cDNA Clone SPAK

• Synonyms: STK39, DCHT, PASK
• Plasmid: pMA-RQ SPAK Nter GFP
• Parent Plasmid: pMA-RQ
• Genbank: NP_037365.2
• Species: Human

Sequence of Insert

GGCGTCTTGCAAAGGGACACCATGTAGGTATCCCGCTCGTCAACTTGTAAATGAACTTCAGGTTCTCTTTGGTGGCAGGAGAATTCTGAATGTGAGAACCTGGCGGCCGTTCAACGACCCCGCCCTCGGGGTCCCCAGGGGGCGTCCTCCGGCAAGCCTGATCGTCTGGGGCCAAGGCGCAGGGCGAGCAGGGAGGGAGGAGGAGAACCAGGGCCGGTGGCCCCGAAAGCACACACAAAGCGGCCTGACTCCTTTGGTGGGAACTTCCCCGGCCGCCAGCGGAGCGGGAGCGGGAGGGGGCGGCGGAGAGGGGGGGCGGCGGTTCCCCTCGCCGAGGCGGAGAACAGCGCGCGCTAGGCTCTGGGCGCGCGGCGAGCGCAGCGGCCGCCGCCTCCTCCCTTCCCCGCCTCCGCCGGCGCTGCCTCCTCCCTCCGCCCAGCTCGGCCTCCCTTGCTCCCTCCCCTCCTAGCTGGCTTCGGCGGGGACGGCGGCGGCGGCGGCGGGCGGGGAGGGCGTGCGCCGGCCGAGAGGTGTCGGCGGCGAGGCAAAGGAAGTTTCAAGTGGAAGGTCGTCCGTCGGCCGGCGCGTCCTCCTGCTCTCCTCCGCAGCATCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGCtGAaCCatctGGCTCGCCtGTcCAtGTgCAatTaCCaCAaCAaGCGGCCCCaGTGACAGCGGCGGCGGCGGCGGCCCCGGCGGCCGCGACAGCAGCGCCGGCCCCGGCAGCTCCCGCGGCCCCGGCCCCGGCCCCGGCCCCGGCGGCACAGGCTGTCGGCTGGCCCATCTGCAGGGACGCGTACGAGCTGCAGGAGGTTATCGGTCAGTGCGGCGGGCGCGGCGGGGCCGGCACAGGCCGGGCGCCGAGGCCGGGCGGGAGGGGGCGCGGGATGCTGGGCTAGCCTGCATGCACGCCCTGGAGGCAGAGAGGCCGGCTTCGGGGCCTACTGGCTTCAACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTTAATGTATTTGTTCGTTCTATGAGCGTTGGTGCTGCAGGAGGCGGCGCGGCGGATGTGACTCGCACTGCAGACGAGCCGCATGCTGCAAACTTTTATCTCAGGGATCGCCGGGGCGGTGGAGCGGAGGACCAGGCGGCGCCGCCGCATCTTCCTCCCCCCGCGTGCCGGGACCGCAGCCCGTGGCCGGTGGCCACGCGGACAGGCAGCCGCGGGTGGGGTGTGGTTGCGGGGGCCCCGCCCTGGGTTTCCAGGGCCCCGGGGGCCGCGTCCTGGGGGACTCTGC

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48744
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor plasmid designed to introduce an in-frame GFP to the N-terminus of SPAK. To be co-transfected into a TREX line expressing wild-type Cas9 with a guide plasmid; either 48606, 48607 or 48608. FACs sorting for GFP will enable quick identification of positives.