cDNA Clone USP30

• Plasmid: pX335 USP30 Ex7 KO sense
• Parent Plasmid: pX335
• Expressed: USP30 Ex7 KO sense guide + Cas9 D10A
• Genbank: NM_032663.3
• Species: Human

Sequence of Insert

gTGGAAGACTCACTAGTAATA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48958
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets sense strand of exon 7, designed to KO USP30 when co-transfected with antisense guide 48923. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.