCTGCC
AGTAATGGGGGATGGACTAGAAACTTCCCAAATGTCTACAACACAGGCCC
AGGCCCAACCCCAGCCAGCCAACGCAGCCAGCACCAACCCCCCGCCCCCA
GAGACCTCCAACCCTAACAAGCCCAAGAGGCAGACCAACCAACTGCAATA
CCTGCTCAGAGTGGTGCTCAAGACACTATGGAAACACCAGTTTGCATGGC
CTTTCCAGCAGCCTaTcGATGCCGTCAAGCTGAACCTCCCTGATTACTAT
AAGATCATTAAAACGCCTATGGATATGGGAACAATAAAGAAGCGCTTGGA
AAACAACTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTA
TGTTTACAAATTGTTACATCTACAACAAGCCTGGAGATGACATAGTCTTA
ATGGCAGAAGCTCTGGAAAAGCTCTTCTTGCAAAAAATAAATGAGCTACC
CACAGAAGAAACCGAGATCATGATAGTCCAGGCAAAAGGAAGAGGACGTG
GGAGGAAAGAAACAGGGACAGCAAAACCTGGCGTTTCCACGGTACCAAAC
ACAACTCAAGCATCGACTCCTCCGCAGACCCAGACCCCTCAGCCGAATCC
TCCTCCTGTGCAGGCCACGCCTCACCCCTTCCCTGCCGTCACCCCGGACC
TCATCGTCCAGACCCCTGTCATGACAGTGGTGCCTCCCCAGCCACTGCAG
ACGCCCCCGCCAGTGCCCCCCCAGCCACAACCCCCACCCGCTCCAGCTCC
CCAGCCCGTACAGAGCCACCCACCCATCATCGCGGCCACCCCACAGCCTG
TGAAGACAAAGAAGGGAGTGAAGAGGAAAGCAGACACCACCACCCCCACC
ACCATTGACCCCATTCACGAGCCACCCTCGCTGCCCCCGGAGCCCAAGAC
CACCAAGCTGGGCCAGCGGCGGGAGAGCAGCCGGCCTGTGAAACCTCCAA
AGAAGGACGTGCCCGACTCTCAGCAGCACCCAGCACCAGAGAAGAGCAGC
AAGGTCTCGGAGCAGCTCAAGTGCTGCAGCGGCATCCTCAAGGAGATGTT
TGCCAAGAAGCACGCCGCCTACGCCTGGCCCTTCTACAAGCCTGTGGACG
TGGAGGCACTGGGCCTACACGACTACTGTGACATCATCAAGCACCCCATG
GACATGAGCACAATCAAGTCTAAACTGGAGGCCCGTGAGTACCGTGATGC
TCAGGAGTTTGGTGCTGACGTCCGATTGATGTTCTCCAACTGCTATAAGT
ACAACCCTCCTGACCATGAGGTGGTGGCCATGGCCCGCAAGCTCCAGGAT
GTGTTCGAAATGCGCTTTGCCAAGATGCCGGACGAGCCTGAGGAGCCAGT
GGTGGCCGTGTCCTCCCCGGCAGTGCCCCCTCCCACCAAGGTTGTGGCCC
CGCCCTCATCCAGCGACAGCAGCAGCGATAGCTCCTCGGACAGTGACAGT
TCGACTGATGACTCTGAGGAGGAGCGAGCCCAGCGGCTGGCTGAGCTCCA
GGAGCAGCTCAAAGCCGTGCACGAGCAGCTTGCAGCCCTCTCTCAGCCCC
AGCAGAACAAACCAAAGAAAAAGGAGAAAGACAAGAAGGAAAAGAAAAAA
GAAAAGCACAAAAGGAAAGAGGAAGTGGAAGAGAATAAAAAAAGCAAAGC
CAAGGAACCTCCTCCTAAAAAGACGAAGAAAAATAATAGCAGCAACAGCA
ATGTGAGCAAGAAGGAGCCAGCGCCCATGAAGAGCAAGCCCCCTCCCACG
TATGAGTCGGAGGAAGAGGACAAGTGCAAGCCTATGTCCTATGAGGAGAA
GCGGCAGCTCAGCTTGGACATCAACAAGCTCCCCGGCGAGAAGCTGGGCC
GCGTGGTGCACATCATCCAGTCACGGGAGCCCTCCCTGAAGAATTCCAAC
CCCGACGAGATTGAAATCGACTTTGAGACCCTGAAGCCGTCCACACTGCG
TGAGCTGGAGCGCTATGTCACCTCCTGTTTGCGGAAGAAAAGGAAACCTC
AAGCTGAGAAAGTTGATGTGATTGCCGGCTCCTCCAAGATGAAGGGCTTC
TCGTCCTCAGAGTCGGAGAGCTCCAGTGAGTCCAGCTCCTCTGACAGCGA
AGACTCCGAAACAGAGATGGCTCCGAAGTCAAAAAAGAAGGGGCACCCCG
GGAGGGAGCAGAAGAAGCACCATCATCACCACCATCAGCAGATGCAGCAG
GCCCCGGCTCCTGTGCCCCAGCAGCCGCCCCCGCCTCCCCAGCAGCCCCC
ACCGCCTCCACCTCCGCAGCAGCAACAGCAGCCGCCACCCCCGCCTCCCC
CACCCTCCATGCCGCAGCAGGCAGCCCCGGCGATGAAGTCCTCGCCCCCA
CCCTTCATTGCCACCCAGGTGCCCGTCCTGGAGCCCCAGCTCCCAGGCAG
CGTCTTTGACCCCATCGGCCACTTCACCCAGCCCATCCTGCACCTGCCGC
AGCCTGAGCTGCCCCCTCACCTGCCCCAGCCGCCTGAGCACAGCACTCCA
CCCCATCTCAACCAGCACGCAGTGGTCTCTCCTCCAGCTTTGCACAACGC
ACTACCCCAGCAGCCATCACGGCCCAGCAACCGAGCCGCTGCCCTGCCTC
CCAAGCCCGCCCGGCCCCCAGCCGTGTCACCAGCCTTGACCCAAACACCC
CTGCTCCCACAGCCCCCCATGGCCCAACCCCCCCAAGTGCTGCTGGAGGA
TGAAGAGCCACCTGCCCCACCCCTCACCTCCATGCAGATGCAGCTGTACC
TGCAGCAGCTGCAGAAGGTGCAGCCCCCTACGCCGCTACTCCCTTCCGTG
AAGGTGCAGTCCCAGCCCCCACCCCCCCTGCCGCCCCCACCCCACCCCTC
TGTGCAGCAGCAGCTGCAGCAGCAGCCGCCACCACCCCCACCACCCCAGC
CCCAGCCTCCACCCCAGCAGCAGCATCAGCCCCCTCCACGGCCCGTGCAC
TTGCAGCCCATGCAGTTTTCCACCCACATCCAACAGCCCCCGCCACCCCA
GGGCCAGCAGCCCCCCCATCCGCCCCCAGGCCAGCAGCCACCCCCGCCGC
AGCCTGCCAAGCCTCAGCAAGTCATCCAGCACCACCATTCACCCCGGCAC
CACAAGTCGGACCCCTACTCAACCGGTCACCTCCGCGAAGCCCCCTCCCC
GCTTATGATACATTCCCCCCAGATGTCACAGTTCCAGAGCCTGACCCACC
AGTCTCCACCCCAGCAAAACGTCCAGCCTAAGAAACAGGAGCTGCGTGCT
GCCTCCGTGGTCCAGCCCCAGCCCCTCGTGGTGGTGAAGGAGGAGAAGAT
CCACTCACCCATCATCCGCAGCGAGCCCTTCAGCCCCTCGCTGCGGCCGG
AGCCCCCCAAGCACCCGGAGAGCATCAAGGCCCCCGTCCACCTGCCCCAG
CGGCCGGAAATGAAGCCTGTGGATGTCGGGAGGCCTGTGATCCGGCCCCC
AGAGCAGAACGCACCGCCACCAGGGGCCCCTGACAAGGACAAACAGAAAC
AGGAGCCGAAGACTCCAGTTGCGCCCAAAAAGGACCTGAAAATCAAGAAC
ATGGGCTCCTGGGCCAGCCTAGTGCAGAAGCATCCGACCACCCCCTCCTC
CACAGCCAAGTCATCCAGCGACAGCTTCGAGCAGTTCCGCCGCGCCGCTC
GGGAGAAAGAGGAGCGTGAGAAGGCCCTGAAGGCTCAGGCCGAGCACGCT
GAGAAGGAGAAGGAGCGGCTGCGGCAGGAGCGCATGAGGAGCCGAGAGGA
CGAGGATGCGCTGGAGCAGGCCCGGCGGGCCCATGAGGAGGCACGTCGGC
GCCAGGAGCAGCAGCAGCAGCAGCGCCAGGAGCAACAGCAGCAGCAGCAA
CAGCAAGCAGCTGCGGTGGCTGCCGCCGCCACCCCACAGGCCCAGAGCTC
CCAGCCCCAGTCCATGCTGGACCAGCAGAGGGAGTTGGCCCGGAAGCGGG
AGCAGGAGCGAAGACGCCGGGAAGCCATGGCAGCTACCATTGACATGAAT
TTCCAGAGTGATCTATTGTCAATATTTGAAGAAAATCTTTTCTGAgcggc
cgc

Amino Acid Sequence

MYPYDVPDYAGSMSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPA
NAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPID
AVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYI
YNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGT
AKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPV
MTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGV
KRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDS
QQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLH
DYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHE
VVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDS
SSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKK
KEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEP
APMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQ
SREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDV
IAGSSKMKGFSSSESESSSESSSSDSEDSETEMAPKSKKKGHPGREQKKH
HHHHHQQMQQAPAPVPQQPPPPPQQPPPPPPPQQQQQPPPPPPPPSMPQQ
AAPAMKSSPPPFIATQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPH
LPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPP
AVSPALTQTPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKV
QPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQLQQQPPPPPPPQPQPPPQQ
QHQPPPRPVHLQPMQFSTHIQQPPPPQGQQPPHPPPGQQPPPPQPAKPQQ
VIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQN
VQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPE
SIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPPGAPDKDKQKQEPKTPV
APKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEQFRRAAREKEERE
KALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQ
QRQEQQQQQQQQAAAVAAAATPQAQSSQPQSMLDQQRELARKREQERRRR
EAMAATIDMNFQSDLLSIFEENLF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46377
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee