cDNA Clone Mylk mouse

• Synonyms: AW489456, KRP, MLCK108, MLCK210, Mlck, nmMlck, smMLCK
• Plasmid: pSCB MYLK mouse Sal Not 4xerror
• Parent Plasmid: pSC-B
• Genbank: NM_139300.3
• Species: Mouse

Sequence of Insert

GTCGACGCCATGTCTGGCCAGTTACCTGGGAAGACCATGGGGGACGTGAAACTGTTTGCCTCGTCACACATGTCTAAAACCTCCCACAGTGTGGATCCCTCGAAGGTCAGCTCCATGCCTCTCACCGAGGCCCCAGCTTTCATTCTGCCCCCTCGGAACCTCTGCGTCAAAGAAGGAGCCACAGCCAAGTTCGAAGGGAGGGTCCGGGGCTACCCAGAGCCCCAGGTGACATGGCACAGAAAAGGGCAAGCCATCACCAATGGTGGCCGCTTCCTGCTGGACTGTGGAGTGCGGGGGACTTTTAGCCTTGTCATCCACACTGTCCGCGAGGAGGACAAGGGCAAATACACCTGTGAAGCTAGCAATGGCAGTGGCGCCCGCCAGGTGACCGTGGAGCTGACTGTGGAAGGGAATTCTATGAAGAAGCGTGATCAGCCTGTTCTTTCTAAAGCCTCAGGATTTCCAGGGGAGACTCGTCCTAGCATCTGGGGTGAGTGCCCACCAAAGTTTGCCACCAAGCTGGGCAGAGCTGTGGTCAAAGAAGGGCAGATGTGGCGATTCTCCTGCAAGATCACCGGCCGGCCTCCACCACAGGTCACCTGGCTCAAGGGAAATGTCCCGTTGCAGCCAAGTGCCCGCGTGTCAATGTCAGAGAAGAATGGCATGCAGATCCTGGAGATCCGTGGGGTCACTCGGGATGACCTGGGCGTGTACACCTGCATGGTGGTGAATGGGTCAGGGAAAGCCTCCATGTCGGCGGAGCTCTCCATCCCAGGTTTGGACAATGCCTCTAGGTTGGCTGTGAGAGGAACCAAAGCCCCCAGTCCAGACATCAGGAAGGAGGTGACCAATGGAGTCTCCAAAGACCCAGAGACTGTGGCTGAAAGTAAGAACTGTCCAAGTCCCCAGAGGAGTGGCTCCTCAGCCCGGGCCACCAACAGTCACCTCAAGTCCCCGCAGGAGCCCAAGCCAAAGCTGTGTGAGGATGCTCCCAGAAAGGTCCCGCAGTCCTCCATCCTGCAGAAGTCCACCAGCACCATCACTCTGCAGGCCTTGAAAGTCCAGCCAGAAGCGAGAGTACCAGCCATAGGGTCCTTCTCTCCTGGGGAAGACAGGAAGAGTCTGGCTGCCCCTCAGCAAGCCACACTCCCTACCAGACAGTCTAGCCTGGGAGGTTCCGTTGGGAACAAGTTTGTTACCGGGAACATCCCCAGAGAGAGCCAAAGGGAGTCAACATTCCCCAGATTTGAGAGTCAGCCCCAAAGCCAGGAGGTCACTGAAGGTCAGACAGTCAAGTTCATCTGCGAGGTTTCAGGTATACCAAAGCCTGACGTGGGCTGGTTCCTGGAAGGCATCCCCGTGAGGAGACGGGAAGGCATCACTGAGGTTTATGAAGATGGTGTATCCCATCACCTGTGCCTGCTGAGAGCCCGGACGAGAGACAGTAGGAAGTACAGCTGCACAGCATCCAATAGCCTTGGCCAGGTGTCCTGCAGCTGGAGCCTGCTGGTGGACCGGCCGAACTTGGCACAGACAGCCCCTTCATTCTCTAGTGTTCTGAAGGACAGTGTTGTCATTGAGGGCCAGGATTTTGTGCTACGGTGCTCAGTGCAGGGGACTCCAGCACCCCGGGTCACGTGGCTGCTCAATGGACAGCCCATCCAGTTTGCTCACTCCATCTGTGAGGCAGGCGTGGCCGAGCTCCACATCCAGGATGCCCTGCCAGAGGACCGTGGCACCTACACCTGCCTGGCTGAGAATGCCATGGGACAGGTGTCCTGCAGCGCCACTGTCACCGTCCAAGAGAAGAAAGGTGAAGGAGAGAGGGAACACCGTCTGTCCCCAGCTCGCAGCAAGCCCATCGCACCCATCTTCCTGCAGGGCCTCTCCGACCTCAAAGTCATGGATGGAAGCCAGGTCACTATGACAGTCCAGGTGTCAGGGAACCCACCTCCGGAAGTCATCTGGCTTCACGACGGGAATGAGATTCAGGAGTCCGAGGACTTCCACTTCGAGCAGAAGGGTGGCTGGCACAGCCTGTGTATCCAGGAGGTGTTCCCGGAGGACACGGGCACGTACACCTGTGAGGCCTGGAACAGTGCCGGGGAGGTCCGCACCCGGGCCGTCCTCACCGTGCAAGAGCCTCATGATGGCACCCAGCCCTGGTTCATCAGCAAGCCTCGTTCGGTGACAGCCACCCTGGGCCAGAGTGTCCTCATCTCCTGCGCCATAGCTGGAGACCCCTTTCCCACCGTGCACTGGCTCCGGGATGGCAGAGCCCTCTCCAAGGACAGTGGCCACTTTGAGCTGCTCCAGAATGAGGACGTGTTCACCCTGGTTCTAAAGAACGTGCAGCCCTGGCATGCCGGCCAGTATGAGATTCTGCTCAAGAACCGGGTTGGCGAGTGTAGTTGCCAAGTGTCACTGATGCTTCACAACAGCCCTTCCAGAGCCCCACCACGGGGGCGGGAGCCTGCCAGCTGCGAGGGCCTCTGTGGTGGTGGAGGAGTTGGTGCTCATGGTGATGGTGACCGTCATGGGACCCTGAGGCCCTGCTGGCCAGCAAGAGGGCAAGGTTGGCCTGAGGAGGAAGACGGAGAAGACGTGAGGGGGCTGTTGAAGAGACGGGTGGAGACCAGGCTGCACACAGAAGAGGCCATCCGCCAGCAGGAGGTGGGGCAGCTGGATTTTCGAGACCTCCTGGGAAAGAAGGTGAGCACCAAGACCGTCTCAGAAGACGACCTGAAGGACATCCCAGCCGAGCAGATGGATTTCCGCGCCAACCTTCAGCGGCAAGTGAAGCCAAAGACCATATCCGAGGAAGAGAGGAAGGTGCATAGCCCTCAGCAGGTTGACTTCCGGTCTGTCCTCGCCAAGAAGGGGACCCCTAAGACCCCCGTTCCTGAGAAGGCACCTCCAAAAGCTGCCACCCCAGACTTTCGCTCTGTGCTAGGTGGCAAGAAGAAGTCACCCTCGGAGAATGGGGGCAACAGCGCTGAGGTCTTGAATGTCAAGGCTGGGGAAAGCCCCACACCTGCAGGCGATGCGCAGGCCATCGGGGCCCTGAAACCCGTAGGCAATGCCAAGCCTGCTGAGACCCCGAAACCTATAGGTAATGCCAAGCCTACCGAGACCCTGAAGCCTGTGGGCAACACCAAGCCTGCCGAGACCCTGAAACCCATAGCCAACGCACAGCCTTCAGGGTCCCTGAAACCTGTAACCAACGCACAACCTGCTGAGCCCCAGAAGCCTGTGGGCAATGCCAAGTCTGCTGAGACCTCGAAACCAGCTGGGAAAGAAGAAGTCAAGGAGGTAAAGAATGATGTGAACTGTAAGAAAGGGCAGGTCGGAGCCACGGGCAATGAAAAGCGACCCGAGAGCCAAGGCTCGGCCCCAGTTTTCAAGGAAAAGCTACAAGATGTCCATGTGGCAGAGGGGGAGAAGCTGCTACTCCAATGCCAGGTGATCTCTGATCCCCCCGCCACTGTCACCTGGTCGCTGAATGGGAAGACACTCAAGACCACAAAATTCATCGTCCTCGCCCAAGAAGGCTCACGTTTCTCTGTCTCCATCGAGAAGGCACTGCCTGAGGACCGAGGTTTGTACAAGTGTGTAGCCAAGAACAGCGCTGGCCAGGCAGAGTGTTCCTGCCAAGTCACTGTGGACGATGCTCAGACCAGTGAGAACACCAAGGCCCCGGAGATGAAATCCCGGAGACCCAAGAGCTCTCTTCCCCCTGTGCTAGGAACAGAGAGCGACGCAACTGTGAAAAAGAAACCTGCCCCCAAGACACCCACAAAAGCAGCTATGCCCCCTCAAATCATCCAGTTCCCCGAGGACCAGAAGGTGCGCGCAGGAGAACCTGTGGAGCTGTTTGGGAAAGTGGCTGGCACCCAGCCCATCACCTGCAAGTGGATGAAGTTCCGAAAGCAGATCCAGGAGAGCGAGCATATCAAGGTGGAGAATGGCGAGAGCGGCAGCAAGCTCACCATCCTGGCCGCGCGGCAGGAGCACTGTGGCTGCTACACACTGGTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAAGTCAACCTCACGGTCGTGGATAAGCCAGACCCCCCGGCTGGCACACCTTGTGCTTCTGACATTCGGAGTTCCTCACTGACCCTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAATGTGGAGATCTGGGACACAGAGGACAAGGTGTGGAAAGAGCTAGCCACGTGCCGCAGCACCTCTTTTAATGTCCAAGACCTGCTGCCTGACCGCGAGTACAAATTCCGAGTGCGTGCAGTCAACGTCTATGGAACCAGCGAGCCAAGCCAAGAGTCCGAGCTCACAGCTGTGGGAGAGAAACCTGAAGAGCCAAAGGATGAAGTGGAAGTGTCCGATGATGACGAAAAGGAACCTGAGGTTGATTACCGGACGGTGACTGTTAACACTGAACAGAAAGTGTCTGATGTGTATGACATTGAAGAGCGACTAGGATCTGGGAAGTTTGGACAAGTCTTCCGACTTGTGGAAAAGAAAACTGGAAAAATCTGGGCAGGGAAATTTTTCAAGGCCTATTCCGCCAAGGAGAAAGACAACATCCGTCAGGAGATCAGCATCATGAACTGCCTCCACCACCCCAAGCTGGTCCAGTGTGTGGATGCCTTCGAAGAAAAGGCCAACATTGTCATGGTTCTGGAGATCGTGTCCGGGGGTGAGCTGTTTGAGCGTATCATTGACGAGGACTTCGAGCTGACGGAGCGGGAGTGTATCAAGTACATGAGGCAGATCTCGGAAGGGGTGGAGTACATCCACAAGCAGGGCATTGTGCACCTGGACCTCAAGCCCGAGAACATCATGTGTGTCAACAAGACAGGCACCAGGATCAAGCTCATTGACTTCGGCCTGGCACGGAGACTCGAGAACGCTGGGTCTCTGAAGGTCCTCTTTGGGACCCCAGAGTTTGTGGCTCCTGAAGTGATCAACCATGAGCCCATCGGCTACGCCACAGACATGTGGAGCATCGGGGTCATCTGCTATATTCTGGTCAGCGGACTTTCTCCCTTCATGGGTGACAATGATAACGAGACCTTAGCCAACGTCACTTCAGCCACCTGGGACTTCGATGACGAGGCGTTCGATGAGATCTCCGATGACGCAAAGGATTTCATCAGTAACTTGCTGAAGAAAGATATGAAAAACCGTCTGGACTGCACCCAGTGCCTTCAGCACCCATGGCTGATGAAAGACACCAAGAACATGGAGGCCAAGAAGCTGTCCAAGGACCGGATGAAGAAATACATGGCAAGAAGGAAGTGGCAGAAAACGGGCAATGCTGTGAGAGCCATCGGGAGACTGTCCTCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCTTCAACAGGGTCACCAACCAGCCCAATCAATGCGGAGAAACTAGAGTCTGAAGACGATGTCTCCCAAGCTTTCCTGGAGGCTGTTGCTGAGGAAAAGCCCCATGTGAAACCTTATTTCTCTAAGACCATCCGTGATCTGGAGGTCGTGGAGGGAAGCGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCTGAGGTGGTCTGGTTCAAGGATGACCAATCAATCAGGGAGTCCCGCCACTTCCAGATAGACTACGACGAAGACGGTAACTGCTCGCTCATCATCAGCGACGTCTGTGGGGATGATGACGCCAAGTACACCTGCAAGGCTGTCAACAGCCTCGGAGAAGCCACCTGCACTGCGGAGCTCATCGTGGAGACCATGGAGGAAGGGGAAGGGGAGGAGGGGGGAGAGGAGGAGGAGGAGGAGGAGGAGTGAGCGGCCGC

Amino Acid Sequence

MSGQLPGKTMGDVKLFASSHMSKTSHSVDPSKVSSMPLTEAPAFILPPRN
LCVKEGATAKFEGRVRGYPEPQVTWHRKGQAITNGGRFLLDCGVRGTFSL
VIHTVREEDKGKYTCEASNGSGARQVTVELTVEGNSMKKRDQPVLSKASG
FPGETRPSIWGECPPKFATKLGRAVVKEGQMWRFSCKITGRPPPQVTWLK
GNVPLQPSARVSMSEKNGMQILEIRGVTRDDLGVYTCMVVNGSGKASMSA
ELSIPGLDNASRLAVRGTKAPSPDIRKEVTNGVSKDPETVAESKNCPSPQ
RSGSSARATNSHLKSPQEPKPKLCEDAPRKVPQSSILQKSTSTITLQALK
VQPEARVPAIGSFSPGEDRKSLAAPQQATLPTRQSSLGGSVGNKFVTGNI
PRESQRESTFPRFESQPQSQEVTEGQTVKFICEVSGIPKPDVGWFLEGIP
VRRREGITEVYEDGVSHHLCLLRARTRDSRKYSCTASNSLGQVSCSWSLL
VDRPNLAQTAPSFSSVLKDSVVIEGQDFVLRCSVQGTPAPRVTWLLNGQP
IQFAHSICEAGVAELHIQDALPEDRGTYTCLAENAMGQVSCSATVTVQEK
KGEGEREHRLSPARSKPIAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPE
VIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGE
VRTRAVLTVQEPHDGTQPWFISKPRSVTATLGQSVLISCAIAGDPFPTVH
WLRDGRALSKDSGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGEC
SCQVSLMLHNSPSRAPPRGREPASCEGLCGGGGVGAHGDGDRHGTLRPCW
PARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGK
KVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTISEEERKVHSPQQVDF
RSVLAKKGTPKTPVPEKAPPKAATPDFRSVLGGKKKSPSENGGNSAEVLN
VKAGESPTPAGDAQAIGALKPVGNAKPAETPKPIGNAKPTETLKPVGNTK
PAETLKPIANAQPSGSLKPVTNAQPAEPQKPVGNAKSAETSKPAGKEEVK
EVKNDVNCKKGQVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQC
QVISDPPATVTWSLNGKTLKTTKFIVLAQEGSRFSVSIEKALPEDRGLYK
CVAKNSAGQAECSCQVTVDDAQTSENTKAPEMKSRRPKSSLPPVLGTESD
ATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVAGTQPITC
KWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSR
QAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVE
IWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQES
ELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEER
LGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQEISIMNCLHHP
KLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQI
SEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLK
VLFGTPEFVAPEVINHEPIGYATDMWSIGVICYILVSGLSPFMGDNDNET
LANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWL
MKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSG
RKSSTGSPTSPINAEKLESEDDVSQAFLEAVAEEKPHVKPYFSKTIRDLE
VVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLII
SDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEEE
*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48769
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains 4 amino acid changes which require repair; G182W, G480R, R481K and Y1666H. Also contains one known benign variation G261S (ensembl) along with 9 silent nucleotide changes T>C 297, T>C 567, T>C 630, A>G 1263, T>C 1689, G>A 1761, A>G 5145, A>G 5550 and C>T 5682 bp.