cDNA Clone HARBI1/NAIF1

• Synonyms: C11orf77
• Plasmid: pcDNA5D FRT/TO HARBI1 3C FLAG 2A 2A 6His TEV NAIF1
• Parent Plasmid: pcDNA5D FRT/TO HARBI1 3C FLAG 2A 2A 6His TEV mcs
• Expressed: HARBI1 3C FLAG 2A 2A 6His TEV NAIF1
• Genbank: NM_173811.3 GI:224549031 (HARBI1) NM_197956.3 GI:215422409 (NAIF1)
• Tag: FLAG
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGCTATACCAATAACAGTGCTTGACTGTGACCTCTTGCTATATGGCCG
TGGTCACCGGACATTGGACCGTTTTAAGCTGGATGATGTGACTGATGAAT
ACTTGATGTCCATGTATGGGTTTCCACGGCAGTTCATTTATTACTTGGTG
GAGCTCTTGGGGGCGAATCTTTCTAGGCCTACTCAGCGATCCAGGGCTAT
TAGCCCAGAGACACAGGTCCTTGCAGCATTGGGTTTTTATACCTCAGGTT
CCTTCCAGACTCGGATGGGAGATGCCATTGGAATCAGTCAGGCGTCTATG
AGTCGTTGTGTTGCCAATGTCACTGAAGCACTTGTGGAAAGGGCCTCACA
GTTCATTCGCTTTCCAGCTGATGAAGCCTCCATTCAGGCTCTGAAGGATG
AATTCTATGGGTTGGCAGGGATGCCAGGGGTGATGGGGGTGGTTGACTGT
ATCCATGTGGCCATCAAGGCACCAAATGCTGAAGACCTCTCCTATGTGAA
CCGAAAAGGCCTGCATTCTTTAAACTGCCTGATGGTGTGTGACATTAGAG
GGACACTAATGACCGTGGAGACAAACTGGCCCGGCAGCCTACAGGACTGT
GCTGTGCTGCAGCAGTCTTCCCTCAGTAGTCAGTTTGAAGCGGGTATGCA
CAAAGATAGCTGGCTTCTGGGTGACAGTTCCTTCTTTCTTCGAACCTGGC
TCATGACCCCACTTCACATTCCTGAAACTCCAGCAGAATATCGCTATAAC
ATGGCCCATTCTGCAACTCACAGTGTGATTGAGAAGACTTTCCGAACCCT
CTGCTCCCGATTCCGCTGCCTGGATGGATCCAAGGGGGCACTGCAGTACT
CACCAGAGAAATCCAGCCATATCATCTTGGCCTGTTGTGTCCTCCACAAC
ATCTCCCTGGAGCATGGGATGGATGTTTGGTCCTCTCCAATGACAGGACC
CATGGAACAGCCCCCGGAAGAGGAGTATGAGCACATGGAGTCCCTGGACT
TAGAGGCTGACCGTATTCGTCAGGAGCTAATGCTCACTCATTTTAGCGCG
GCCGCGCTGGAAGTTCTGTTCCAAGGACCCGACTACAAGGACGACGATGA
CAAGGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATC
CCGGCCCAGAAGGGAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAG
AATCCCGGCCCACACCATCACCATCACCATGAAAACCTGTATTTTCAGAC
CGGTATGGCCGTCCCAGCCAAGAAAAGGAAGATGAACTTCTCAGAGCGGG
AGGTGGAGATCATCGTGGAGGAGCTGGAGCTGAAGAAGCACCTGCTGGTG
AACCACTTCAACGCCGGGGTACCCCTGGCCGCCAAGAGTGCGGCCTGGCA
CGGCATCCTGAGAAGGGTCAACGCCGTGGCCACCTGCCGCAGAGAGCTGC
CTGAGGTCAAGAAGAAGTGGTCTGACCTCAAGACCGAGGTCCGTCGCAAG
GTTGCCCAGGTCCGGGCCGCCGTGGAGGGTGGTGAGGCGCCGGGGCCCAC
TGAGGAGGACGGAGCTGGGGGGCCTGGGACAGGCGGTGGCAGTGGCGGCG
GTGGCCCAGCTGTAGCCCCAGTGCTGCTGACCCCCATGCAACAACGTATC
TGCAACCTGCTGGGCGAGGCCACCATCATCAGCCTGCCCAGCACCACAGA
GATCCACCCTGTGGCCCTCGGACCCTCGGCCACCGCAGCCGCAGCCACGG
TCACCCTGACACAGATCCCCACAGAGACCACCTATCACACGCTGGAGGAG
GGCGTGGTGGAGTACTGCACGGCTGAGGCGCCCCCACCTCTGCCACCAGA
GACCCCTGTGGACATGATGGCCCAGCATGCAGACACGTCGGTCAAGCCGC
AAGCGCTCAAGAGCCGCATTGCTCTCAACTCCGCCAAGCTGATACAGGAG
CAGCGGGTCACCAACCTGCATGTGAAGGAGATCGCACAGCACCTGGAACA
GCAGAACGACCTACTGCAGATGATCCGCCGCTCCCAGGAAGTGCAGGCCT
GTGCCCAGGAGCGCCAGGCCCAGGCCATGGAGGGCACACAGGCTGCCCTG
AGCGTCCTCATCCAGGTCCTCCGGCCTATGATCAAAGATTTCCGCCGCTA
CCTGCAGAGCAACACAGCTAACCCGGCCCCCGCCTCTGACCCTGGGCAGG
TGGCCCAGAATGGGCAGCCAGACAGCATCATCCAGTGA

Amino Acid Sequence

MAIPITVLDCDLLLYGRGHRTLDRFKLDDVTDEYLMSMYGFPRQFIYYLV
ELLGANLSRPTQRSRAISPETQVLAALGFYTSGSFQTRMGDAIGISQASM
SRCVANVTEALVERASQFIRFPADEASIQALKDEFYGLAGMPGVMGVVDC
IHVAIKAPNAEDLSYVNRKGLHSLNCLMVCDIRGTLMTVETNWPGSLQDC
AVLQQSSLSSQFEAGMHKDSWLLGDSSFFLRTWLMTPLHIPETPAEYRYN
MAHSATHSVIEKTFRTLCSRFRCLDGSKGALQYSPEKSSHIILACCVLHN
ISLEHGMDVWSSPMTGPMEQPPEEEYEHMESLDLEADRIRQELMLTHFSA
AALEVLFQGPDYKDDDDKEGRGSLLTCGDVEENPGPEGRGSLLTCGDVEE
NPGPHHHHHHENLYFQTGMAVPAKKRKMNFSEREVEIIVEELELKKHLLV
NHFNAGVPLAAKSAAWHGILRRVNAVATCRRELPEVKKKWSDLKTEVRRK
VAQVRAAVEGGEAPGPTEEDGAGGPGTGGGSGGGGPAVAPVLLTPMQQRI
CNLLGEATIISLPSTTEIHPVALGPSATAAAATVTLTQIPTETTYHTLEE
GVVEYCTAEAPPPLPPETPVDMMAQHADTSVKPQALKSRIALNSAKLIQE
QRVTNLHVKEIAQHLEQQNDLLQMIRRSQEVQACAQERQAQAMEGTQAAL
SVLIQVLRPMIKDFRRYLQSNTANPAPASDPGQVAQNGQPDSIIQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50074
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bicistronic plasmid that should autocleave at 2A 2A sequence.