cDNA Clone Cul2 (Xenopus)

• Synonyms: Cullin2
• Plasmid: pET6HIS SUMO Cul2 1-163 (xenopus)
• Parent Plasmid: pET6HIS SUMO
• Expressed: 6His SUMO Xenopus_Cul2 1-163
• Tag: SUMO
• Tag: 6His
• Species: Xenopus laevis

Sequence of Insert

CCATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCAACTGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGT
CATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATG
AACTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTCCAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGG
AACAAACGGGGGGAATGTCATTGAAGCCACGGGTAGTGGATTTTGATGAAACATGGAACAAACTTTTAACTACAATCAAAGCTGTTGTCATGCTGGATTA
TGTTGAAAGAGCAACATGGAATGACCGTTTCTCAGATATTTACGCACTGTGTGTTGCCTATCCAGAGCCTCTTGGAGAAAGATTGTATACAGAAACAAAG
ATATTTCTGGAAAACCATGTACAGCAGTTGCATACGAGAGTTCTGGATTCAGCAGAGCAAGTACTGGTAATGTACTTCAGATACTGGGAGGAATATAGCA
GAGGAGCTGATTACATGGATTGTTTATATAGGTATCTTAACACACAGTACATTAAAAAGAACAAGCTGACAGAAGCAGACCTGCAGTATGGCTATGGTGG
AGTAGATATGAATGAACCTCTGATGGAGATAGGAGAATTAGCACTGGATTTGTGGAGGAAATTAATGACAGAGCCTCTCCAAGACACGCTCCTCATAATG
CTTTAACGAGAAATTAAAAGGGATCGATGTGGGGAAGACCCAAATCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGA
AAAAATTTCCCTTAAAGTTTTATCAAGAAATATTTGAGTCGCCTTTTCTGGCTGAAACAGGGGAGTATTATAAACAAGAAGCTTCAAATTTATTACAAGA
GTCAAACTGCTCACAATATATGGAAAAGATTTTAGGTAGATTAAAAGATGAAGAAATCAGATGTCGGAAGTATTTACACCCCAGCTCATATAATAAAGTG
ATTCACGAATGCCAGCAGAGAATGGTGGCAGATCACTTGCAGTTTCTACATGCGGAATGTCACAATATCATCAGACAAGAAAGGAGAAACGATATGGCCA
ACATGTATACTCTTCTTCGTGCTGTATCCAGTGGTTTACCTCATATGATTCAGGAACTCCAAAACCATATCCACGATGAAGGCCTTAGAGCTATTAGTAA
TCTTTCTCAGGAAAACATGCCAACTCAATTTCGGGAGTCAGTTGATGAAGTTCACAGTAAATTTGTTCAGCTTGTCAACTGTGTATTGAATGGTGATCAG
CATTTTATGAGTGCACTAGACAAGGCTCTTACTTGTGTGGTGAACTACAGGGAACCAAAATCATGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMSLKPRVVDFDETWNKLLTTIKAVVMLDYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVQQLHTRVLDSAEQVLVMYFRYWEEYSR
GADYMDCLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDLWRKLMTEPLQDTLLIML*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51228
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you add 6His-SUMO (and no other amino acids) at the N-terminus of a fragment of Xenopus laevis Cul2, comprising amino acids 1-163. We already have a vector with the Xenopus Cul2 cDNA sequence. I want to make soluble protein and also generate antibodies.