cDNA Clone BMH1 (yeast)

• Plasmid: pET6HIS HALO BMH1 (yeast)
• Parent Plasmid: pET 6HIS HALO
• Expressed: His HALO BMH1
• Genbank: DU383
• Tag: HALO
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCatgtcaaccagtcgtgaagattctgtgtacctagccaagttggc
tgaacaggccgaacgttatgaagaaatggtcgaaaacatgaagactgttg
cctcctctggccaagagttgtcggtcgaagagcgtaatttgttgtctgtt
gcttataagaacgttattggtgctcgtcgtgcctcttggagaattgtttc
ttctattgagcaaaaggaggagtccaaggagaagtccgaacaccaggtcg
agttgatttgttcgtaccgttcgaagattgagaccgaactaactaagatc
tccgacgatattttgtccgtgctagactcccacttaattccatcagccac
cactggcgagtccaaggttttctactataagatgaagggtgactaccacc
gttatttggctgaattttctagtggcgatgctagagaaaaggccacaaac
gcctctttagaagcatacaagaccgcttctgaaattgccaccacagagtt
acccccaactcacccaatccgtctaggtttggctcttaacttctctgtct
tctattatgaaattcaaaactctccagacaaagcctgccatttgcgcaag
caagcttttgacgacgctattgctgagttggacactctgtctgaagaatc
atacaaagatagcacacttatcatgcaactgctaagggacaatttaacct
tatggacttcagacatgtccgagtccggtcaagctgaagaccaacaacaa
caacaacaacatcagcaacagcagccacctgctgccgccgaaggtgaagc
accaaagtaagcggccgc

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGE
RMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNP
ERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA
LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSMSTSREDSVYLAK
LAEQAERYEEMVENMKTVASSGQELSVEERNLLSVAYKNVIGARRASWRI
VSSIEQKEESKEKSEHQVELICSYRSKIETELTKISDDILSVLDSHLIPS
ATTGESKVFYYKMKGDYHRYLAEFSSGDAREKATNASLEAYKTASEIATT
ELPPTHPIRLGLALNFSVFYYEIQNSPDKACHLRKQAFDDAIAELDTLSE
ESYKDSTLIMQLLRDNLTLWTSDMSESGQAEDQQQQQQHQQQQPPAAAEG
EAPK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51316
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: For purification of HALO-BMH1 from bacteria