cDNA Clone ctf4

• Plasmid: pGADT7 HA 5xGA Ctf4 (461-927) L867A A871G A897G I901A
• Parent Plasmid: pGADT7
• Expressed: AD-HA-5xGA-Ctf4(461-927) L867A A871G A897G I901A
• Genbank: NP_015461.1
• Tag: GAL4 DNA-AD
• Species: Yeast

Sequence of Insert

GAATTCGGAGCTGGTGCAGGCGCTGGAGCGGGTGCCACTCATTCGTTTCCAATCAGTTTGGCAAACACAGGAAAATTTCGTTATATGCCTTTTTCTCCAG
CGGGAACACCTTTTGGCTTTACTGACAGGCGTTATTTGACAATGAATGAAGTGGGCTACGTATCTACTGTCAAGAATAGTGAGCAATACAGCATAACTGT
CTCTTTTTTTGATGTTGGACGTTTTAGAGAATACCATTTCGAGGACTTATTTGGCTACGATTTATGCTTCCTAAATGAAAAAGGCACTTTATTTGGCCAA
TCCAAAACTGGGCAGATACAATATAGGCCACACGATAGCATACATTCAAACTGGACCAAGATTATTCCTTTGCAAGCTGGTGAGAGAATAACAAGTGTGG
CAGCCACCCCGGTTCGCGTTATTGTTGGTACATCATTAGGCTATTTCAGAAGTTTCAATCAATTCGGGGTTCCATTTGCTGTTGAAAAGACATCCCCAAT
TGTAGCGCTTACTGCTCAGAATTATAGGGTTTTTTCAGTACATTATTCGCAGTTTCATGGCCTTTCATACTCTTTATCTGAATTGGGTACTTCTAGTAAA
AGGTACTATAAAAGAGAGTGTCCACTTCCAATGAGTTTACCAAACATTAATTCTGATATGAAAAAAGACGCAAATCTTGACTACTACAATTTTAATCCGA
TGGGCATCAAAAGTTTGTTCTTTTCAAGCTACGGAGATCCATGCATTTTTGGGTCCGACAACACGCTTCTATTGTTATCAAAGTGGAGATCACCAGAAGA
AAGTAAATGGCTCCCTATTCTAGATAGCAACATGGAAATATGGAAGATGTCAGGAGGGAAGGAAACGACAGATATACATGTCTGGCCCTTGGCTTTGGCG
TATGACACATTGAATTGTATCTTAGTTAAGGGCAAGCATATATGGCCCGAGTTTCCCCTTCCGTTGCCATCCGAAATGGAGATTAGAATGCCAGTATTTG
TTAAGAGTAAATTACTAGAGGAAAACAAAGCTATATTAAATAAAAAGAACGAAATTGGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAGACAAAGAAAT
ACAGATTCCTGTTTCTATGGCGGCGGAAGAAGAGTATCTGCGCAGCAAGGTTTTGTCAGAGCTGTTGACAGATACACTCGAAAATGACGGTGAAATGTAC
GGCAACGAAAATGAGGTATTGGCAGCATTGAACGGTGCATATGATAAGGCTTTGGCACGTTTATTTGGGTCTGCATGCTCAGACCAAAATGTTGAAAAGG
CTCTTTCGCTTGCTCATGAATTAAAACAAGATAGAGCACTTACTGAAGCCGTTAAGGAATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTAAAAAAATTAA
TAATATAAGGGAAGCTAGATATGAACAGCAATTGAAATAA

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAMEYPYDVPDYAHMAMEASEFGAGAGAGAGATHSFPISLANTGKFRYMPFSPA
GTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVA
ATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSPIVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPM
GIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWLPILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFV
KSKLLEENKAILNKKNEIGADTEAEEGEEDKEIQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALARLFGSACSDQNVEKA
LSLAHELKQDRALTEAVKESERAELPSLVKKINNIREARYEQQLK*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU51085
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Hi ; Can you please mutate an existing plasmid pGADT7-Ctf4(461-927) (ampicillin plasmid) with the following mutations L867A, A871G, A897G and I901A. We will provide the template plasmid and a vector file containing all the information. Many thanks; Fabrizio