cDNA Clone RAB8B

• Plasmid: pMK-RQ RAB8B Nter HA donor
• Parent Plasmid: pMK-RQ
• Genbank: NM_016530.2
• Species: Human

Sequence of Insert

gcggccgcAAGGAAAGAAAGCATCTCTGCTCCAGGTCTCGGCGGAAGAGATGCGGGAACTACGGGGCTCTGATTCTGAGGTCCTGGATTTGGAAGGCGTCTGCACACAAAGAAATAACAAAGTTAAGTTCCCGCGGCTCGACCAGCAGAGTACGAAACGGGGCCTCCCTTCCCAGCTCGGGTGGAAACGCCTCCACCCTGTCACCCCGCCCATAGCAGGTCTTTTATCCGCTGGAGCCGCCTGGACAGTCCTCGCGGGGCACGCCCGAGGCTCCCCGGAGGAACTACAAGTCCCATGAAGTCCCGGGGCGCGGGCGGTGATGACGAAGCCCTCGCCGATTGGCCGCACGGGGCGGGGCGCGCAGGCCTTCTCCGAGAGGAGGGGCGGGGCGTGGGGCGGGGCGAGCAGCGGCGGATTGGCGGGCACGCCCCCTCGCCCGCGGCCCCCTCCCCGCCTCTCTCCACCGCCTCCTCTGGCTCCCCGGTCAGAGGGCCGGAGCGAGAAGATGTACCCATACGATGTGCCAGATTACGCCGCGAAGACGTACGATTATCTCTTCAAGCTCCTGttaATCGGtGACagcGGGGTAGGCAAGACCTGCCTCCTGTTCCGCTTCTCAGAGGACGCCTTCAACACCACCTTCATCTCCACCATCGGTGAGGGAGGGGCCGCGGCCCGGGACCGGGTAGAGAATTGGGGGGCGGGTACTAGGGGACGGGGAAAGGGCGGGGGGCGCTTGGGAGCCCGGGAGAGGAGACCCCGGGGACTGCAAGGTGGCGGGGGCACTGAGAGGGGCGAGGGCGTGAGGGAGAGGGGCGAGGGCGTGAGGGAGAGAGGCGAGCGGGAACGTGAGGCTAAGGGCGAAGGGGGAACTTGGGGGCAAAGGAACGGGAAGGGGAACAGAGGAACAAGGACTAGGGGTATTGGGGGAACGAGAGGGTTCGGAGACTGACAGGGAGGGGTCTTTGAGGGACAAAATGTGTTGGGGGGAAGACTCAGGAAATCTCATGTAGAGAGAAGGGGATAGTGGACGTGCCAATGATTAAAAGACAGGAGAGGAAGGAAATGTGAAGGGGGAAATGAGcggccgc

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU48994
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor plasmid. Designed to KI an HA tag to the N-terminus of RAB8B when co-transfected with guides 48935 and 48949. Donor sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line, contains several silent mutations to prevent binding of guides.