cDNA Clone Cul2 (xenopus)

• Synonyms: cullin2
• Plasmid: pET15 6HIS SUMO Cul2 1-383 (Xenopus) V340R L344D
• Parent Plasmid: pET 6HIS SUMO1
• Expressed: 6HIS SUMO Cul2 1-383 (Xenopus) V340R L344D
• Tag: SUMO
• Tag: 6His
• Species: Xenopus laevis

Sequence of Insert

ATGTCATTGAAGCCACGGGTAGTGGATTTTGATGAAACATGGAACAAACTTTTAACTACAATCAAAGCTGTTGTCATGCTGGATTATGTTGAAAGAGCAACATGGAATGACCGTTTCTCAGATATTTACGCACTGTGTGTTGCCTATCCAGAGCCTCTTGGAGAAAGATTGTATACAGAAACAAAGATATTTCTGGAAAACCATGTACAGCAGTTGCATACGAGAGTTCTGGATTCAGCAGAGCAAGTACTGGTAATGTACTTCAGATACTGGGAGGAATATAGCAGAGGAGCTGATTACATGGATTGTTTATATAGGTATCTTAACACACAGTACATTAAAAAGAACAAGCTGACAGAAGCAGACCTGCAGTATGGCTATGGTGGAGTAGATATGAATGAACCTCTGATGGAGATAGGAGAATTAGCACTGGATTTGTGGAGGAAATTAATGACAGAGCCTCTCCAAGACACGCTCCTCATAATGCTTCTCCGAGAAATTAAAAGGGATCGATGTGGGGAAGACCCAAATCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTTCCCTTAAAGTTTTATCAAGAAATATTTGAGTCGCCTTTTCTGGCTGAAACAGGGGAGTATTATAAACAAGAAGCTTCAAATTTATTACAAGAGTCAAACTGCTCACAATATATGGAAAAGATTTTAGGTAGATTAAAAGATGAAGAAATCAGATGTCGGAAGTATTTACACCCCAGCTCATATAATAAAGTGATTCACGAATGCCAGCAGAGAATGGTGGCAGATCACTTGCAGTTTCTACATGCGGAATGTCACAATATCATCAGACAAGAAAGGAGAAACGATATGGCCAACATGTATACTCTTCTTCGTGCTGTATCCAGTGGTTTACCTCATATGATTCAGGAACTCCAAAACCATATCCACGATGAAGGCCTTAGAGCTATTAGTAATCTTTCTCAGGAAAACATGCCAACTCAATTTCGGGAGTCAGTTGATGAAGTTCACAGTAAATTTGTTCAGCTTGTCAACTGTGTATTGAATGGTGATCAGCATTTTATGAGTGCACTAGACAAGGCTCTTACTTGTGTGGTGAACTACAGGGAACCAAAATCAtgaGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMSLKPRVVDFDETWNKLLTTIKAVVMLDYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVQQLHTRVLDSAEQVLVMYFRYWEEYSR
GADYMDCLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDLWRKLMTEPLQDTLLIMLLREIKRDRCGEDPNQKVIHGVINSFVHVEQYKK
KFPLKFYQEIFESPFLAETGEYYKQEASNLLQESNCSQYMEKILGRLKDEEIRCRKYLHPSSYNKVIHECQQRMVADHLQFLHAECHNIIRQERRNDMAN
MYTLLRAVSSGLPHMIQELQNHIHDEGLRAISNLSQENMPTQFRESVDEVHSKFVQLVNCVLNGDQHFMSALDKALTCVVNYREPKS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51200
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make 6His-SUMO tagged Xenopus laevis Cullin 2, residues 1-383, with the two mutations V340R and L344D. We already have a vector with a 6His-tagged version of this fragment.