cDNA Clone Tof2

• Plasmid: pSC-B TOF2
• Parent Plasmid: pSC-B
• Genbank: NP_012935.3
• Species: Yeast

Sequence of Insert

GAATTCGCCCTTATGATAAAAATGTGGAGGTTACAGATTGTACTGGTGCCGCCAAGTGCTCAAGATATTATCACATTTCTCGAAGCACGCTTGAATACTCCACAATCAGTGTCTCCTATGGTCCAGTATAATGAAGATATAATTACGCACAACAACAGTATCAATAACTGTAGCGACCCCTCTCCTACGAGCCCGTCTTCGCAAAATTCTATTCAGTCGAATCGTAGTAGTGATTTCATTAACTACCTCCCGAATTGTAAAAAGTTTTTACATTTTACGGACGGTGACAATACCCTTTTACAACTATCTAATGAAATTTTAACTAAATTTGATAGACTTTATCCAAATTTCAAAGAATCCATTGAAATCGTATCTCTACAAGATAGGCATGGTTGTGATTTGGACTCTGAATTTATAATAAAAGACGTATTTGAAAATGATGGTGTGGTTTTGGTAATTTTAAAAGATGAATTAGACTGGAGTAGAAATCAACATATTTCACTTTTACAATTGGCAAGACAGCGGAGACGGCAGGACAACAAGCCATCCACAAAATCCATCGTTACTGAAAAGAGAAAAAAGATCTCTAAGGAAGACTTATCTTCTATATCAAATAAAGATACAATGCATCTAATAGCTAAAAGCTCGCTCAAGAATAACTTCATTAATAAATCAAGAGTATCAACGCCGCTAATGAACGAAATTCTACCGCTTGCAAGCAAGTATGATGCACTGAATAAAGAAAAGTGTCCCATGCCACTTACTTCCACAGTAGTGGCATCAAATGTACACAAAGACGTAAAAGATCACGCTCGTGCTAAAGAGGGAGTTGTAACGCAAGGGAGTGATAACAATAAAGAAAATATACCTTCATCCACTCAACAACAGAAAAATGATGGCGCTAAAAGGGCCGAATCAAAGGATTTAGATCTTTTACGCAATTCGTCAGAAGATGCTGATTATGAACCCGCAGATGAAAACTCCCCTCAAATTTCGTTTGATTCGATTGATACCGACTTCCAATTGAGCACTACTTCTCATACTAATTCTGATATGCATATACAATATTCTAACCCCTCTAGTGGTGCTCATAGTCCCAGGAAGAGTTCCTTAGAAATTAAAGTTCAAAACAAAAAAGGTGATGATCTGCCATTGAACGACAAAGATATAGGTGAGAATTGTAGAAGGATAGAAGCATTTTCAGATGAAGAGGATTTTAATGAAACCGATAATGATCGTGCGGATTCGTTTATCAACAATTCTAAGAAGGCATCGATGGGATTTCGGGATATCAATTCCGACTTGGATAGCGTTTCATTCAACAGTGATATTGAAAATGCAGTACAAAGTACTCAAAGTACCAAAAATGTTGTTTCTCCTCCCTTTTTTCCTGAAAAAGAATTGAATAATCGGTTACATCAAAGTCAAGGTAAGGAAGCCCTATTTCGACTTGTTGAGAAGGAGTTTCCTGATAAATCATTGGGTGCTGCGTCATCTACTTCGCATGCAAAAGACGTAAAAATACAAGAAACTATTCGGAAACTAAATAGGTTCAAGCCAACTGGGGAAACTAAGGTCCAAAAAAGGAATAGTATTACGGAACCATATTACGGAAAATTTGGAATAATGAAGAAAGATAAACCCAAATCCATAACTTCAAAAGGGGTAAGTCTGGAAACAAAGCACTTTGATGATCCCAATACCATTATTTCCGGTGAGAAATTCGCAAAATTTGGTAAAATAAAAGTGAAACGAAAAACTGATGATGTTGGATCGAAAGTTATAGAGTTTAAAAGAAAAAGAAATATGGGGAATAGATCCCTAAAAGATATTTTTGCCAATGCTGGGAAACCGCCAAATGCAGCTTCGACCATAAAAGTAGTCAAATTAATGAGAGATCCTGTTGATAATAGTAAAGACAAAGTGGAGGCAACTTCTAATAGTACGGCTCAGGAACAAGAACAAGTCTCACCCAAGTTGCCAGTTATGAATAGTACTCCTGGAAAAAGAAAAAATGGCCAAGCGATACCGTCCAGTTTAGAAAGAACACCGCAGCTCAAGAAAGTCAAGGTTACCAGGTCGCATTCTTCACCTTCTTCTTCTTCTTCCATGTCACTTGAATCTTCATTGGATAGCTCTTCCTCAGATGATTCGGATGATGATTCTGATTCGAGGAACGTGCAAGTTAAAAAAATTAATTTCAAAACAAGTCATGGACCAGCTGGAAATTCTAATGGAAAACCAATGCTGGATGTGGATGACAATGAAATAAATACAAAGAAGTACCAAACACCTAAATATGTTGAAAGTGATGAAGACGACCAGTAAAAGGGCGAATTC

Amino Acid Sequence

MIKMWRLQIVLVPPSAQDIITFLEARLNTPQSVSPMVQYNEDIITHNNSINNCSDPSPTSPSSQNSIQSNRSSDFINYLPNCKKFLHFTDGDNTLLQLSNEILTKFDRLYPNFKESIEIVSLQDRHGCDLDSEFIIKDVFENDGVVLVILKDELDWSRNQHISLLQLARQRRRQDNKPSTKSIVTEKRKKISKEDLSSISNKDTMHLIAKSSLKNNFINKSRVSTPLMNEILPLASKYDALNKEKCPMPLTSTVVASNVHKDVKDHARAKEGVVTQGSDNNKENIPSSTQQQKNDGAKRAESKDLDLLRNSSEDADYEPADENSPQISFDSIDTDFQLSTTSHTNSDMHIQYSNPSSGAHSPRKSSLEIKVQNKKGDDLPLNDKDIGENCRRIEAFSDEEDFNETDNDRADSFINNSKKASMGFRDINSDLDSVSFNSDIENAVQSTQSTKNVVSPPFFPEKELNNRLHQSQGKEALFRLVEKEFPDKSLGAASSTSHAKDVKIQETIRKLNRFKPTGETKVQKRNSITEPYYGKFGIMKKDKPKSITSKGVSLETKHFDDPNTIISGEKFAKFGKIKVKRKTDDVGSKVIEFKRKRNMGNRSLKDIFANAGKPPNAASTIKVVKLMRDPVDNSKDKVEATSNSTAQEQEQVSPKLPVMNSTPGKRKNGQAIPSSLERTPQLKKVKVTRSHSSPSSSSSMSLESSLDSSSSDDSDDDSDSRNVQVKKINFKTSHGPAGNSNGKPMLDVDDNEINTKKYQTPKYVESDEDDQ*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU26070
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation at S661 to KO internal EcoR1 site. ; EcoR1 sites from pSC-B used to cut out