cDNA Clone MYLK (mouse)

• Synonyms: AW489456, KRP, MLCK108, MLCK210, Mlck, nmMlck, smMLCK
• Plasmid: pSCB MYLKm G1294V Sal Not
• Parent Plasmid: pSC-B
• Genbank: NM_139300.3
• Species: Mouse

Sequence of Insert

GTCGACGCCATGTCTGGCCAGTTACCTGGGAAGACCATGGGGGACGTGAAACTGTTTGCCTCGTCACACATGTCTAAAACCTCCCACAGTGTGGATCCCTCGAAGGTCAGCTCCATGCCTCTCACCGAGGCCCCAGCTTTCATTCTGCCCCCTCGGAACCTCTGCGTCAAAGAAGGAGCCACAGCCAAGTTCGAAGGGAGGGTCAGGGGCTACCCAGAGCCCCAGGTGACATGGCACAGAAAAGGGCAAGCCATCACCAATGGTGGCCGCTTCCTGCTGGACTGTGGAGTGCGGGGGACTTTTAGCCTTGTCATCCACACTGTCCGCGAGGAGGACAAGGGCAAATACACCTGTGAAGCTAGCAATGGCAGTGGCGCCCGCCAGGTGACCGTGGAGCTGACTGTGGAAGGGAATTCTATGAAGAAGCGTGATCAGCCTGTTCTTTCTAAAGCCTCAGGATTTCCAGGGGAGACTCGTCCTAGCATCTGGGGTGAGTGCCCACCAAAGTTTGCCACCAAGCTGGGCAGAGCTGTGGTCAAAGAAGGGCAGATGGGGCGATTCTCCTGCAAGATCACCGGCCGGCCTCCACCACAGGTCACCTGGCTCAAGGGAAATGTCCCGTTGCAGCCAAGTGCCCGCGTGTCAATGTCAGAGAAGAATGGCATGCAGATCCTGGAGATCCGTGGGGTCACTCGGGATGACCTGGGCGTGTACACCTGCATGGTGGTGAATGGGTCAGGGAAAGCCTCCATGTCGGCGGAGCTCTCCATCCCAGGTTTGGACAATGCCTCTAGGTTGGCTGTGAGAGGAACCAAAGCCCCCAGTCCAGACATCAGGAAGGAGGTGACCAATGGAGTCTCCAAAGACCCAGAGACTGTGGCTGAAAGTAAGAACTGTCCAAGTCCCCAGAGGAGTGGCTCCTCAGCCCGGGCCACCAACAGTCACCTCAAGTCCCCGCAGGAGCCCAAGCCAAAGCTGTGTGAGGATGCTCCCAGAAAGGTCCCGCAGTCCTCCATCCTGCAGAAGTCCACCAGCACCATCACTCTGCAGGCCTTGAAAGTCCAGCCAGAAGCGAGAGTACCAGCCATAGGGTCCTTCTCTCCTGGGGAAGACAGGAAGAGTCTGGCTGCCCCTCAGCAAGCCACACTCCCTACCAGACAGTCTAGCCTGGGAGGTTCCGTTGGGAACAAGTTTGTTACCGGGAACATCCCCAGAGAGAGCCAAAGGGAGTCAACATTCCCCAGATTTGAGAGTCAGCCCCAAAGCCAGGAGGTCACTGAAGGTCAGACAGTCAAGTTCATCTGCGAGGTTTCAGGTATACCAAAGCCTGACGTGGGCTGGTTCCTGGAAGGCATCCCCGTGAGGAGACGGGAAGGCATCACTGAGGTTTATGAAGATGGTGTATCCCATCACCTGTGCCTGCTGAGAGCCCGGACGAGAGACAGTGGGAGGTACAGCTGCACAGCATCCAATAGCCTTGGCCAGGTGTCCTGCAGCTGGAGCCTGCTGGTGGACCGGCCGAACTTGGCACAGACAGCCCCTTCATTCTCTAGTGTTCTGAAGGACAGTGTTGTCATTGAGGGCCAGGATTTTGTGCTACGGTGCTCAGTGCAGGGGACTCCAGCACCCCGGGTCACGTGGCTGCTCAATGGACAGCCCATCCAGTTTGCTCACTCCATCTGTGAGGCAGGCGTGGCCGAGCTCCACATCCAGGATGCCCTGCCAGAGGACCGTGGCACCTACACCTGCCTGGCTGAGAATGCCATGGGACAGGTGTCCTGCAGCGCCACTGTCACCGTCCAAGAGAAGAAAGGTGAAGGAGAGAGGGAACACCGTCTGTCCCCAGCTCGCAGCAAGCCCATCGCACCCATCTTCCTGCAGGGCCTCTCCGACCTCAAAGTCATGGATGGAAGCCAGGTCACTATGACAGTCCAGGTGTCAGGGAACCCACCTCCGGAAGTCATCTGGCTTCACGACGGGAATGAGATTCAGGAGTCCGAGGACTTCCACTTCGAGCAGAAGGGTGGCTGGCACAGCCTGTGTATCCAGGAGGTGTTCCCGGAGGACACGGGCACGTACACCTGTGAGGCCTGGAACAGTGCCGGGGAGGTCCGCACCCGGGCCGTCCTCACCGTGCAAGAGCCTCATGATGGCACCCAGCCCTGGTTCATCAGCAAGCCTCGTTCGGTGACAGCCACCCTGGGCCAGAGTGTCCTCATCTCCTGCGCCATAGCTGGAGACCCCTTTCCCACCGTGCACTGGCTCCGGGATGGCAGAGCCCTCTCCAAGGACAGTGGCCACTTTGAGCTGCTCCAGAATGAGGACGTGTTCACCCTGGTTCTAAAGAACGTGCAGCCCTGGCATGCCGGCCAGTATGAGATTCTGCTCAAGAACCGGGTTGGCGAGTGTAGTTGCCAAGTGTCACTGATGCTTCACAACAGCCCTTCCAGAGCCCCACCACGGGGGCGGGAGCCTGCCAGCTGCGAGGGCCTCTGTGGTGGTGGAGGAGTTGGTGCTCATGGTGATGGTGACCGTCATGGGACCCTGAGGCCCTGCTGGCCAGCAAGAGGGCAAGGTTGGCCTGAGGAGGAAGACGGAGAAGACGTGAGGGGGCTGTTGAAGAGACGGGTGGAGACCAGGCTGCACACAGAAGAGGCCATCCGCCAGCAGGAGGTGGGGCAGCTGGATTTTCGAGACCTCCTGGGAAAGAAGGTGAGCACCAAGACCGTCTCAGAAGACGACCTGAAGGACATCCCAGCCGAGCAGATGGATTTCCGCGCCAACCTTCAGCGGCAAGTGAAGCCAAAGACCATATCCGAGGAAGAGAGGAAGGTGCATAGCCCTCAGCAGGTTGACTTCCGGTCTGTCCTCGCCAAGAAGGGGACCCCTAAGACCCCCGTTCCTGAGAAGGCACCTCCAAAAGCTGCCACCCCAGACTTTCGCTCTGTGCTAGGTGGCAAGAAGAAGTCACCCTCGGAGAATGGGGGCAACAGCGCTGAGGTCTTGAATGTCAAGGCTGGGGAAAGCCCCACACCTGCAGGCGATGCGCAGGCCATCGGGGCCCTGAAACCCGTAGGCAATGCCAAGCCTGCTGAGACCCCGAAACCTATAGGTAATGCCAAGCCTACCGAGACCCTGAAGCCTGTGGGCAACACCAAGCCTGCCGAGACCCTGAAACCCATAGCCAACGCACAGCCTTCAGGGTCCCTGAAACCTGTAACCAACGCACAACCTGCTGAGCCCCAGAAGCCTGTGGGCAATGCCAAGTCTGCTGAGACCTCGAAACCAGCTGGGAAAGAAGAAGTCAAGGAGGTAAAGAATGATGTGAACTGTAAGAAAGGGCAGGTCGGAGCCACGGGCAATGAAAAGCGACCCGAGAGCCAAGGCTCGGCCCCAGTTTTCAAGGAAAAGCTACAAGATGTCCATGTGGCAGAGGGGGAGAAGCTGCTACTCCAATGCCAGGTGATCTCTGATCCCCCCGCCACTGTCACCTGGTCGCTGAATGGGAAGACACTCAAGACCACAAAATTCATCGTCCTCGCCCAAGAAGGCTCACGTTTCTCTGTCTCCATCGAGAAGGCACTGCCTGAGGACCGAGGTTTGTACAAGTGTGTAGCCAAGAACAGCGCTGGCCAGGCAGAGTGTTCCTGCCAAGTCACTGTGGACGATGCTCAGACCAGTGAGAACACCAAGGCCCCGGAGATGAAATCCCGGAGACCCAAGAGCTCTCTTCCCCCTGTGCTAGGAACAGAGAGCGACGCAACTGTGAAAAAGAAACCTGCCCCCAAGACACCCACAAAAGCAGCTATGCCCCCTCAAATCATCCAGTTCCCCGAGGACCAGAAGGTGCGCGCAGGAGAACCTGTGGAGCTGTTTGGGAAAGTGGCTGTCACCCAGCCCATCACCTGCAAGTGGATGAAGTTCCGAAAGCAGATCCAGGAGAGCGAGCATATCAAGGTGGAGAATGGCGAGAGCGGCAGCAAGCTCACCATCCTGGCCGCGCGGCAGGAGCACTGTGGCTGCTACACACTGGTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAAGTCAACCTCACGGTCGTGGATAAGCCAGACCCCCCGGCTGGCACACCTTGTGCTTCTGACATTCGGAGTTCCTCACTGACCCTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAATGTGGAGATCTGGGACACAGAGGACAAGGTGTGGAAAGAGCTAGCCACGTGCCGCAGCACCTCTTTTAATGTCCAAGACCTGCTGCCTGACCGCGAGTACAAATTCCGAGTGCGTGCAGTCAACGTCTATGGAACCAGCGAGCCAAGCCAAGAGTCCGAGCTCACAGCTGTGGGAGAGAAACCTGAAGAGCCAAAGGATGAAGTGGAAGTGTCCGATGATGACGAAAAGGAACCTGAGGTTGATTACCGGACGGTGACTGTTAACACTGAACAGAAAGTGTCTGATGTGTATGACATTGAAGAGCGACTAGGATCTGGGAAGTTTGGACAAGTCTTCCGACTTGTGGAAAAGAAAACTGGAAAAATCTGGGCAGGGAAATTTTTCAAGGCCTATTCCGCCAAGGAGAAAGACAACATCCGTCAGGAGATCAGCATCATGAACTGCCTCCACCACCCCAAGCTGGTCCAGTGTGTGGATGCCTTCGAAGAAAAGGCCAACATTGTCATGGTTCTGGAGATCGTGTCCGGGGGTGAGCTGTTTGAGCGTATCATTGACGAGGACTTCGAGCTGACGGAGCGGGAGTGTATCAAGTACATGAGGCAGATCTCGGAAGGGGTGGAGTACATCCACAAGCAGGGCATTGTGCACCTGGACCTCAAGCCCGAGAACATCATGTGTGTCAACAAGACAGGCACCAGGATCAAGCTCATTGACTTCGGCCTGGCACGGAGACTCGAGAACGCTGGGTCTCTGAAGGTCCTCTTTGGGACCCCAGAGTTTGTGGCTCCTGAAGTGATCAACTATGAGCCCATCGGCTACGCCACAGACATGTGGAGCATCGGGGTCATCTGCTATATTCTGGTCAGCGGACTTTCTCCCTTCATGGGTGACAATGATAACGAGACCTTAGCCAACGTCACTTCAGCCACCTGGGACTTCGATGACGAGGCGTTCGATGAGATCTCCGATGACGCAAAGGATTTCATCAGTAACTTGCTGAAGAAAGATATGAAAAACCGTCTGGACTGCACCCAGTGCCTTCAGCACCCATGGCTGATGAAAGACACCAAGAACATGGAGGCCAAGAAGCTGTCCAAGGACCGGATGAAGAAATACATGGCAAGAAGGAAGTGGCAGAAAACGGGCAATGCTGTGAGAGCCATCGGGAGACTGTCCTCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCTTCAACAGGGTCACCAACCAGCCCAATCAATGCGGAGAAACTAGAGTCTGAAGACGATGTCTCCCAAGCTTTCCTGGAGGCTGTTGCTGAGGAAAAGCCCCATGTGAAACCTTATTTCTCTAAGACCATCCGTGATCTGGAGGTCGTGGAGGGAAGCGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCTGAGGTGGTCTGGTTCAAGGATGACCAATCAATCAGGGAGTCCCGCCACTTCCAGATAGACTACGACGAAGACGGTAACTGCTCGCTCATCATCAGCGACGTCTGTGGGGATGATGACGCCAAGTACACCTGCAAGGCTGTCAACAGCCTCGGAGAAGCCACCTGCACTGCGGAGCTCATCGTGGAGACCATGGAGGAAGGGGAAGGGGAGGAGGGGGGAGAGGAGGAGGAGGAGGAGGAGGAGTGAGCGGCCGC

Amino Acid Sequence

MSGQLPGKTMGDVKLFASSHMSKTSHSVDPSKVSSMPLTEAPAFILPPRN
LCVKEGATAKFEGRVRGYPEPQVTWHRKGQAITNGGRFLLDCGVRGTFSL
VIHTVREEDKGKYTCEASNGSGARQVTVELTVEGNSMKKRDQPVLSKASG
FPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLK
GNVPLQPSARVSMSEKNGMQILEIRGVTRDDLGVYTCMVVNGSGKASMSA
ELSIPGLDNASRLAVRGTKAPSPDIRKEVTNGVSKDPETVAESKNCPSPQ
RSGSSARATNSHLKSPQEPKPKLCEDAPRKVPQSSILQKSTSTITLQALK
VQPEARVPAIGSFSPGEDRKSLAAPQQATLPTRQSSLGGSVGNKFVTGNI
PRESQRESTFPRFESQPQSQEVTEGQTVKFICEVSGIPKPDVGWFLEGIP
VRRREGITEVYEDGVSHHLCLLRARTRDSGRYSCTASNSLGQVSCSWSLL
VDRPNLAQTAPSFSSVLKDSVVIEGQDFVLRCSVQGTPAPRVTWLLNGQP
IQFAHSICEAGVAELHIQDALPEDRGTYTCLAENAMGQVSCSATVTVQEK
KGEGEREHRLSPARSKPIAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPE
VIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGE
VRTRAVLTVQEPHDGTQPWFISKPRSVTATLGQSVLISCAIAGDPFPTVH
WLRDGRALSKDSGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGEC
SCQVSLMLHNSPSRAPPRGREPASCEGLCGGGGVGAHGDGDRHGTLRPCW
PARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGK
KVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTISEEERKVHSPQQVDF
RSVLAKKGTPKTPVPEKAPPKAATPDFRSVLGGKKKSPSENGGNSAEVLN
VKAGESPTPAGDAQAIGALKPVGNAKPAETPKPIGNAKPTETLKPVGNTK
PAETLKPIANAQPSGSLKPVTNAQPAEPQKPVGNAKSAETSKPAGKEEVK
EVKNDVNCKKGQVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQC
QVISDPPATVTWSLNGKTLKTTKFIVLAQEGSRFSVSIEKALPEDRGLYK
CVAKNSAGQAECSCQVTVDDAQTSENTKAPEMKSRRPKSSLPPVLGTESD
ATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVAVTQPITC
KWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSR
QAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVE
IWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQES
ELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEER
LGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQEISIMNCLHHP
KLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQI
SEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLK
VLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNET
LANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWL
MKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSG
RKSSTGSPTSPINAEKLESEDDVSQAFLEAVAEEKPHVKPYFSKTIRDLE
VVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLII
SDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEEE
*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48883
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains a single amino acid at G1294V which requires repair. Also contains one known benign variation G261S (ensembl) along with 9 silent nucleotide changes T>C 297, T>C 567, T>C 630, A>G 1263, T>C 1689, G>A 1761, A>G 5145, A>G 5550 and C>T 5682 bp.