cDNA Clone Tof2

• Plasmid: pGADT7 5GA linker TOF2
• Parent Plasmid: pGADT7
• Expressed: Gal4AD 5GA TOF2
• Genbank: NP_012935.3
• Tag: GAL4 DNA-AD
• Species: Yeast

Sequence of Insert

GAATTCGCCCTTATGATAAAAATGTGGAGGTTACAGATTGTACTGGTGCCGCCAAGTGCTCAAGATATTATCACATTTCTCGAAGCACGCTTGAATACTCCACAATCAGTGTCTCCTATGGTCCAGTATAATGAAGATATAATTACGCACAACAACAGTATCAATAACTGTAGCGACCCCTCTCCTACGAGCCCGTCTTCGCAAAATTCTATTCAGTCGAATCGTAGTAGTGATTTCATTAACTACCTCCCGAATTGTAAAAAGTTTTTACATTTTACGGACGGTGACAATACCCTTTTACAACTATCTAATGAAATTTTAACTAAATTTGATAGACTTTATCCAAATTTCAAAGAATCCATTGAAATCGTATCTCTACAAGATAGGCATGGTTGTGATTTGGACTCTGAATTTATAATAAAAGACGTATTTGAAAATGATGGTGTGGTTTTGGTAATTTTAAAAGATGAATTAGACTGGAGTAGAAATCAACATATTTCACTTTTACAATTGGCAAGACAGCGGAGACGGCAGGACAACAAGCCATCCACAAAATCCATCGTTACTGAAAAGAGAAAAAAGATCTCTAAGGAAGACTTATCTTCTATATCAAATAAAGATACAATGCATCTAATAGCTAAAAGCTCGCTCAAGAATAACTTCATTAATAAATCAAGAGTATCAACGCCGCTAATGAACGAAATTCTACCGCTTGCAAGCAAGTATGATGCACTGAATAAAGAAAAGTGTCCCATGCCACTTACTTCCACAGTAGTGGCATCAAATGTACACAAAGACGTAAAAGATCACGCTCGTGCTAAAGAGGGAGTTGTAACGCAAGGGAGTGATAACAATAAAGAAAATATACCTTCATCCACTCAACAACAGAAAAATGATGGCGCTAAAAGGGCCGAATCAAAGGATTTAGATCTTTTACGCAATTCGTCAGAAGATGCTGATTATGAACCCGCAGATGAAAACTCCCCTCAAATTTCGTTTGATTCGATTGATACCGACTTCCAATTGAGCACTACTTCTCATACTAATTCTGATATGCATATACAATATTCTAACCCCTCTAGTGGTGCTCATAGTCCCAGGAAGAGTTCCTTAGAAATTAAAGTTCAAAACAAAAAAGGTGATGATCTGCCATTGAACGACAAAGATATAGGTGAGAATTGTAGAAGGATAGAAGCATTTTCAGATGAAGAGGATTTTAATGAAACCGATAATGATCGTGCGGATTCGTTTATCAACAATTCTAAGAAGGCATCGATGGGATTTCGGGATATCAATTCCGACTTGGATAGCGTTTCATTCAACAGTGATATTGAAAATGCAGTACAAAGTACTCAAAGTACCAAAAATGTTGTTTCTCCTCCCTTTTTTCCTGAAAAAGAATTGAATAATCGGTTACATCAAAGTCAAGGTAAGGAAGCCCTATTTCGACTTGTTGAGAAGGAGTTTCCTGATAAATCATTGGGTGCTGCGTCATCTACTTCGCATGCAAAAGACGTAAAAATACAAGAAACTATTCGGAAACTAAATAGGTTCAAGCCAACTGGGGAAACTAAGGTCCAAAAAAGGAATAGTATTACGGAACCATATTACGGAAAATTTGGAATAATGAAGAAAGATAAACCCAAATCCATAACTTCAAAAGGGGTAAGTCTGGAAACAAAGCACTTTGATGATCCCAATACCATTATTTCCGGTGAGAAATTCGCAAAATTTGGTAAAATAAAAGTGAAACGAAAAACTGATGATGTTGGATCGAAAGTTATAGAGTTTAAAAGAAAAAGAAATATGGGGAATAGATCCCTAAAAGATATTTTTGCCAATGCTGGGAAACCGCCAAATGCAGCTTCGACCATAAAAGTAGTCAAATTAATGAGAGATCCTGTTGATAATAGTAAAGACAAAGTGGAGGCAACTTCTAATAGTACGGCTCAGGAACAAGAACAAGTCTCACCCAAGTTGCCAGTTATGAATAGTACTCCTGGAAAAAGAAAAAATGGCCAAGCGATACCGTCCAGTTTAGAAAGAACACCGCAGCTCAAGAAAGTCAAGGTTACCAGGTCGCATTCTTCACCTTCTTCTTCTTCTTCCATGTCACTTGAATCTTCATTGGATAGCTCTTCCTCAGATGATTCGGATGATGATTCTGATTCGAGGAACGTGCAAGTTAAAAAAATTAATTTCAAAACAAGTCATGGACCAGCTGGAAATTCTAATGGAAAACCAATGCTGGATGTGGATGACAATGAAATAAATACAAAGAAGTACCAAACACCTAAATATGTTGAAAGTGATGAAGACGACCAGTAAAAGGGCGAATTC

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAMEYPYDVPDYAHMAMEASEFALGAGAGAGAGAMIKMWRLQIVLVPPSAQDIITFLEARLNTPQSVSPMVQYNEDIITHNNSINNCSDPSPTSPSSQNSIQSNRSSDFINYLPNCKKFLHFTDGDNTLLQLSNEILTKFDRLYPNFKESIEIVSLQDRHGCDLDSEFIIKDVFENDGVVLVILKDELDWSRNQHISLLQLARQRRRQDNKPSTKSIVTEKRKKISKEDLSSISNKDTMHLIAKSSLKNNFINKSRVSTPLMNEILPLASKYDALNKEKCPMPLTSTVVASNVHKDVKDHARAKEGVVTQGSDNNKENIPSSTQQQKNDGAKRAESKDLDLLRNSSEDADYEPADENSPQISFDSIDTDFQLSTTSHTNSDMHIQYSNPSSGAHSPRKSSLEIKVQNKKGDDLPLNDKDIGENCRRIEAFSDEEDFNETDNDRADSFINNSKKASMGFRDINSDLDSVSFNSDIENAVQSTQSTKNVVSPPFFPEKELNNRLHQSQGKEALFRLVEKEFPDKSLGAASSTSHAKDVKIQETIRKLNRFKPTGETKVQKRNSITEPYYGKFGIMKKDKPKSITSKGVSLETKHFDDPNTIISGEKFAKFGKIKVKRKTDDVGSKVIEFKRKRNMGNRSLKDIFANAGKPPNAASTIKVVKLMRDPVDNSKDKVEATSNSTAQEQEQVSPKLPVMNSTPGKRKNGQAIPSSLERTPQLKKVKVTRSHSSPSSSSSMSLESSLDSSSSDDSDDDSDSRNVQVKKINFKTSHGPAGNSNGKPMLDVDDNEINTKKYQTPKYVESDEDDQ*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU26111
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation at S661 to KO internal EcoR1 site.