cDNA Clone BRD2

• Plasmid: pRetrox tight puro HA BRD2 V103I
• Parent Plasmid: pRetroX tight puro
• Expressed: HA BRD2 V103I
• Genbank: NM_001113182.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCTGCAAAACGTGACTCCCCACAATAAGCTCCCTGGGGAAGG
GAATGCAGGGTTGCTGGGGCTGGGCCCAGAAGCAGCAGCACCAGGGAAAA
GGATTCGAAAACCCTCTCTCTTGTATGAGGGCTTTGAGAGCCCCACAATG
GCTTCGGTGCCTGCTTTGCAACTTACCCCTGCCAACCCACCACCCCCGGA
GGTGTCCAATCCCAAAAAGCCAGGACGAGTTACCAACCAGCTGCAATACC
TACACAAGGTAGTGATGAAGGCTCTGTGGAAACATCAGTTCGCATGGCCA
TTCCGGCAGCCTaTtGATGCTGTCAAACTGGGTCTACCGGATTATCACAA
AATTATAAAACAGCCTATGGACATGGGTACTATTAAGAGGAGACTTGAAA
ACAATTATTATTGGGCTGCTTCAGAGTGTATGCAAGATTTTAATACCATG
TTCACCAACTGTTACATTTACAACAAGCCCACTGATGATATTGTCCTAAT
GGCACAAACGCTGGAAAAGATATTCCTACAGAAGGTTGCATCAATGCCAC
AAGAAGAACAAGAGCTGGTAGTGACCATCCCTAAGAACAGCCACAAGAAG
GGGGCCAAGTTGGCAGCGCTCCAGGGCAGTGTTACCAGTGCCCATCAGGT
GCCTGCCGTCTCTTCTGTGTCACACACAGCCCTGTATACTCCTCCACCTG
AGATACCTACCACTGTCCTCAACATTCCCCACCCATCAGTCATTTCCTCT
CCACTTCTCAAGTCCTTGCACTCTGCTGGACCCCCGCTCCTTGCTGTTAC
TGCAGCTCCTCCAGCCCAGCCCCTTGCCAAGAAAAAAGGCGTAAAGCGGA
AAGCAGATACTACCACCCCTACACCTACAGCCATCTTGGCTCCTGGTTCT
CCAGCTAGCCCTCCTGGGAGTCTTGAGCCTAAGGCAGCACGGCTTCCCCC
TATGCGTAGAGAGAGTGGTCGCCCCATCAAGCCCCCACGCAAAGACTTGC
CTGACTCTCAGCAACAACACCAGAGCTCTAAGAAAGGAAAGCTTTCAGAA
CAGTTAAAACATTGCAATGGCATTTTGAAGGAGTTACTCTCTAAGAAGCA
TGCTGCCTATGCTTGGCCTTTCTATAAACCAGTGGATGCTTCTGCACTTG
GCCTGCATGACTACCATGACATCATTAAGCACCCCATGGACCTCAGCACT
GTCAAGCGGAAGATGGAGAACCGTGATTACCGGGATGCACAGGAGTTTGC
TGCTGATGTACGGCTTATGTTCTCCAACTGCTATAAGTACAATCCCCCAG
ATCACGATGTTGTGGCAATGGCACGAAAGCTACAGGATGTATTTGAGTTC
CGTTATGCCAAGATGCCAGATGAACCACTAGAACCAGGGCCTTTACCAGT
CTCTACTGCCATGCCCCCTGGCTTGGCCAAATCGTCTTCAGAGTCCTCCA
GTGAGGAAAGTAGCAGTGAGAGCTCCTCTGAGGAAGAGGAGGAGGAAGAT
GAGGAGGACGAGGAGGAAGAAGAGAGTGAAAGCTCAGACTCAGAGGAAGA
AAGGGCTCATCGCTTAGCAGAACTACAGGAACAGCTTCGGGCAGTACATG
AACAACTGGCTGCTCTGTCCCAGGGTCCAATATCCAAGCCCAAGAGGAAA
AGAGAGAAAAAAGAGAAAAAGAAGAAACGGAAGGCAGAGAAGCATCGAGG
CCGAGCTGGGGCCGATGAAGATGACAAGGGGCCTAGGGCACCCCGCCCAC
CTCAACCTAAGAAGTCCAAGAAAGCAAGTGGCAGTGGGGGTGGCAGTGCT
GCTTTAGGCCCTTCCGGCTTTGGACCTTCTGGAGGAAGTGGCACCAAGCT
CCCCAAAAAGGCCACAAAGACAGCCCCACCTGCCCTGCCTACAGGTTATG
ATTCAGAGGAGGAGGAAGAGAGCAGGCCCATGAGTTACGATGAGAAGCGG
CAGCTGAGCCTGGACATCAACAAATTACCTGGGGAGAAGCTGGGCCGAGT
TGTGCATATAATCCAAGCCAGGGAGCCCTCTTTACGTGATTCAAACCCAG
AAGAGATTGAGATTGATTTTGAAACACTCAAGCCATCCACACTTAGAGAG
CTTGAGCGCTATGTCCTTTCCTGCCTACGTAAGAAACCCCGGAAGCCCTA
CACCATTAAGAAGCCTGTGGGAAAGACAAAGGAGGAACTGGCTTTGGAGA
AAAAGCGGGAATTAGAAAAGCGGTTACAAGATGTCAGCGGACAGCTCAAT
TCTACTAAAAAGCCCCCCAAGAAAGCGAATGAGAAAACAGAGTCATCCTC
TGCACAGCAAGTAGCAGTGTCACGCCTTAGCGCTTCCAGCTCCAGCTCAG
ATTCCAGCTCCTCCTCTTCCTCGTCGTCGTCTTCAGACACCAGTGATTCA
GACTCAGGCTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMLQNVTPHNKLPGEGNAGLLGLGPEAAAPGKRIRKPSL
LYEGFESPTMASVPALQLTPANPPPPEVSNPKKPGRVTNQLQYLHKVVMK
ALWKHQFAWPFRQPIDAVKLGLPDYHKIIKQPMDMGTIKRRLENNYYWAA
SECMQDFNTMFTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQELV
VTIPKNSHKKGAKLAALQGSVTSAHQVPAVSSVSHTALYTPPPEIPTTVL
NIPHPSVISSPLLKSLHSAGPPLLAVTAAPPAQPLAKKKGVKRKADTTTP
TPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPPRKDLPDSQQQH
QSSKKGKLSEQLKHCNGILKELLSKKHAAYAWPFYKPVDASALGLHDYHD
IIKHPMDLSTVKRKMENRDYRDAQEFAADVRLMFSNCYKYNPPDHDVVAM
ARKLQDVFEFRYAKMPDEPLEPGPLPVSTAMPPGLAKSSSESSSEESSSE
SSSEEEEEEDEEDEEEEESESSDSEEERAHRLAELQEQLRAVHEQLAALS
QGPISKPKRKREKKEKKKKRKAEKHRGRAGADEDDKGPRAPRPPQPKKSK
KASGSGGGSAALGPSGFGPSGGSGTKLPKKATKTAPPALPTGYDSEEEEE
SRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAREPSLRDSNPEEIEIDF
ETLKPSTLRELERYVLSCLRKKPRKPYTIKKPVGKTKEELALEKKRELEK
RLQDVSGQLNSTKKPPKKANEKTESSSAQQVAVSRLSASSSSSDSSSSSS
SSSSSDTSDSDSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46373
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: in pRETROX tight; ; as genbank except g93a (silent) t1809c (silent)