Synonyms
BRPK, PARK6
Plasmid
pX335 PINK1 Cter KI antisense
Parent Plasmid
pBABED P U6
Expressed
PINK1 Cter KI antisense guide + Cas9 D10A
Genbank
NM_032409.2
Species
Human
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU48947
Unit Source
Location
PPU
MTA
Notes
CRISPR guide plasmid. Targets antisense strand of last coding exon, designed to KI and in-frame YFP reporter when co-transfected with sense guide 48933 and donor 48992. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.