cDNA Clone Rpn10

• Synonyms: AF; AF-1; ASF; MCB1; pUB-R5; PSMD4; S5A
• Plasmid: pGEX6P Halo Thr Rpn10 M196-D309
• Parent Plasmid: pGEX6P Halo Thr
• Expressed: GST-C3-HALO-thr Rpn10 M196-D309
• Genbank: NM_002810.2 GI:78000204
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGCTGGGTCTTGGTGCCAGTGACTTTGAATTTGGAGTAGATCCCAGTGCTGATCCTGAGCTGGCCTTGGCCCTTCGTGTATCTATGGAAGAGCAGCGGCAGCGGCAGGAGGAGGAGGCCCGGCGGGCAGCTGCAGCTTCTGCTGCTGAGGCCGGGATTGCTACGACTGGGACTGAAGACTCAGACGATGCCCTGCTGAAGATGACCATCAGCCAGCAAGAGTTTGGCCGCACTGGGCTTCCTGACCTAAGCAGTATGACTGAGGAAGAGCAGATTGCTTATGCCATGCAGATGTCCCTGCAGGGAGCAGAGTTTGGCCAGGCGGAATCAGCAGACATTGATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMLGLGASDFEFGVD
PSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDA
LLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADID
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49625
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please clone the cDNA into pGEX C3 HALO thrombin vector. Thank you.