cDNA Clone
CUL3

Synonyms
CUL-3, PHA2E
Plasmid
pBABED P U6 CUL3 ex4 KO antisense
Parent Plasmid
pBABED P U6
Expressed
CUL3 ex4 KO antisense guide
Genbank
NM_003590.4
Species
Human
Sequence of Insert
GTATTTTGTTGTACATACACA




Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU48909
Location
PPU
MTA
Notes
CRISPR guide plasmid. Targets antisense strand of exon 5, designed to KO CUL3 when co-transfected with antisense guide 48903. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.