cDNA Clone FIP200

• Synonyms: CC1, FIP200, ATG17
• Plasmid: pcDNA5 FRT/TO GFP FIP200 S257A
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP FIP200 S257A
• Genbank: NM_014781.4 with changes: t701c (M234T), t4179c (silent), t4668c (silent)
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCGATGAAGTTATATGTATTTCTGGTTAACACTGGAACTACTCTAACATTTGACACTGAACTTACAGTGCAAACTGTGGCAGACCTTAAGCATGCCATTCAAAGCAAATACAAGATTGCTATTCAACACCAGGTGCTGGTGGTCAATGGAGGAGAATGCATGGCTGCAGATCGAAGAGTGTGTACCTACAGTGCTGGGACGGATACAAATCCAATTTTTCTTTTTAACAAAGAAATGATCTTATGTGATCGTCCACCTGCTATTCCTAAAACTACCTTTTCGACAGAAAATGACATGGAAATAAAAGTTGAAGAATCTCTTATGATGCCTGCAGTTTTTCATACTGTTGCTTCAAGGACACAGCTTGCATTGGAAATGTATGAAGTTGCCAAGAAACTTTGTTCTTTTTGTGAAGGTCTTGTACATGATGAACATCTTCAACACCAAGGCTGGGCTGCAATCATGGCCAACCTGGAGGACTGTTCAAATTCATACCAAAAGCTACTTTTCAAGTTTGAAAGTATTTATTCAAATTATCTGCAGTCCATAGAAGACATCAAGTTAAAACTTACTCATTTAGGAACTGCAGTTTCAGTAATGGCCAAGATTCCACTGTTGGAGTGCCTAACCAGACATAGTTACAGAGAATGTTTGGGAAGACTGGATTCTTTACCTGAACATGAAGACTCAGAAAAAGCTGAGACGAAAAGATCCACTGAACTGGTGCTCTCTCCTGATATGCCTAGAACAACTAACGAATCTTTGTTAACCGCCTTTCCCAAGTCAGTGGAACATGTGTCCCCAGATACCGCAGATGCTGAAAGTGGCAAAGAAATTAGGGAATCTTGTCAAAGTACTGTTCATCAGCAAGATGAAACTACGATTGACACTAAAGATGGTGATCTGCCCTTTTTTAATGTCTCTTTGTTAGACTGGATAAATGTTCAAGATAGACCTAATGATGTGGAATCTTTGGTCAGGAAGTGCTTTGATTCTATGAGCAGGCTTGATCCAAGGATTATTCGACCATTTATAGCAGAATGCCGTCAAACTATTGCCAAACTTGATAATCAGAATATGAAAGCCATTAAAGGACTTGAAGATCGGCTCTACGCCCTGGACCAGATGATTGCTAGCTGTGGCCGACTGGTGAATGAACAGAAAGAGCTTGCTCAGGGATTTTTAGCTAATCAGAAGAGAGCTGAAAACTTAAAGGATGCATCTGTATTACCTGATTTATGCCTGAGTCACGCAAATCAGTTGATGATTATGTTGCAAAATCATAGAAAACTGTTAGATATTAAGCAGAAGTGTACCACTGCCAAACAAGAACTAGCAAATAACCTACATGTCAGACTGAAGTGGTGTTGCTTTGTAATGCTTCATGCTGATCAAGATGGAGAGAAGTTACAAGCTTTGCTCCGCCTCGTAATAGAGCTGTTAGAAAGAGTCAAAATTGTTGAAGCTCTTAGTACAGTTCCTCAGATGTACTGCTTAGCTGTTGTTGAGGTTGTAAGAAGAAAAATGTTCATAAAACACTACAGGGAGTGGGCTGGTGCTTTAGTCAAAGATGGAAAGAGATTATATGAAGCAGAAAAATCAAAAAGGGAATCCTTTGGGAAATTATTTAGGAAGTCTTTTTTAAGAAATCGTCTGTTTAGGGGACTGGACTCCTGGCCCCCTTCCTTTTGTACTCAAAAGCCTCGAAAGTTTGACTGTGAACTTCCAGATATTTCATTAAAAGATTTACAGTTTCTGCAATCATTTTGTCCTTCGGAAGTTCAGCCATTCCTCAGGGTTCCCTTACTTTGTGACTTTGAACCTCTACACCAGCATGTACTTGCTCTACATAATTTGGTAAAAGCAGCACAAAGTTTGGATGAAATGTCACAGACCATTACAGATCTACTGAGTGAACAAAAGGCATCTGTGAGTCAGACATCCCCACAGTCTGCTTCTTCACCAAGGATGGAAAGTACAGCAGGAATTACAACTACTACCTCACCGAGAACTCCTCCACCACTGACTGTTCAGGATCCCTTATGTCCTGCAGTTTGTCCCTTAGAAGAATTATCTCCAGATAGTATTGATGCACATACGTTTGATTTTGAAACTATTCCCCATCCAAACATAGAACAGACTATTCACCAAGTTTCTTTAGACTTGGATTCATTAGCAGAAAGTCCTGAATCAGATTTTATGTCTGCTGTGAATGAGTTTGTAATAGAAGAAAATTTGTCGTCTCCTAATCCTATAAGTGATCCACAAAGCCCAGAAATGATGGTGGAATCACTTTATTCATCAGTTATCAATGCGATAGACAGTAGACGAATGCAGGATACAAATGTATGTGGTAAGGAGGATTTTGGAGATCATACTTCTCTGAATGTCCAGTTGGAAAGATGTAGAGTTGTTGCCCAAGACTCTCACTTCAGTATACAAACCATTAAGGAAGACCTTTGCCACTTTAGAACATTTGTACAAAAAGAACAGTGTGACTTCTCAAATTCATTAAAATGTACAGCAGTAGAAATAAGAAACATTATTGAAAAAGTAAAATGTTCTCTGGAAATAACACTAAAAGAAAAACATCAAAAAGAACTACTGTCTTTAAAAAATGAATATGAAGGTAAACTTGACGGACTAATAAAGGAAACTGAAGAGAATGAAAACAAAATTAAAAAATTGAAGGGAGAGTTAGTATGCCTTGAGGAGGTTTTACAAAATAAAGATAATGAATTTGCTTTGGTTAAACATGAAAAAGAAGCTGTAATCTGCCTGCAGAATGAAAAGGATCAGAAGTTGTTAGAGATGGAAAATATAATGCACTCTCAAAATTGTGAAATTAAAGAACTGAAGCAGTCACGAGAAATAGTGTTAGAAGACTTAAAAAAGCTCCATGTTGAAAATGATGAGAAGTTACAGTTATTGAGGGCAGAACTTCAGTCCTTGGAGCAAAGTCATCTAAAGGAATTAGAGGACACACTTCAGGTTAGGCACATACAAGAGTTTGAGAAGGTTATGACAGACCACAGAGTTTCTTTGGAGGAATTAAAAAAGGAAAACCAACAAATAATTAATCAAATACAAGAATCTCATGCTGAAATTATCCAGGAAAAAGAAAAACAGTTACAGGAATTAAAACTCAAGGTTTCTGATTTGTCAGACACGAGATGCAAGTTAGAGGTTGAACTTGCGTTGAAGGAAGCAGAAACTGATGAAATAAAAATTTTGCTGGAAGAAAGCAGAGCCCAGCAGAAGGAGACCTTGAAATCTCTTCTTGAACAAGAGACAGAAAATTTGAGAACAGAAATTAGTAAACTCAACCAAAAGATTCAGGATAATAATGAAAATTATCAGGTGGGCTTAGCAGAGCTAAGAACTTTAATGACAATTGAAAAAGATCAGTGTATTTCCGAGTTAATTAGTAGACATGAAGAAGAATCTAATATACTTAAAGCTGAATTAAACAAAGTAACATCTTTGCATAACCAAGCATTTGAAATAGAAAAAAACCTAAAAGAACAAATAATTGAACTGCAGAGTAAATTGGATTCAGAATTGAGTGCTCTTGAAAGACAAAAAGATGAAAAAATTACCCAACAAGAAGAGAAATACGAAGCTATTATCCAGAACCTTGAGAAAGACAGACAAAAATTGGTCAGCAGCCAGGAGCAAGACAGAGAACAGTTAATTCAGAAGCTTAATTGTGAAAAAGATGAAGCTATTCAGACTGCCCTAAAAGAATTTAAATTGGAGAGAGAAGTTGTTGAGAAAGAGTTATTAGAAAAAGTTAAACATCTTGAGAATCAAATAGCAAAAAGTCCTGCCATTGACTCTACCAGAGGAGATTCTTCAAGCTTAGTTGCTGAACTTCAAGAAAAGCTTCAGGAAGAAAAAGCTAAGTTTCTAGAACAACTTGAAGAGCAAGAAAAAAGAAAGAATGAAGAAATGCAAAATGTTCGAACATCTTTGATTGCGGAACAACAGACCAATTTTAACACTGTTTTAACAAGAGAGAAAATGAGAAAAGAAAACATAATAAATGATCTTAGTGATAAGTTGAAAAGTACAATGCAGCAACAAGAACGGGATAAAGATTTGATAGAGTCACTTTCTGAAGATCGAGCTCGTTTGCTTGAGGAAAAGAAAAAGCTTGAAGAAGAAGTCAGTAAGTTGCGCAGTAGCAGTTTTGTTCCTTCACCATATGTAGCTACAGCCCCAGAACTTTATGGAGCTTGTGCACCTGAACTCCCAGGTGAATCAGATAGATCCGCTGTGGAAACAGCAGATGAAGGAAGAGTGGATTCAGCAATGGAGACAAGCATGATGTCTGTACAAGAAAATATTCATATGTTGTCTGAAGAAAAACAGCGGATAATGCTGTTAGAACGAACATTGCAATTGAAAGAAGAAGAAAATAAACGGTTAAATCAAAGACTGATGTCTCAGAGCATGTCTTCAGTATCTTCAAGGCATTCTGAAAAGATAGCTATTAGAGATTTTCAGGTGGGAGATTTGGTACTCATCATCCTAGACGAACGCCATGACAATTATGTGTTATTTACTGTTAGTCCTACTTTATATTTTCTACATTCAGAGTCTCTACCTGCCCTGGATCTCAAACCAGGTGAGGGTGCTTCAGGTGCATCTAGAAGACCCTGGGTACTCGGAAAAGTAATGGAAAAAGAATACTGTCAAGCCAAAAAGGCACAAAACAGATTTAAAGTTCCTTTGGGGACAAAGTTTTACAGAGTGAAAGCCGTATCATGGAATAAGAAAGTATAACGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTI
DAAAMKLYVFLVNTGTTLTFDTELTVQTVADLKHAIQSKYKIAIQHQVLV
VNGGECMAADRRVCTYSAGTDTNPIFLFNKEMILCDRPPAIPKTTFSTEN
DMEIKVEESLMMPAVFHTVASRTQLALEMYEVAKKLCSFCEGLVHDEHLQ
HQGWAAIMANLEDCSNSYQKLLFKFESIYSNYLQSIEDIKLKLTHLGTAV
SVMAKIPLLECLTRHSYRECLGRLDSLPEHEDSEKAETKRSTELVLSPDM
PRTTNESLLTAFPKSVEHVSPDTADAESGKEIRESCQSTVHQQDETTIDT
KDGDLPFFNVSLLDWINVQDRPNDVESLVRKCFDSMSRLDPRIIRPFIAE
CRQTIAKLDNQNMKAIKGLEDRLYALDQMIASCGRLVNEQKELAQGFLAN
QKRAENLKDASVLPDLCLSHANQLMIMLQNHRKLLDIKQKCTTAKQELAN
NLHVRLKWCCFVMLHADQDGEKLQALLRLVIELLERVKIVEALSTVPQMY
CLAVVEVVRRKMFIKHYREWAGALVKDGKRLYEAEKSKRESFGKLFRKSF
LRNRLFRGLDSWPPSFCTQKPRKFDCELPDISLKDLQFLQSFCPSEVQPF
LRVPLLCDFEPLHQHVLALHNLVKAAQSLDEMSQTITDLLSEQKASVSQT
SPQSASSPRMESTAGITTTTSPRTPPPLTVQDPLCPAVCPLEELSPDSID
AHTFDFETIPHPNIEQTIHQVSLDLDSLAESPESDFMSAVNEFVIEENLS
SPNPISDPQSPEMMVESLYSSVINAIDSRRMQDTNVCGKEDFGDHTSLNV
QLERCRVVAQDSHFSIQTIKEDLCHFRTFVQKEQCDFSNSLKCTAVEIRN
IIEKVKCSLEITLKEKHQKELLSLKNEYEGKLDGLIKETEENENKIKKLK
GELVCLEEVLQNKDNEFALVKHEKEAVICLQNEKDQKLLEMENIMHSQNC
EIKELKQSREIVLEDLKKLHVENDEKLQLLRAELQSLEQSHLKELEDTLQ
VRHIQEFEKVMTDHRVSLEELKKENQQIINQIQESHAEIIQEKEKQLQEL
KLKVSDLSDTRCKLEVELALKEAETDEIKILLEESRAQQKETLKSLLEQE
TENLRTEISKLNQKIQDNNENYQVGLAELRTLMTIEKDQCISELISRHEE
ESNILKAELNKVTSLHNQAFEIEKNLKEQIIELQSKLDSELSALERQKDE
KITQQEEKYEAIIQNLEKDRQKLVSSQEQDREQLIQKLNCEKDEAIQTAL
KEFKLEREVVEKELLEKVKHLENQIAKSPAIDSTRGDSSSLVAELQEKLQ
EEKAKFLEQLEEQEKRKNEEMQNVRTSLIAEQQTNFNTVLTREKMRKENI
INDLSDKLKSTMQQQERDKDLIESLSEDRARLLEEKKKLEEEVSKLRSSS
FVPSPYVATAPELYGACAPELPGESDRSAVETADEGRVDSAMETSMMSVQ
ENIHMLSEEKQRIMLLERTLQLKEEENKRLNQRLMSQSMSSVSSRHSEKI
AIRDFQVGDLVLIILDERHDNYVLFTVSPTLYFLHSESLPALDLKPGEGA
SGASRRPWVLGKVMEKEYCQAKKAQNRFKVPLGTKFYRVKAVSWNKKV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49627
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Best at 30 degrees