cDNA Clone CUL3 delta 403-459

• Plasmid: pcDNA5 FRT/TO FLag Cullin Delta 403-459 K712R
• Parent Plasmid: pcDNA5 FRT/TO FLAG
• Expressed: Flag CUL3 delta 403-459 K721R
• Genbank: NP_003581.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGTCGAATCTGAGCAAAGGCACGGGCAGCCGGAAGGACACCAAGATGCGTATCCGGGCCTTTCCGATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGATGTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATACTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTACGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCAATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATGTCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAATGAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCACATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTATTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAAGGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAATGACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGCTGAAAAAGGGAGTCAAAGGGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACAACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATATTGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAACACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGATCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTAATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGAAATTCAGCAAGAGACAGATATCCCTGAAAGAGAGCTTGTTAGAGCCCTACAGTCCCTCGCCTGTGGTAAACCAACACAGCGGGTTCTTACAAAAGAACCCAAATCAAAGGAAATAGAAAATGGTCATATATTTACAGTTAATGATCAATTCACATCCAAACTACACAGAGTCAAGATTCAAACAGTTGCTGCCAAACAAGGTGAATCCGACCCAGAGAGGAAAGAAACAAGGCAGAAAGTAGACGACGACAGAAAACATGAGATAGAAGCTGCTATAGTGCGGATAATGAGATCTAGAAAGAAGATGCAGCACAATGTTCTAGTAGCGGAGGTAACTCAGCAGTTGAAGGCGCGATTCTTACCAAGTCCAGTTGTTATTAAGAAACGTATTGAAGGACTTATTGAGAGAGAATATTTGGCACGAACACCTGAGGATCGCAAAGTATACACATATGTAGCATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMRSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25772
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Exon 9 Deleted. Mutant K712R is full length numbering and in this clone the mutation sits at K655R