cDNA Clone USP33

• Synonyms: VDU1
• Plasmid: pSC-B USP33 (no stop) C194A
• Parent Plasmid: pSC-B
• Genbank: NM_201626.2 GI:391738247 with insertion of residues RFVVSCVP after K551 (present in many other published sequences for USP33)
• Species: Human

Sequence of Insert

GGATCCATGACAGGATCAAATTCACACATAACGATATTAACCTTAAAGGT
GTTACCTCATTTTGAAAGTCTTGGGAAACAGGAAAAAATTCCTAACAAAA
TGTCAGCTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGAAATA
ACAAAAGAAGATTTGATACAAAAATCCCTTGGTACTTGTCAGGATTGTAA
AGTCCAAGGACCAAATCTTTGGGCATGTCTGGAGAATAGATGTTCATATG
TTGGCTGTGGTGAATCACAAGTAGATCACAGCACCATACATTCTCAGGAG
ACAAAGCATTATCTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTA
TGCTTGCAGCAAAGAAGTATTTTTGGATAGGAAATTAGGAACTCAGCCTT
CATTGCCTCATGTAAGACAACCTCACCAAATACAAGAAAACAGTGTCCAG
GATTTTAAAATACCCAGTAATACAACATTAAAAACTCCTCTGGTTGCCGT
ATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATGAACTTAGGGCCA
GAGGTCTTACAGGTTTGAAAAATATAGGAAATACTgcTTACATGAATGCA
GCTTTGCAGGCTCTTTCTAATTGCCCACCTTTGACACAGTTTTTTCTTGA
TTGTGGAGGACTAGCTCGAACAGATAAGAAACCTGCCATTTGTAAAAGTT
ATCTCAAACTAATGACAGAGCTGTGGCATAAAAGCAGGCCAGGATCTGTT
GTGCCTACTACTCTGTTTCAAGGAATTAAAACTGTAAATCCAACATTTCG
GGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGATGTTTAATGGATT
TGCTTCATGAAGAATTGAAAGAGCAAGTCATGGAAGTAGAAGAAGATCCG
CAAACCATAACCACTGAGGAGACAATGGAAGAAGACAAGAGCCAGTCGGA
TGTAGATTTTCAGTCTTGTGAATCTTGTAGCAACAGTGATAGAGCAGAAA
ATGAAAATGGCTCTAGATGCTTTTCTGAAGATAATAATGAAACAACAATG
TTAATTCAGGATGATGAAAACAATTCAGAAATGTCAAAGGATTGGCAAAA
AGAGAAGATGTGCAATAAGATTAATAAAGTAAATTCTGAAGGCGAATTTG
ATAAAGATAGAGACTCTATATCTGAAACAGTCGACTTAAACAACCAGGAA
ACTGTCAAAGTGCAAATACACAGCAGAGCTTCAGAATATATCACTGATGT
CCATTCGAATGACCTGTCTACACCACAGATCCTTCCATCAAATGAAGGTG
TTAATCCACGTTTATCGGCAAGCCCTCCTAAATCAGGCAATTTGTGGCCA
GGATTGGCACCACCACACAAAAAAGCTCAGTCTGCATCTCCAAAGAGAAA
AAAACAGCACAAGAAATACAGAAGTGTTATTTCAGACATATTTGATGGAA
CAATCATTAGTTCAGTGCAGTGTCTGACTTGTGACAGGGTGTCTGTAACC
CTCGAGACCTTTCAAGATCTGTCCTTGCCAATTCCTGGCAAGGAAGACCT
TGCTAAGCTGCATTCATCAAGTCATCCAACTTCTATAGTCAAAGCAGGAT
CATGTGGCGAAGCATATGCTCCACAAGGGTGGATAGCTTTTTTCATGGAA
TATGTGAAGAGGTTTGTTGTCTCATGTGTCCCTAGCTGGTTTTGGGGTCC
AGTAGTAACCTTGCAAGATTGTCTTGCTGCCTTCTTTGCCAGAGATGAAC
TAAAAGGTGACAATATGTACAGTTGTGAAAAATGCAAAAAGTTGAGAAAT
GGAGTGAAGTTTTGTAAAGTACAAAACTTTCCTGAGATTTTGTGCATCCA
CCTTAAAAGATTCAGACATGAACTAATGTTTTCCACCAAAATCAGTACCC
ATGTTTCATTTCCGCTAGAAGGCTTGGATCTTCAGCCATTTCTTGCTAAG
GATAGTCCAGCTCAAATTGTGACATATGATCTTCTGTCAGTCATTTGCCA
TCATGGAACTGCAAGTAGTGGACACTATATAGCCTACTGCCGAAACAATC
TAAATAATCTCTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCA
GAATCTACTGTACAAAATGCAGAAGCTTACGTTCTTTTCTATAGGAAGAG
CAGCGAAGAGGCACAAAAAGAGAGGAGAAGGATATCAAATTTATTGAACA
TAATGGAACCAAGCCTCCTTCAGTTTTATATTTCTCGACAGTGGCTTAAT
AAATTTAAGACCTTTGCCGAACCTGGCCCTATTTCAAATAATGACTTTCT
TTGTATTCATGGAGGTGTTCCTCCAAGAAAAGCTGGTTATATTGAAGACC
TGGTTTTGATGCTGCCTCAGAACATTTGGGATAACCTATATAGCAGGTAT
GGTGGAGGACCAGCTGTCAACCATCTGTACATTTGTCATACTTGCCAAAT
TGAGGCGGAGAAAATTGAAAAAAGAAGAAAAACTGAATTGGAAATTTTTA
TTCGGGTAAAAAAGGCGGCCGC

Amino Acid Sequence

MTGSNSHITILTLKVLPHFESLGKQEKIPNKMSAFRNHCPHLDSVGEITK
EDLIQKSLGTCQDCKVQGPNLWACLENRCSYVGCGESQVDHSTIHSQETK
HYLTVNLTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPHQIQENSVQDF
KIPSNTTLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIGNTAYMNAAL
QALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVP
TTLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEVEEDPQT
ITTEETMEEDKSQSDVDFQSCESCSNSDRAENENGSRCFSEDNNETTMLI
QDDENNSEMSKDWQKEKMCNKINKVNSEGEFDKDRDSISETVDLNNQETV
KVQIHSRASEYITDVHSNDLSTPQILPSNEGVNPRLSASPPKSGNLWPGL
APPHKKAQSASPKRKKQHKKYRSVISDIFDGTIISSVQCLTCDRVSVTLE
TFQDLSLPIPGKEDLAKLHSSSHPTSIVKAGSCGEAYAPQGWIAFFMEYV
KRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGV
KFCKVQNFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDS
PAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSES
TVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKF
KTFAEPGPISNNDFLCIHGGVPPRKAGYIEDLVLMLPQNIWDNLYSRYGG
GPAVNHLYICHTCQIEAEKIEKRRKTELEIFIRVKK

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU24715
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation at Ile190 compared to NCBI ref