cDNA Clone FANCL (chicken)

• Plasmid: pET15b 6HIS SUMO FANCL L7A D78R L79A I182V (chicken)
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6HIS SUMO FANCL L7A D78R L79A I182V (chicken)
• Genbank: NM_001033941.1
• Tag: 6His
• Tag: SUMO
• Species: Chicken

Sequence of Insert

ATGGCGGGTCGGGGATCTGCACTCCGCCAGTGTCCGCTGCTGCTGCCTCAGAACCGCGAGGGGACGGCCTATGAGGGCTTCGTCAGCGCGCAGGGTAGAGACTTCCACATCCGGATACTGTTACCAACAGATTCTCAACTGAAAAATGCAAGGATAGAGTGTAGCTGGCACCTAAAGAAGATACTGCATGGATATCAGCATATTTTGAAACAGAGGTTGCACAGTTGTCCAAGGGCAGTCAGTTTTGTGGTGGAGCTGAAAACTATTTTGGAAATTGCTTTGAAGAATACACAAGAGCTGCATGTACCGTGGCCACCAGAATACTACTCCTGTCTCATCAGAGATCTAGAAATATTGGGTTGGAATAAAGTTGCTTATGTGGATACTGGTCTCACCACAGTCAAGCTAAAAGCTGAAGACTCTCGTGGTCGACAACATCTGATCACTCTGAAGTTAAATGCAAAGTATCCTACTGAACCACCAGACTGCGTTGTGGATTTTCCTGTTCAGTTTGCCATTTCTTGGATGCCACAGGACTCATTAGTAGACATCCATAATCAGTTCTTGGCAGCATTAGAATCGCTGAAGGAGTTCTGGGACACCATGGATGAAATTGATGGGAAGACGTGGGTACTGGAGCCAGAAAATCCCGCAAGGTGTGCTACTTCAAGAAGGATCGCCATAGGGAACAACGTTTCCGTGAACATAGAGGTAGATCCCAGGCATCCAAAGATGCTCCCTGAGTGTTACTTCCTTGGAGCAGATCATGCGGTTAACCCTTTGAGAACTAAGCTGAACAATAACATGCACTTGTGGGATCCAGAAATTAGTTTATTACAAAACTTGAAAGACCTCTTAGAAATTGATTTTCCATCTCGTGCTGCATTAGAAAAAAGTGACTTTACTAAGGACTGTGGAATCTGCTATGCCTACCGCCTCAATGGCACCACGCCAGATCAAGTGTGCAATGAGCCCCGATGCGGACAACCTTTTCATCAGGCTTGCCTGTATGAGTGGCTACAAGGTCTTCCTTCCAGCAGACAGAGCTTTAATGTCATCTTTGGTGAATGTCCATATTGCAACAAGCCACTGACAGTGAAATCTTCCATGAAGAAACCTTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMAGRGSALRQCPLLLPQNREGTAYEGFVSAQGRDFHIRILLPTDSQLKNARIECSWHLKKILHGYQHILKQRLHSCPRAVSFVVELKTILEIALKNTQELHVPWPPEYYSCLIRDLEILGWNKVAYVDTGLTTVKLKAEDSRGRQHLITLKLNAKYPTEPPDCVVDFPVQFAISWMPQDSLVDIHNQFLAALESLKEFWDTMDEIDGKTWVLEPENPARCATSRRIAIGNNVSVNIEVDPRHPKMLPECYFLGADHAVNPLRTKLNNNMHLWDPEISLLQNLKDLLEIDFPSRAALEKSDFTKDCGICYAYRLNGTTPDQVCNEPRCGQPFHQACLYEWLQGLPSSRQSFNVIFGECPYCNKPLTVKSSMKKP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU25683
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: linker after SENP cleavage deleted. Has I182V SNP change but it is a tolerated change. Silent mutation A191