cDNA Clone GRB2 mouse

• Synonyms: AA408164, Ash
• Plasmid: pKN7 GRB2m KO sense
• Parent Plasmid: pKN7
• Expressed: GRB2m KO sense guide
• Genbank: NM_008163.3
• Species: Mouse

Sequence of Insert

GCAGAACTCAATGGGAAAGA

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU48844
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets sense strand in exon 3, to be co-transfected with antisense guide 48841 to KO GRB2. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.