cDNA Clone USP30

• Plasmid: pBABED P U6 USP30 Ex5 KO antisense
• Parent Plasmid: pBABED P U6
• Expressed: USP30 Ex5 KO antisense guide
• Genbank: NM_032663.3
• Species: Human

Sequence of Insert

gAATGTGTGACCCGAGGCTGG

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48957
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets antisense strand of exon 5, designed to KO USP30 when co-transfected with sense guide 48922. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.