cDNA Clone USP32

• Synonyms: NY-REN-60; USP10
• Plasmid: pcDNA5 FRT/TO-GFP USP32 C743A
• Parent Plasmid: pcDNA FRT TO GFP
• Expressed: GFP USP32 C743A
• Genbank: NM_032582.3 GI:44889413
• Tag: GFP
• Species: Human

Sequence of Insert

ctcgagATGGGTGCCAAGGAGTCACGGATCGGATTCCTCAGCTACGAGGA
GGCGCTGAGGAGAGTTACAGATGTAGAGCTAAAACGACTGAAGGATGCTT
TCAAGAGGACCTGTGGACTCTCATATTACATGGGCCAGCACTGCTTCATC
CGGGAAGTGCTTGGGGATGGAGTGCCTCCAAAGGTTGCTGAGGTGATTTA
CTGTTCTTTTGGTGGAACATCCAAAGGGCTGCACTTCAATAATTTAATAG
TTGGACTTGTCCTCCTTACAAGAGGCAAAGATGAAGAGAAAGCAAAATAC
ATTTTTAGTCTTTTTTCAAGTGAATCTGGGAACTATGTTATACGGGAAGA
AATGGAAAGAATGCTCCACGTGGTGGATGGTAAAGTCCCAGATACACTCA
GGAAGTGTTTCTCAGAGGGTGAAAAGGTAAACTATGAAAAGTTTAGAAAT
TGGCTTTTTCTAAACAAAGATGCTTTTACTTTCTCTCGATGGCTTCTATC
TGGAGGTGTGTATGTTACCCTCACTGATGATAGTGATACTCCTACTTTCT
ACCAAACTCTGGCTGGAGTCACACATTTGGAGGAATCAGACATCATTGAT
CTTGAGAAACGCTATTGGTTATTGAAGGCTCAATCCCGGACTGGACGATT
TGATTTAGAGACATTTGGCCCATTGGTTTCACCACCTATTCGTCCATCTC
TAAGTGAAGGTTTGTTTAATGCTTTTGATGAAAATCGTGACAATCACATA
GATTTTAAGGAGATATCCTGTGGGTTATCAGCCTGTTGCAGGGGACCCCT
GGCTGAAAGACAAAAATTTTGCTTCAAGGTATTTGATGTTGACCGTGATG
GAGTTCTCTCCAGGGTTGAACTGAGAGACATGGTGGTTGCACTTTTAGAA
GTCTGGAAGGACAACCGCACTGATGATATTCCTGAATTACATATGGATCT
CTCTGATATTGTAGAAGGCATACTGAATGCACATGACACCACAAAGATGG
GTCATCTTACTCTGGAAGACTATCAGATCTGGAGTGTGAAAAATGTTCTT
GCCAATGAGTTTTTGAACCTCCTTTTCCAGGTGTGTCACATAGTTCTGGG
GTTAAGACCAGCTACTCCGGAAGAAGAAGGACAAATTATTAGAGGATGGT
TAGAACGAGAGAGCAGGTATGGTCTGCAAGCAGGACACAACTGGTTTATC
ATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATACGATGC
CAACCCTGTGGTAATTGAGCCATCATCTGTTTTGAATGGAGGAAAATACT
CATTTGGAACTGCAGCCCATCCTATGGAGCAGGTCGAAGATAGAATTGGA
AGCAGCCTCAGTTACGTGAATACTACAGAAGAGAAATTTTCAGACAACAT
TTCTACTGCATCTGAAGCCTCAGAAACTGCTGGCAGCGGCTTTCTGTATT
CTGCCACACCAGGGGCAGATGTTTGCTTTGCTCGACAACATAACACTTCT
GACAATAACAACCAGTGTTTGCTGGGAGCCAATGGGAATATTTTGTTGCA
CCTTAACCCTCAGAAACCAGGGGCTATTGATAATCAGCCATTAGTAACTC
AAGAACCAGTAAAGGCTACATCATTAACACTAGAAGGAGGACGATTAAAA
CGAACTCCACAGCTGATTCATGGAAGAGACTATGAAATGGTCCCAGAACC
TGTGTGGAGAGCACTTTATCACTGGTATGGAGCAAACCTGGCCTTACCTA
GACCAGTTATCAAGAACAGCAAGACAGACATCCCAGAGCTGGAATTATTT
CCCCGCTATCTTCTCTTCCTGAGACAGCAGCCTGCCACTCGGACACAGCA
GTCTAACATCTGGGTGAATATGGGAAATGTACCTTCTCCGAATGCACCTT
TAAAGCGGGTATTAGCCTATACAGGCTGTTTTAGTCGAATGCAGACCATC
AAGGAAATTCACGAATATCTATCTCAAAGGCTGCGCATTAAAGAGGAAGA
TATGCGCCTGTGGCTATACAACAGTGAGAACTACCTTACTCTTCTGGATG
ATGAGGATCATAAATTGGAATATTTGAAAATCCAGGATGAACAACACCTG
GTAATTGAAGTTCGCAACAAAGATATGAGTTGGCCTGAGGAGATGTCTTT
TATAGCAAATAGTAGTAAAATAGATAGACACAAGGTTCCCACAGAAAAGG
GAGCCACAGGTCTAAGCAATCTGGGAAACACAgcCTTCATGAACTCAAGC
ATCCAGTGTGTTAGTAACACACAGCCACTGACACAGTATTTTATCTCAGG
GAGACATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAAGGGGC
ATATGGCTAAATGCTATGGTGATTTAGTGCAGGAACTTTGGAGTGGAACT
CAGAAGAATGTTGCCCCATTAAAGCTTCGGTGGACCATAGCAAAATATGC
TCCCAGGTTTAATGGGTTTCAGCAACAGGACTCCCAAGAACTTCTGGCTT
TTCTCTTGGATGGTCTTCATGAAGATCTTAATCGAGTCCATGAAAAGCCA
TATGTGGAACTGAAGGACAGTGATGGGCGACCAGACTGGGAAGTAGCTGC
AGAGGCCTGGGACAACCATCTAAGAAGAAATAGATCAATTGTTGTGGATT
TGTTCCATGGGCAGCTAAGATCTCAAGTAAAATGCAAGACATGTGGGCAT
ATAAGTGTCCGATTTGACCCTTTCAATTTTTTGTCTTTGCCACTACCAAT
GGACAGTTATATGCACTTAGAAATAACAGTGATTAAGTTAGATGGTACTA
CCCCTGTACGGTATGGACTAAGACTGAATATGGATGAAAAGTACACAGGT
TTAAAAAAACAGCTGAGTGATCTCTGTGGACTTAATTCAGAACAAATCCT
TCTAGCAGAAGTACATGGTTCCAACATAAAGAACTTTCCTCAGGACAACC
AAAAAGTACGACTCTCAGTGAGTGGATTTTTGTGTGCATTTGAAATTCCT
GTCCCTGTGTCTCCAATTTCAGCTTCTAGTCCAACACAGACAGATTTCTC
CTCTTCGCCATCTACAAATGAAATGTTCACCCTAACTACCAATGGGGACC
TACCCCGACCAATATTCATCCCCAATGGAATGCCAAACACTGTTGTGCCA
TGTGGAACTGAGAAGAACTTCACAAATGGAATGGTTAATGGTCACATGCC
ATCTCTTCCTGACAGCCCCTTTACAGGTTACATCATTGCAGTCCACCGAA
AAATGATGAGGACAGAACTGTATTTCCTGTCATCTCAGAAGAATCGCCCC
AGCCTCTTTGGAATGCCATTGATTGTTCCATGTACTGTGCATACCCGGAA
GAAAGACCTATATGATGCGGTTTGGATTCAAGTATCCCGGTTAGCGAGCC
CACTCCCACCTCAGGAAGCTAGTAATCATGCCCAGGATTGTGACGACAGT
ATGGGCTATCAATATCCATTCACTCTACGAGTTGTGCAGAAAGATGGGAA
CTCCTGTGCTTGGTGCCCATGGTATAGATTTTGCAGAGGCTGTAAAATTG
ATTGTGGGGAAGACAGAGCTTTCATTGGAAATGCCTATATCGCTGTGGAT
TGGGATCCCACAGCCCTTCACCTTCGCTATCAAACATCCCAGGAAAGGGT
TGTAGATGAGCATGAGAGTGTGGAGCAGAGTCGGCGAGCGCAAGCCGAGC
CCATCAACCTGGACAGCTGTCTCCGTGCTTTCACCAGTGAGGAAGAGCTA
GGGGAAAATGAGATGTACTACTGTTCCAAGTGTAAGACCCACTGCTTAGC
AACAAAGAAGCTGGATCTCTGGAGGCTTCCACCCATCCTGATTATTCACC
TTAAGCGATTTCAATTTGTAAATGGTCGGTGGATAAAATCACAGAAAATT
GTCAAATTTCCTCGGGAAAGTTTTGATCCAAGTGCTTTTTTGGTACCAAG
AGACCCGGCTCTCTGCCAGCATAAACCACTCACACCCCAGGGGGATGAGC
TCTCTGAGCCCAGGATTCTGGCAAGGGAGGTGAAGAAAGTGGATGCGCAG
AGTTCGGCTGGGGAAGAGGACGTGCTCCTGAGCAAAAGCCCATCCTCACT
CAGCGCTAACATCATCAGCAGCCCGAAAGGTTCTCCTTCTTCATCAAGAA
AAAGTGGAACCAGCTGTCCCTCCAGCAAAAACAGCAGCCCTAATAGCAGC
CCACGGACTTTGGGGAGGAGCAAAGGGAGGCTCCGGCTGCCCCAGATTGG
CAGCAAAAATAAACTGTCAAGTAGTAAAGAGAACTTGGATGCCAGCAAAG
AAAATGGGGCTGGGCAGATATGTGAGCTGGCTGACGCCTTGAGTCGAGGG
CATGTGCTGGGGGGCAGCCAACCAGAGTTGGTCACTCCTCAGGACCATGA
GGTAGCTTTGGCCAATGGATTCCTTTATGAGCATGAAGCATGTGGCAATG
GCTACAGCAATGGTCAGCTTGGAAACCACAGTGAAGAAGACAGCACTGAT
GACCAAAGAGAAGATACTCGTATTAAGCCTATTTATAATCTATATGCAAT
TTCGTGCCATTCAGGAATTCTGGGTGGGGGCCATTACGTCACTTATGCCA
AAAACCCAAACTGCAAGTGGTACTGTTACAATGACAGCAGCTGTAAGGAA
CTTCACCCGGATGAAATTGACACCGACTCTGCCTACATTCTTTTCTATGA
GCAGCAGGGGATAGACTATGCACAATTTCTGCCAAAGACTGATGGCAAAA
AGATGGCAGACACAAGCAGTATGGATGAAGACTTTGAGTCTGATTACAAA
AAGTACTGTGTGTTACAGTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFAS
LEMGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMGQHCFI
REVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVGLVLLTRGKDEEKAKY
IFSLFSSESGNYVIREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRN
WLFLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIID
LEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHI
DFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELRDMVVALLE
VWKDNRTDDIPELHMDLSDIVEGILNAHDTTKMGHLTLEDYQIWSVKNVL
ANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQAGHNWFI
ISMQWWQQWKEYVKYDANPVVIEPSSVLNGGKYSFGTAAHPMEQVEDRIG
SSLSYVNTTEEKFSDNISTASEASETAGSGFLYSATPGADVCFARQHNTS
DNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLK
RTPQLIHGRDYEMVPEPVWRALYHWYGANLALPRPVIKNSKTDIPELELF
PRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMQTI
KEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHKLEYLKIQDEQHL
VIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTAFMNSS
IQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGT
QKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP
YVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGH
ISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLNMDEKYTG
LKKQLSDLCGLNSEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIP
VPVSPISASSPTQTDFSSSPSTNEMFTLTTNGDLPRPIFIPNGMPNTVVP
CGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSSQKNRP
SLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDS
MGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNAYIAVD
WDPTALHLRYQTSQERVVDEHESVEQSRRAQAEPINLDSCLRAFTSEEEL
GENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKI
VKFPRESFDPSAFLVPRDPALCQHKPLTPQGDELSEPRILAREVKKVDAQ
SSAGEEDVLLSKSPSSLSANIISSPKGSPSSSRKSGTSCPSSKNSSPNSS
PRTLGRSKGRLRLPQIGSKNKLSSSKENLDASKENGAGQICELADALSRG
HVLGGSQPELVTPQDHEVALANGFLYEHEACGNGYSNGQLGNHSEEDSTD
DQREDTRIKPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYCYNDSSCKE
LHPDEIDTDSAYILFYEQQGIDYAQFLPKTDGKKMADTSSMDEDFESDYK
KYCVLQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24864
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41455