Synonyms
AA408164, Ash
Plasmid
pX335 GRB2m KO antisense
Parent Plasmid
pX335
Expressed
GRB2m KO antisense guide
Genbank
NM_008163.3
Species
Mouse
Vector Type
Other
Antibiotic
Amp
DU Number
DU48841
Unit Source
Location
PPU
MTA
Notes
CRISPR guide plasmid. Targets antisense strand in exon 3, to be co-transfected with sense guide 48844 to KO GRB2. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.