cDNA Clone SMC2

• Synonyms: CAP-E; CAPE; SMC-2; SMC2L1
• Plasmid: pcDNA5 FRT/TO HA SMC2
• Parent Plasmid: pcDNA5 FRT/TO HA
• Expressed: HA SMC2
• Genbank: NM_006444.2 GI:110347417 with silent changes t2424g and g3222a
• Tag: HA
• Species: Human

Sequence of Insert

AGATCTATGCATATTAAGTCAATTATTCTAGAGGGATTCAAGTCCTATGCTCAGAGGACCGAAGTCAATGGTTTTGACCCCCTCTTCAATGCTATCACTGGCTTAAATGGTAGTGGGAAATCCAACATATTGGACTCCATCTGCTTTTTGCTGGGCATCTCCAACCTGTCTCAGGTTCGGGCTTCTAATTTACAAGATTTAGTTTACAAAAATGGGCAGGCTGGTATTACCAAAGCCTCTGTGTCAATCACTTTTGATAATTCTGACAAAAAGCAAAGTCCTTTAGGATTTGAGGTTCATGATGAAATCACAGTAACAAGGCAGGTGGTTATTGGTGGTAGAAATAAATATTTAATCAATGGAGTCAATGCCAACAACACCAGAGTACAGGATCTCTTCTGTTCTGTTGGCCTTAATGTTAACAACCCTCACTTTCTCATCATGCAGGGCCGAATTACAAAAGTATTGAATATGAAACCTCCAGAGATTTTATCCATGATAGAAGAAGCAGCTGGAACCAGGATGTATGAATACAAAAAAATAGCTGCACAGAAAACTATAGAAAAAAAGGAGGCTAAGCTGAAAGAAATTAAGACGATACTTGAAGAAGAGATTACTCCAACCATTCAAAAATTAAAAGAGGAAAGATCGTCCTACTTGGAGTACCAAAAAGTAATGAGAGAAATAGAACATTTGAGTCGTTTATATATTGCTTATCAGTTTTTGCTGGCTGAAGATACCAAAGTACGCTCAGCTGAGGAATTAAAAGAAATGCAAGATAAAGTTATAAAGCTTCAGGAAGAATTGTCTGAGAATGATAAAAAAATAAAAGCACTTAATCATGAAATAGAAGAATTGGAAAAAAGAAAAGATAAGGAAACTGGAGGTATACTTCGATCTTTAGAAGATGCTCTTGCAGAGGCTCAGCGAGTTAATACTAAATCTCAAAGCGCATTTGATCTCAAGAAGAAAAATCTGGCATGTGAGGAAAGCAAACGCAAAGAGCTGGAAAAAAATATGGTTGAGGACTCAAAAACTTTAGCAGCAAAGGAAAAAGAGGTTAAAAAGATAACAGATGGACTGCATGCCCTTCAAGAAGCAAGTAATAAAGATGCTGAAGCTCTGGCAGCTGCACAGCAGCACTTCAATGCTGTTTCCGCTGGCCTGTCCAGTAATGAAGATGGAGCAGAAGCAACTCTTGCTGGTCAAATGATGGCCTGTAAAAATGATATAAGTAAAGCTCAGACAGAAGCCAAACAGGCTCAGATGAAGTTGAAGCATGCTCAACAGGAATTAAAGAATAAACAAGCTGAAGTTAAGAAGATGGATAGTGGCTACAGGAAGGATCAAGAAGCTCTAGAAGCTGTAAAAAGACTTAAAGAAAAACTTGAAGCTGAAATGAAAAAGCTAAATTATGAAGAAAATAAAGAGGAAAGCCTTTTGGAAAAGCGCAGGCAGCTGTCTCGTGATATTGGTAGATTGAAAGAAACATATGAAGCTCTATTAGCCAGATTTCCCAATCTTCGATTTGCATACAAGGATCCAGAGAAGAACTGGAATAGAAATTGTGTGAAAGGACTTGTGGCTTCTCTGATTAGTGTGAAAGACACTTCTGCAACCACAGCTTTAGAATTAGTGGCTGGAGAACGACTCTACAATGTTGTAGTAGACACAGAAGTTACTGGTAAAAAGCTACTAGAAAGGGGGGAACTGAAACGTCGATACACTATAATTCCACTCAATAAAATTTCAGCCAGATGTATTGCACCAGAAACTCTGAGAGTTGCTCAGAATCTTGTTGGCCCTGACAACGTTCATGTGGCTCTTTCCTTGGTTGAATATAAACCAGAACTTCAGAAAGCAATGGAGTTTGTCTTTGGAACAACATTTGTTTGTGACAATATGGATAATGCCAAAAAAGTGGCCTTTGATAAGAGGATAATGACTAGAACTGTAACTCTCGGAGGTGATGTGTTTGATCCTCATGGGACATTGAGTGGAGGTGCTCGATCCCAGGCAGCTTCCATTTTAACCAAGTTTCAAGAACTCAAAGATGTTCAGGATGAACTGAGAATCAAAGAGAATGAGCTGCGGGCTCTAGAAGAGGAATTAGCAGGTCTTAAAAACACTGCTGAAAAGTATCGCCAACTAAAACAGCAGTGGGAGATGAAAACTGAAGAGGCAGATTTATTACAAACCAAGCTCCAGCAAAGCTCATATCACAAGCAACAAGAAGAATTAGATGCCCTTAAAAAAACCATTGAGGAAAGTGAGGAGACTTTGAAAAACACTAAAGAAATCCAAAGAAAAGCAGAAGAAAAATATGAAGTATTGGAAAATAAAATGAAAAATGCAGAAGCTGAAAGAGAGCGAGAACTGAAAGATGCTCAGAAAAAACTGGATTGTGCCAAAACAAAGGCAGATGCATCGAGCAAGAAGATGAAAGAAAAACAACAGGAAGTTGAAGCTATCACTCTGGAACTGGAAGAGCTCAAGAGAGAGCATACATCTTACAAACAACAGCTTGAAGCTGTAAATGAAGCTATCAAATCCTATGAAAGTCAGATTGAAGTAATGGCAGCTGAGGTGGCTAAAAATAAGGAGTCAGTAAATAAAGCTCAAGAAGAGGTGACCAAGCAAAAAGAGGTGATAACAGCCCAAGACACTGTAATTAAAGCTAAATATGCAGAAGTGGCAAAACACAAGGAGCAAAACAATGATTCTCAGCTTAAAATTAAGGAATTAGACCACAACATCAGCAAACATAAACGGGAGGCTGAAGATGGTGCTGCAAAGGTATCCAAAATGTTGAAAGATTATGACTGGATTAATGCAGAGAGACACCTCTTTGGCCAACCCAATAGTGCCTATGATTTCAAAACTAACAACCCTAAAGAAGCTGGTCAGAGACTTCAGAAGTTGCAAGAAATGAAGGAGAAACTAGGAAGAAATGTCAATATGAGAGCTATGAATGTATTGACAGAAGCTGAAGAGCGATACAATGACTTGATGAAGAAGAAGAGAATTGTAGAAAATGACAAATCCAAAATTCTTACAACTATAGAAGACCTTGACCAGAAGAAAAACCAAGCCCTAAATATTGCATGGCAAAAGGTGAACAAGGACTTTGGGTCTATTTTTTCTACTCTTTTGCCTGGTGCTAATGCTATGCTTGCACCACCAGAGGGTCAAACTGTTTTGGATGGTCTGGAGTTCAAGGTTGCCTTAGGAAATACCTGGAAAGAAAACCTAACTGAACTTAGTGGTGGTCAGAGGTCTTTAGTGGCCTTGTCATTAATACTGTCCATGCTTCTCTTCAAACCTGCTCCAATTTATATCCTTGATGAGGTAGATGCAGCCTTGGATCTTTCTCATACCCAAAACATTGGACAGATGCTGCGTACTCATTTCACACATTCTCAGTTCATTGTGGTGTCACTAAAAGAAGGTATGTTCAACAATGCAAACGTTCTTTTCAAAACCAAGTTTGTGGATGGTGTTTCTACAGTAGCCAGATTTACTCAATGTCAAAATGGAAAGATTTCAAAGGAAGCAAAATCCAAGGCAAAACCACCCAAAGGAGCACATGTGGAAGTTTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMHIKSIILEGFKSYAQRTEVNGFDPLFNAITGLNGSGK
SNILDSICFLLGISNLSQVRASNLQDLVYKNGQAGITKASVSITFDNSDK
KQSPLGFEVHDEITVTRQVVIGGRNKYLINGVNANNTRVQDLFCSVGLNV
NNPHFLIMQGRITKVLNMKPPEILSMIEEAAGTRMYEYKKIAAQKTIEKK
EAKLKEIKTILEEEITPTIQKLKEERSSYLEYQKVMREIEHLSRLYIAYQ
FLLAEDTKVRSAEELKEMQDKVIKLQEELSENDKKIKALNHEIEELEKRK
DKETGGILRSLEDALAEAQRVNTKSQSAFDLKKKNLACEESKRKELEKNM
VEDSKTLAAKEKEVKKITDGLHALQEASNKDAEALAAAQQHFNAVSAGLS
SNEDGAEATLAGQMMACKNDISKAQTEAKQAQMKLKHAQQELKNKQAEVK
KMDSGYRKDQEALEAVKRLKEKLEAEMKKLNYEENKEESLLEKRRQLSRD
IGRLKETYEALLARFPNLRFAYKDPEKNWNRNCVKGLVASLISVKDTSAT
TALELVAGERLYNVVVDTEVTGKKLLERGELKRRYTIIPLNKISARCIAP
ETLRVAQNLVGPDNVHVALSLVEYKPELQKAMEFVFGTTFVCDNMDNAKK
VAFDKRIMTRTVTLGGDVFDPHGTLSGGARSQAASILTKFQELKDVQDEL
RIKENELRALEEELAGLKNTAEKYRQLKQQWEMKTEEADLLQTKLQQSSY
HKQQEELDALKKTIEESEETLKNTKEIQRKAEEKYEVLENKMKNAEAERE
RELKDAQKKLDCAKTKADASSKKMKEKQQEVEAITLELEELKREHTSYKQ
QLEAVNEAIKSYESQIEVMAAEVAKNKESVNKAQEEVTKQKEVITAQDTV
IKAKYAEVAKHKEQNNDSQLKIKELDHNISKHKREAEDGAAKVSKMLKDY
DWINAERHLFGQPNSAYDFKTNNPKEAGQRLQKLQEMKEKLGRNVNMRAM
NVLTEAEERYNDLMKKKRIVENDKSKILTTIEDLDQKKNQALNIAWQKVN
KDFGSIFSTLLPGANAMLAPPEGQTVLDGLEFKVALGNTWKENLTELSGG
QRSLVALSLILSMLLFKPAPIYILDEVDAALDLSHTQNIGQMLRTHFTHS
QFIVVSLKEGMFNNANVLFKTKFVDGVSTVARFTQCQNGKISKEAKSKAK
PPKGAHVEV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50013
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee