cDNA Clone CULLIN1

• Synonyms: CUL1
• Plasmid: pMA CUL1 Nter FLAG donor
• Parent Plasmid: pMA
• Genbank: NM_003592.2
• Tag: FLAG
• Species: Human

Sequence of Insert

TGCCCTTTTTTCTTTGAGTTTGTCTTTCTAATTGTCATCTAGGTCAAGTGATATATATTTTTGATAACTTCCGAAGGCAAAGGAAGGCAACATAGTTGTGCTTGTGCAAACTTTTACCCAGGTTTTCTCTTGAGTGCTGATATCATAATGATTTTGAACTAAAATTCTAAGCGTATTTTTGTACACAGACCATATGGTTAACATGAATGTGTTTGCACATAGAAAGAGAAATGGTCTCTAGAGTGGGCCCTGCAGACTGAGTTTTTCTGTCAGTGTGTCTCACCTTATTACAGTACCCCAGAGACAATCCACTGATTCTCTTTATTTCTTCCTCAGGTTTGCCTGCAATGAGATTTCATTCTCTACATTTAAAGGACATCCTTTCTGAGCTGCTGTGAATAAATTTGGAATGGTACTGTATATTTTCATCTAATGGAGAACTAGCTGTACTTTGAATAAGGATTGCTGCACTGGACGACTTTAGAACATCCCTCACAATGaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgccGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCAGACTACAAGGACGATGACGATAAGTCGTCAACCCGGAGCCAGAACCCCCACGGCCTGAAGCAaATcGGtttaGAtCAaATaTGGGACGACCTCAGAGCCGGCATCCAGCAGGTGTACACACGGCAGAGCATGGCCAAGTCCAGATATATGGAGCTCTACACGTATCCTCCTGCCTAGCGCAGGTTGATTGCTTAGAGTTTTGATTATTTACTAGTATGACTTATGAATTATTAGAATTTTATATTGTTTTCTCCTCCCAGTTCATCATGTAAAATAATCGCAGGGTTCATTTTTAGCCTTAAGTTTTTGAACAGAGTAGAACTGGTTCATTTTTAGTCAAATGCAGCACTGGGCAGTACAGTCACCACTGGGCACATGTGGCTGTTTACATTTGAAATATTTTAAAGTTCCTTAGGCACACTAGCTGTGTGATTAATAAATAAAACATTTCTGTCATGTAGAAGGTTCCGTTGGGCAAAGCTGGTCTAAAGCAAGGTCCTGAGGTTCTCAGCAGATCATTTGTTTTACGTCCATTTGTTTATATATTGTCTATGACTGCCTTGTGTTATGGGAACAGAGTT

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48351
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame Puro-2A-FLAG into the first common coding exon (exon 2 isoform 1). Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Successful recombination will n-terminally tag CUL1 with FLAG, to be used in conjunction with DU48275.