cDNA Clone
USP30

Plasmid
pBABED P U6 USP30 Ex5 KO antisense
Parent Plasmid
pBABED P U6
Expressed
USP30 Ex5 KO antisense guide
Genbank
NM_032663.3
Species
Human
Sequence of Insert
gAATGTGTGACCCGAGGCTGG
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU48957
Location
PPU
MTA
Notes
CRISPR guide plasmid. Targets antisense strand of exon 5, designed to KO USP30 when co-transfected with sense guide 48922. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.