cDNA Clone MYLK (mouse)

• Synonyms: AW489456, KRP, MLCK108, MLCK210, Mlck, nmMlck, smMLCK
• Plasmid: pCMV5D FLAG MYLKm
• Parent Plasmid: pCMV5D FLAG
• Expressed: FLAG-MYLKm
• Genbank: NM_139300.3
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GTCGACGCCATGTCTGGCCAGTTACCTGGGAAGACCATGGGGGACGTGAA
ACTGTTTGCCTCGTCACACATGTCTAAAACCTCCCACAGTGTGGATCCCT
CGAAGGTCAGCTCCATGCCTCTCACCGAGGCCCCAGCTTTCATTCTGCCC
CCTCGGAACCTCTGCGTCAAAGAAGGAGCCACAGCCAAGTTCGAAGGGAG
GGTCAGGGGCTACCCAGAGCCCCAGGTGACATGGCACAGAAAAGGGCAAG
CCATCACCAATGGTGGCCGCTTCCTGCTGGACTGTGGAGTGCGGGGGACT
TTTAGCCTTGTCATCCACACTGTCCGCGAGGAGGACAAGGGCAAATACAC
CTGTGAAGCTAGCAATGGCAGTGGCGCCCGCCAGGTGACCGTGGAGCTGA
CTGTGGAAGGGAATTCTATGAAGAAGCGTGATCAGCCTGTTCTTTCTAAA
GCCTCAGGATTTCCAGGGGAGACTCGTCCTAGCATCTGGGGTGAGTGCCC
ACCAAAGTTTGCCACCAAGCTGGGCAGAGCTGTGGTCAAAGAAGGGCAGA
TGGGGCGATTCTCCTGCAAGATCACCGGCCGGCCTCCACCACAGGTCACC
TGGCTCAAGGGAAATGTCCCGTTGCAGCCAAGTGCCCGCGTGTCAATGTC
AGAGAAGAATGGCATGCAGATCCTGGAGATCCGTGGGGTCACTCGGGATG
ACCTGGGCGTGTACACCTGCATGGTGGTGAATGGGTCAGGGAAAGCCTCC
ATGTCGGCGGAGCTCTCCATCCCAGGTTTGGACAATGCCTCTAGGTTGGC
TGTGAGAGGAACCAAAGCCCCCAGTCCAGACATCAGGAAGGAGGTGACCA
ATGGAGTCTCCAAAGACCCAGAGACTGTGGCTGAAAGTAAGAACTGTCCA
AGTCCCCAGAGGAGTGGCTCCTCAGCCCGGGCCACCAACAGTCACCTCAA
GTCCCCGCAGGAGCCCAAGCCAAAGCTGTGTGAGGATGCTCCCAGAAAGG
TCCCGCAGTCCTCCATCCTGCAGAAGTCCACCAGCACCATCACTCTGCAG
GCCTTGAAAGTCCAGCCAGAAGCGAGAGTACCAGCCATAGGGTCCTTCTC
TCCTGGGGAAGACAGGAAGAGTCTGGCTGCCCCTCAGCAAGCCACACTCC
CTACCAGACAGTCTAGCCTGGGAGGTTCCGTTGGGAACAAGTTTGTTACC
GGGAACATCCCCAGAGAGAGCCAAAGGGAGTCAACATTCCCCAGATTTGA
GAGTCAGCCCCAAAGCCAGGAGGTCACTGAAGGTCAGACAGTCAAGTTCA
TCTGCGAGGTTTCAGGTATACCAAAGCCTGACGTGGGCTGGTTCCTGGAA
GGCATCCCCGTGAGGAGACGGGAAGGCATCACTGAGGTTTATGAAGATGG
TGTATCCCATCACCTGTGCCTGCTGAGAGCCCGGACGAGAGACAGTGGGA
GGTACAGCTGCACAGCATCCAATAGCCTTGGCCAGGTGTCCTGCAGCTGG
AGCCTGCTGGTGGACCGGCCGAACTTGGCACAGACAGCCCCTTCATTCTC
TAGTGTTCTGAAGGACAGTGTTGTCATTGAGGGCCAGGATTTTGTGCTAC
GGTGCTCAGTGCAGGGGACTCCAGCACCCCGGGTCACGTGGCTGCTCAAT
GGACAGCCCATCCAGTTTGCTCACTCCATCTGTGAGGCAGGCGTGGCCGA
GCTCCACATCCAGGATGCCCTGCCAGAGGACCGTGGCACCTACACCTGCC
TGGCTGAGAATGCCATGGGACAGGTGTCCTGCAGCGCCACTGTCACCGTC
CAAGAGAAGAAAGGTGAAGGAGAGAGGGAACACCGTCTGTCCCCAGCTCG
CAGCAAGCCCATCGCACCCATCTTCCTGCAGGGCCTCTCCGACCTCAAAG
TCATGGATGGAAGCCAGGTCACTATGACAGTCCAGGTGTCAGGGAACCCA
CCTCCGGAAGTCATCTGGCTTCACGACGGGAATGAGATTCAGGAGTCCGA
GGACTTCCACTTCGAGCAGAAGGGTGGCTGGCACAGCCTGTGTATCCAGG
AGGTGTTCCCGGAGGACACGGGCACGTACACCTGTGAGGCCTGGAACAGT
GCCGGGGAGGTCCGCACCCGGGCCGTCCTCACCGTGCAAGAGCCTCATGA
TGGCACCCAGCCCTGGTTCATCAGCAAGCCTCGTTCGGTGACAGCCACCC
TGGGCCAGAGTGTCCTCATCTCCTGCGCCATAGCTGGAGACCCCTTTCCC
ACCGTGCACTGGCTCCGGGATGGCAGAGCCCTCTCCAAGGACAGTGGCCA
CTTTGAGCTGCTCCAGAATGAGGACGTGTTCACCCTGGTTCTAAAGAACG
TGCAGCCCTGGCATGCCGGCCAGTATGAGATTCTGCTCAAGAACCGGGTT
GGCGAGTGTAGTTGCCAAGTGTCACTGATGCTTCACAACAGCCCTTCCAG
AGCCCCACCACGGGGGCGGGAGCCTGCCAGCTGCGAGGGCCTCTGTGGTG
GTGGAGGAGTTGGTGCTCATGGTGATGGTGACCGTCATGGGACCCTGAGG
CCCTGCTGGCCAGCAAGAGGGCAAGGTTGGCCTGAGGAGGAAGACGGAGA
AGACGTGAGGGGGCTGTTGAAGAGACGGGTGGAGACCAGGCTGCACACAG
AAGAGGCCATCCGCCAGCAGGAGGTGGGGCAGCTGGATTTTCGAGACCTC
CTGGGAAAGAAGGTGAGCACCAAGACCGTCTCAGAAGACGACCTGAAGGA
CATCCCAGCCGAGCAGATGGATTTCCGCGCCAACCTTCAGCGGCAAGTGA
AGCCAAAGACCATATCCGAGGAAGAGAGGAAGGTGCATAGCCCTCAGCAG
GTTGACTTCCGGTCTGTCCTCGCCAAGAAGGGGACCCCTAAGACCCCCGT
TCCTGAGAAGGCACCTCCAAAAGCTGCCACCCCAGACTTTCGCTCTGTGC
TAGGTGGCAAGAAGAAGTCACCCTCGGAGAATGGGGGCAACAGCGCTGAG
GTCTTGAATGTCAAGGCTGGGGAAAGCCCCACACCTGCAGGCGATGCGCA
GGCCATCGGGGCCCTGAAACCCGTAGGCAATGCCAAGCCTGCTGAGACCC
CGAAACCTATAGGTAATGCCAAGCCTACCGAGACCCTGAAGCCTGTGGGC
AACACCAAGCCTGCCGAGACCCTGAAACCCATAGCCAACGCACAGCCTTC
AGGGTCCCTGAAACCTGTAACCAACGCACAACCTGCTGAGCCCCAGAAGC
CTGTGGGCAATGCCAAGTCTGCTGAGACCTCGAAACCAGCTGGGAAAGAA
GAAGTCAAGGAGGTAAAGAATGATGTGAACTGTAAGAAAGGGCAGGTCGG
AGCCACGGGCAATGAAAAGCGACCCGAGAGCCAAGGCTCGGCCCCAGTTT
TCAAGGAAAAGCTACAAGATGTCCATGTGGCAGAGGGGGAGAAGCTGCTA
CTCCAATGCCAGGTGATCTCTGATCCCCCCGCCACTGTCACCTGGTCGCT
GAATGGGAAGACACTCAAGACCACAAAATTCATCGTCCTCGCCCAAGAAG
GCTCACGTTTCTCTGTCTCCATCGAGAAGGCACTGCCTGAGGACCGAGGT
TTGTACAAGTGTGTAGCCAAGAACAGCGCTGGCCAGGCAGAGTGTTCCTG
CCAAGTCACTGTGGACGATGCTCAGACCAGTGAGAACACCAAGGCCCCGG
AGATGAAATCCCGGAGACCCAAGAGCTCTCTTCCCCCTGTGCTAGGAACA
GAGAGCGACGCAACTGTGAAAAAGAAACCTGCCCCCAAGACACCCACAAA
AGCAGCTATGCCCCCTCAAATCATCCAGTTCCCCGAGGACCAGAAGGTGC
GCGCAGGAGAACCTGTGGAGCTGTTTGGGAAAGTGGCTGGCACCCAGCCC
ATCACCTGCAAGTGGATGAAGTTCCGAAAGCAGATCCAGGAGAGCGAGCA
TATCAAGGTGGAGAATGGCGAGAGCGGCAGCAAGCTCACCATCCTGGCCG
CGCGGCAGGAGCACTGTGGCTGCTACACACTGGTGGTGGAGAACAAGCTG
GGCAGCAGGCAGGCCCAAGTCAACCTCACGGTCGTGGATAAGCCAGACCC
CCCGGCTGGCACACCTTGTGCTTCTGACATTCGGAGTTCCTCACTGACCC
TGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTAC
AATGTGGAGATCTGGGACACAGAGGACAAGGTGTGGAAAGAGCTAGCCAC
GTGCCGCAGCACCTCTTTTAATGTCCAAGACCTGCTGCCTGACCGCGAGT
ACAAATTCCGAGTGCGTGCAGTCAACGTCTATGGAACCAGCGAGCCAAGC
CAAGAGTCCGAGCTCACAGCTGTGGGAGAGAAACCTGAAGAGCCAAAGGA
TGAAGTGGAAGTGTCCGATGATGACGAAAAGGAACCTGAGGTTGATTACC
GGACGGTGACTGTTAACACTGAACAGAAAGTGTCTGATGTGTATGACATT
GAAGAGCGACTAGGATCTGGGAAGTTTGGACAAGTCTTCCGACTTGTGGA
AAAGAAAACTGGAAAAATCTGGGCAGGGAAATTTTTCAAGGCCTATTCCG
CCAAGGAGAAAGACAACATCCGTCAGGAGATCAGCATCATGAACTGCCTC
CACCACCCCAAGCTGGTCCAGTGTGTGGATGCCTTCGAAGAAAAGGCCAA
CATTGTCATGGTTCTGGAGATCGTGTCCGGGGGTGAGCTGTTTGAGCGTA
TCATTGACGAGGACTTCGAGCTGACGGAGCGGGAGTGTATCAAGTACATG
AGGCAGATCTCGGAAGGGGTGGAGTACATCCACAAGCAGGGCATTGTGCA
CCTGGACCTCAAGCCCGAGAACATCATGTGTGTCAACAAGACAGGCACCA
GGATCAAGCTCATTGACTTCGGCCTGGCACGGAGACTCGAGAACGCTGGG
TCTCTGAAGGTCCTCTTTGGGACCCCAGAGTTTGTGGCTCCTGAAGTGAT
CAACTATGAGCCCATCGGCTACGCCACAGACATGTGGAGCATCGGGGTCA
TCTGCTATATTCTGGTCAGCGGACTTTCTCCCTTCATGGGTGACAATGAT
AACGAGACCTTAGCCAACGTCACTTCAGCCACCTGGGACTTCGATGACGA
GGCGTTCGATGAGATCTCCGATGACGCAAAGGATTTCATCAGTAACTTGC
TGAAGAAAGATATGAAAAACCGTCTGGACTGCACCCAGTGCCTTCAGCAC
CCATGGCTGATGAAAGACACCAAGAACATGGAGGCCAAGAAGCTGTCCAA
GGACCGGATGAAGAAATACATGGCAAGAAGGAAGTGGCAGAAAACGGGCA
ATGCTGTGAGAGCCATCGGGAGACTGTCCTCTATGGCAATGATCTCAGGG
CTCAGTGGCAGGAAATCTTCAACAGGGTCACCAACCAGCCCAATCAATGC
GGAGAAACTAGAGTCTGAAGACGATGTCTCCCAAGCTTTCCTGGAGGCTG
TTGCTGAGGAAAAGCCCCATGTGAAACCTTATTTCTCTAAGACCATCCGT
GATCTGGAGGTCGTGGAGGGAAGCGCTGCTAGATTTGACTGCAAGATTGA
AGGATACCCAGACCCTGAGGTGGTCTGGTTCAAGGATGACCAATCAATCA
GGGAGTCCCGCCACTTCCAGATAGACTACGACGAAGACGGTAACTGCTCG
CTCATCATCAGCGACGTCTGTGGGGATGATGACGCCAAGTACACCTGCAA
GGCTGTCAACAGCCTCGGAGAAGCCACCTGCACTGCGGAGCTCATCGTGG
AGACCATGGAGGAAGGGGAAGGGGAGGAGGGGGGAGAGGAGGAGGAGGAG
GAGGAGGAGTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSFEEFASLDAMSGQLPGKTMGDVKLFASSHMSKTSHSVDPSKVSSMPLTEAPAFILPPRNLCVKEGATAKFEGRVRGYPEPQVTWHRKGQ
AITNGGRFLLDCGVRGTFSLVIHTVREEDKGKYTCEASNGSGARQVTVELTVEGNSMKKRDQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQ
MGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIRGVTRDDLGVYTCMVVNGSGKASMSAELSIPGLDNASRLAVRGTKAPSPDIRKEVT
NGVSKDPETVAESKNCPSPQRSGSSARATNSHLKSPQEPKPKLCEDAPRKVPQSSILQKSTSTITLQALKVQPEARVPAIGSFSPGEDRKSLAAPQQATL
PTRQSSLGGSVGNKFVTGNIPRESQRESTFPRFESQPQSQEVTEGQTVKFICEVSGIPKPDVGWFLEGIPVRRREGITEVYEDGVSHHLCLLRARTRDSG
RYSCTASNSLGQVSCSWSLLVDRPNLAQTAPSFSSVLKDSVVIEGQDFVLRCSVQGTPAPRVTWLLNGQPIQFAHSICEAGVAELHIQDALPEDRGTYTC
LAENAMGQVSCSATVTVQEKKGEGEREHRLSPARSKPIAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQ
EVFPEDTGTYTCEAWNSAGEVRTRAVLTVQEPHDGTQPWFISKPRSVTATLGQSVLISCAIAGDPFPTVHWLRDGRALSKDSGHFELLQNEDVFTLVLKN
VQPWHAGQYEILLKNRVGECSCQVSLMLHNSPSRAPPRGREPASCEGLCGGGGVGAHGDGDRHGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHT
EEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTISEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKAPPKAATPDFRSV
LGGKKKSPSENGGNSAEVLNVKAGESPTPAGDAQAIGALKPVGNAKPAETPKPIGNAKPTETLKPVGNTKPAETLKPIANAQPSGSLKPVTNAQPAEPQK
PVGNAKSAETSKPAGKEEVKEVKNDVNCKKGQVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVISDPPATVTWSLNGKTLKTTKFIVLAQE
GSRFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAQTSENTKAPEMKSRRPKSSLPPVLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKV
RAGEPVELFGKVAGTQPITCKWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLT
LSWYGSSYDGGSAVQSYNVEIWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDY
RTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFER
IIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGV
ICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTG
NAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEDDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSI
RESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEEE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU52134
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains one known benign variation G261S (ensembl) along with 9 silent nucleotide changes T>C 297, T>C 567, T>C 630, A>G 1263, T>C 1689, G>A 1761, A>G 5145, A>G 5550 and C>T 5682 bp.; Some gaps remain in sequence though unlikely to differ from the source vector from which it was shuttled.; Difficult to work with, grow at 30oC to prevent rearrangement.