cDNA Clone USP33

• Synonyms: VDU1
• Plasmid: pcDNA5D FRT/TO BirA R118G-USP33 C194A-HA (codon optimised seq)
• Parent Plasmid: pcDNA5D FRT/TO BirA R118G
• Expressed: BirA R118G-USP33 C194A-HA
• Genbank: NM_201626.2 GI:391738247 with insertion of residues RFVVSCVP after K551 (present in many other published sequences for USP33)
• Tag: HA
• Tag: BirA
• Species: Human

Sequence of Insert

GGATCCATGACAGGATCAAATTCACACATAACGATATTAACCTTAAAGGT
GTTACCTCATTTTGAAAGTCTTGGGAAACAGGAAAAAATTCCTAACAAAA
TGTCAGCTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGAAATA
ACAAAAGAAGATTTGATACAAAAATCCCTTGGTACTTGTCAGGATTGTAA
AGTCCAAGGACCAAATCTTTGGGCATGTCTGGAGAATAGATGTTCATATG
TTGGCTGTGGTGAATCACAAGTAGATCACAGCACCATACATTCTCAGGAG
ACAAAGCATTATCTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTA
TGCTTGCAGCAAAGAAGTATTTTTGGATAGGAAATTAGGAACTCAGCCTT
CATTGCCTCATGTAAGACAACCTCACCAAATACAAGAAAACAGTGTCCAG
GATTTTAAAATACCCAGTAATACAACATTAAAAACTCCTCTGGTTGCCGT
ATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATGAACTTAGGGCCA
GAGGTCTTACAGGTTTGAAAAATATAGGAAATACTgcTTACATGAATGCA
GCTTTGCAGGCTCTTTCTAATTGCCCACCTTTGACACAGTTTTTTCTTGA
TTGTGGAGGACTAGCTCGAACAGATAAGAAACCTGCCATTTGTAAAAGTT
ATCTCAAACTAATGACAGAGCTGTGGCATAAAAGCAGGCCAGGATCTGTT
GTGCCTACTACTCTGTTTCAAGGAATTAAAACTGTAAATCCAACATTTCG
GGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGATGTTTAATGGATT
TGCTTCATGAAGAATTGAAAGAGCAAGTCATGGAAGTAGAAGAAGATCCG
CAAACCATAACCACTGAGGAGACAATGGAAGAAGACAAGAGCCAGTCGGA
TGTAGATTTTCAGTCTTGTGAATCTTGTAGCAACAGTGATAGAGCAGAAA
ATGAAAATGGCTCTAGATGCTTTTCTGAAGATAATAATGAAACAACAATG
TTAATTCAGGATGATGAAAACAATTCAGAAATGTCAAAGGATTGGCAAAA
AGAGAAGATGTGCAATAAGATTAATAAAGTAAATTCTGAAGGCGAATTTG
ATAAAGATAGAGACTCTATATCTGAAACAGTCGACTTAAACAACCAGGAA
ACTGTCAAAGTGCAAATACACAGCAGAGCTTCAGAATATATCACTGATGT
CCATTCGAATGACCTGTCTACACCACAGATCCTTCCATCAAATGAAGGTG
TTAATCCACGTTTATCGGCAAGCCCTCCTAAATCAGGCAATTTGTGGCCA
GGATTGGCACCACCACACAAAAAAGCTCAGTCTGCATCTCCAAAGAGAAA
AAAACAGCACAAGAAATACAGAAGTGTTATTTCAGACATATTTGATGGAA
CAATCATTAGTTCAGTGCAGTGTCTGACTTGTGACAGGGTGTCTGTAACC
CTCGAGACCTTTCAAGATCTGTCCTTGCCAATTCCTGGCAAGGAAGACCT
TGCTAAGCTGCATTCATCAAGTCATCCAACTTCTATAGTCAAAGCAGGAT
CATGTGGCGAAGCATATGCTCCACAAGGGTGGATAGCTTTTTTCATGGAA
TATGTGAAGAGGTTTGTTGTCTCATGTGTCCCTAGCTGGTTTTGGGGTCC
AGTAGTAACCTTGCAAGATTGTCTTGCTGCCTTCTTTGCCAGAGATGAAC
TAAAAGGTGACAATATGTACAGTTGTGAAAAATGCAAAAAGTTGAGAAAT
GGAGTGAAGTTTTGTAAAGTACAAAACTTTCCTGAGATTTTGTGCATCCA
CCTTAAAAGATTCAGACATGAACTAATGTTTTCCACCAAAATCAGTACCC
ATGTTTCATTTCCGCTAGAAGGCTTGGATCTTCAGCCATTTCTTGCTAAG
GATAGTCCAGCTCAAATTGTGACATATGATCTTCTGTCAGTCATTTGCCA
TCATGGAACTGCAAGTAGTGGACACTATATAGCCTACTGCCGAAACAATC
TAAATAATCTCTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCA
GAATCTACTGTACAAAATGCAGAAGCTTACGTTCTTTTCTATAGGAAGAG
CAGCGAAGAGGCACAAAAAGAGAGGAGAAGGATATCAAATTTATTGAACA
TAATGGAACCAAGCCTCCTTCAGTTTTATATTTCTCGACAGTGGCTTAAT
AAATTTAAGACCTTTGCCGAACCTGGCCCTATTTCAAATAATGACTTTCT
TTGTATTCATGGAGGTGTTCCTCCAAGAAAAGCTGGTTATATTGAAGACC
TGGTTTTGATGCTGCCTCAGAACATTTGGGATAACCTATATAGCAGGTAT
GGTGGAGGACCAGCTGTCAACCATCTGTACATTTGTCATACTTGCCAAAT
TGAGGCGGAGAAAATTGAAAAAAGAAGAAAAACTGAATTGGAAATTTTTA
TTCGGGTAAAAAAGGCGGCCGC

Amino Acid Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMTGSNSHITILTLKVLPHFESLGKQEK
IPNKMSAFRNHCPHLDSVGEITKEDLIQKSLGTCQDCKVQGPNLWACLEN
RCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKL
GTQPSLPHVRQPHQIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEED
ELRARGLTGLKNIGNTAYMNAALQALSNCPPLTQFFLDCGGLARTDKKPA
ICKSYLKLMTELWHKSRPGSVVPTTLFQGIKTVNPTFRGYSQQDAQEFLR
CLMDLLHEELKEQVMEVEEDPQTITTEETMEEDKSQSDVDFQSCESCSNS
DRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQKEKMCNKINKVNS
EGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDLSTPQILP
SNEGVNPRLSASPPKSGNLWPGLAPPHKKAQSASPKRKKQHKKYRSVISD
IFDGTIISSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTSI
VKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFF
ARDELKGDNMYSCEKCKKLRNGVKFCKVQNFPEILCIHLKRFRHELMFST
KISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAY
CRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRIS
NLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGVPPRKAG
YIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTE
LEIFIRVKKAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24736
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 49108 parental. Silent mutation at Ile190 compared to NCBI ref