cDNA Clone UBA1 (yeast)

• Plasmid: pSC-B UBA1 (yeast)
• Parent Plasmid: pSC-B
• Genbank: Z28210.1
• Species: Yeast

Sequence of Insert

AGATCTATGAGCAGCAATAATAGTGGTCTCTCTGCCGCCGGAGAAATCGATGAAAGTCTTTATTCTCGTCAACTTTATGTGTTGGGTAAGGAAGCAATGT
TGAAAATGCAAACCTCCAACGTATTAATTCTCGGCTTGAAGGGGCTAGGTGTTGAGATAGCGAAGAACGTTGTGTTGGCAGGTGTCAAATCTATGACCGT
TTTTGACCCTGAGCCGGTTCAATTGGCAGACTTATCAACCCAATTCTTTTTGACCGAAAAGGATATTGGTCAAAAGAGAGGAGATGTGACCAGAGCCAAA
TTGGCAGAGTTGAACGCATATGTCCCGGTTAATGTGCTGGATTCCTTAGACGATGTTACCCAATTGAGTCAATTCCAGGTCGTTGTCGCTACGGACACTG
TTTCTTTGGAAGATAAGGTGAAAATCAACGAATTTTGCCATTCCTCCGGTATTAGATTCATTTCCTCGGAGACAAGAGGGCTGTTTGGTAACACCTTCGT
AGACCTAGGTGACGAGTTCACAGTGTTGGACCCAACGGGTGAAGAACCTCGCACTGGTATGGTTTCAGACATCGAGCCCGATGGAACAGTGACCATGCTA
GATGATAACAGACACGGGTTGGAAGACGGAAATTTTGTCAGGTTCTCCGAAGTAGAAGGTCTTGATAAGTTGAATGATGGGACCCTTTTTAAAGTTGAGG
TTTTGGGGCCCTTTGCATTCAGAATTGGTTCAGTCAAAGAATATGGCGAGTATAAGAAGGGCGGTATCTTCACCGAAGTCAAAGTACCCCGTAAAATCTC
ATTCAAGTCTTTGAAACAACAACTGTCCAATCCTGAGTTTGTTTTTTCGGATTTTGCTAAATTTGACAGAGCTGCTCAATTGCATTTGGGATTTCAGGCT
TTACATCAGTTTGCCGTTAGACATAATGGTGAACTGCCAAGAACAATGAATGACGAAGACGCCAATGAATTAATCAAGTTGGTCACCGATTTGTCCGTTC
AGCAACCGGAGGTTCTGGGTGAAGGTGTAGATGTTAATGAAGATTTAATCAAAGAGCTTTCCTATCAGGCAAGAGGTGACATACCGGGTGTCGTAGCATT
TTTCGGTGGCCTTGTGGCACAAGAAGTGTTAAAAGCATGTTCTGGTAAATTTACTCCGCTAAAGCAATTTATGTACTTTGATTCCTTGGAATCATTGCCA
GATCCTAAAAACTTCCCAAGAAATGAGAAGACTACTCAGCCAGTAAACTCTCGTTACGATAATCAAATCGCGGTTTTCGGTTTGGATTTTCAGAAAAAGA
TTGCCAATTCAAAGGTCTTTCTTGTCGGATCTGGTGCCATTGGTTGTGAGATGTTGAAAAATTGGGCACTTTTGGGGCTTGGTTCTGGCTCCGACGGTTA
CATCGTTGTTACGGATAACGATTCGATCGAAAAGTCCAACTTAAACCGTCAGTTCTTGTTTAGACCAAAGGATGTCGGAAAGAACAAATCTGAAGTAGCG
GCAGAGGCCGTTTGTGCTATGAACCCCGATTTGAAGGGCAAGATAAATGCTAAAATTGATAAAGTGGGGCCAGAAACTGAGGAAATATTTAATGACTCCT
TTTGGGAAAGCCTGGATTTTGTCACCAACGCTCTAGACAATGTCGACGCAAGAACATACGTAGATCGCCGTTGTGTGTTTTATAGAAAACCACTGTTAGA
ATCTGGTACCCTAGGTACTAAAGGTAATACTCAAGTCATCATTCCAAGATTGACTGAATCATACTCTTCTTCTAGAGACCCACCAGAAAAGTCTATCCCA
TTGTGTACCCTACGTTCTTTCCCAAACAAGATTGATCACACCATTGCCTGGGCCAAATCTCTTTTCCAAGGTTACTTCACTGATTCCGCCGAAAACGTTA
ATATGTACTTGACACAACCGAATTTCGTTGAGCAAACACTAAAGCAAAGCGGGGATGTCAAGGGAGTTCTAGAATCCATCTCAGATTCTCTATCTAGTAA
ACCACACAATTTTGAAGATTGTATCAAATGGGCAAGGTTAGAATTTGAAAAAAAATTCAACCATGATATCAAGCAGTTACTGTTCAACTTCCCCAAGGAT
GCCAAGACTTCCAACGGTGAACCATTTTGGTCCGGTGCTAAGCGTGCTCCAACACCATTAGAGTTTGATATTTACAATAACGATCACTTCCATTTTGTCG
TAGCAGGTGCTAGTTTACGCGCTTATAATTATGGTATTAAAAGTGATGACTCTAATTCTAAACCCAACGTGGACGAGTATAAGTCCGTCATCGATCATAT
GATAATTCCAGAATTTACTCCAAATGCCAATTTGAAAATTCAAGTTAATGACGATGATCCGGATCCAAATGCCAACGCGGCTAATGGCTCAGATGAAATT
GATCAGCTGGTATCATCCCTACCAGATCCTTCTACTTTGGCTGGCTTTAAACTGGAGCCTGTAGATTTCGAGAAAGACGATGACACTAATCATCATATTG
AATTTATAACGGCTTGTTCTAACTGTAGAGCTCAAAACTACTTCATCGAAACTGCTGATCGCCAAAAGACCAAATTCATAGCTGGCCGTATTATTCCTGC
CATCGCAACAACCACTTCTTTGGTTACTGGTTTGGTCAATTTGGAATTATATAAACTGATTGACAACAAGACAGATATTGAGCAATATAAGAATGGCTTT
GTTAATTTAGCTTTGCCATTTTTCGGTTTTTCGGAACCAATTGCTTCACCAAAGGGAGAATATAACAACAAGAAATATGATAAAATTTGGGATAGATTTG
ATATCAAGGGAGATATTAAATTAAGTGATTTAATTGAACATTTTGAAAAAGATGAAGGGTTAGAAATAACAATGCTTTCTTATGGCGTTTCTCTTCTTTA
TGCCTCATTTTTCCCTCCAAAGAAATTGAAAGAAAGACTAAATCTACCAATAACTCAATTGGTTAAATTAGTGACTAAGAAAGATATTCCGGCACATGTA
TCTACAATGATTCTCGAAATTTGCGCAGATGACAAGGAAGGAGAGGATGTTGAAGTTCCTTTCATTACCATTCATCTATGAGCGGCCGC

• Vector Type: Baculovirus
• Antibiotic: Amp; Kan
• DU Number: DU51208
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a vector for baculovirus expression of the budding yeast E1 for ubiquitin, which is called Uba1, with a 6His-TEV tag at the amino terminus? Then pass to Axel for expression. Thanks very much.