cDNA Clone Ctf4

• Plasmid: pGAD CTF4 (yeast) 1-927 I901E
• Parent Plasmid: pGAD
• Expressed: GalAD-Ctf4(1-927) I901E
• Genbank: NP_015461.1
• Tag: GAL4 DNA-AD
• Species: Yeast

Sequence of Insert

CATATGGCCATGGAGGCCAGTGAATTCCACCCGGGGGGAGCTGGTGCAGGCGCTGGAGCGGGGGCCATGGTTTCAGTTATAGACAAGCTTGTTTTTGACT
TTGGTGGGAAAACTCTGGTCTCTCTCGCACCAGATAATAATACTTTGTGTGTAGCCAATAAAAATGGCTTAACCAAGATTCTGAAGACAAATAACCCAGA
AGAAGAGCCAGAGACTTTAGATTCTTCCAAATTGGTCTCGTCTATAAAATGCTATTCAAACTCGCACTTTCTGATGACTACAATGCAAGGTGATGCTCTT
AGGTATAACATCGATTCTAGTCAAGAAGAATTATTGGCTAGATTTGCTTTACCCCTACGTGACTGCTGTGTTATTCATTCAGGTAAAATGGCCGTATTTG
GAGGAGACGACTTAGAACTAATCCTTTTGGAATTGGACGATGAAACACACAAAAAACATGCCATTAAAATCGATGAACAAGTTTCTCAAATTTCTTACAA
TTCACAGATGAATATTTTAGCAGTTTCAATGATAAATGGTAAGGTACAAATTTTTTCTCTGACATCTACTATTCCAAACAAAGTTCATGAGTTAAATGAT
TACATAGTGGCCAATTCTTATGATGATACACACAGAGATAAGATACTCTCGAATATGATGGATGATATAGATAAAGACAATGATAATGACCTGAGTGAAA
CGGCTGATCCAGATGAGAACAATGTAGCTGATCCAGAATTCTGTGCTGCTAATAGAATTTGCACGAGAGTGGCTTGGCATCCGAAGGGTCTGCATTTTGC
GCTACCATGTGCAGATGATACAGTAAAAATATTCTCTATAAAGGGATATTCCCTACAAAAGACGTTGTCCACAAATCTCTCATCAACAAAGGCTCATTTC
ATTGATTTGCAATTTGACCCGTTACGTGGAACTTACATTGCGGCAGTAGATTTAAATAATAAGTTAACGGTATGGAATTGGGAAACTTCCGAGATCCACT
ACACCAGGGAATTCAAAAGAAAAATTACTAATATTGCCTGGAAAATCCAAGCGGACTCAAAAACTCTAGACCTTGTTTTAGGTACCTGGTCCGGTAGTAT
AGCCATTGTCCAAAATTTGGCAGAGTCCGTAGTATCTAATATACCTGACCAATCTGTTGCTGAATCTTCAACTAAACATGGGCTTTTTGTAGACTCCGAA
TCTGACTTGGAAAACTTAGAGGGAAATGATGATATAAACAAAAGCGATAAGCTATTTTCAGATATTACTCAAGAAGCGAATGCGGAAGATGTGTTCACTC
AAACACACGACGGCCCCAGTGGATTAAGTGAAAAGAGAAAATACAACTTCGAAGATGAAGAAGACTTTATTGATGATGACGATGGTGCTGGTTATATTAG
TGGCAAAAAACCACATAATGAACATTCTTATTCGAGAGTACACAAGACTCATTCGTTTCCAATCAGTTTGGCAAACACAGGAAAATTTCGTTATATGCCT
TTTTCTCCAGCGGGAACACCTTTTGGCTTTACTGACAGGCGTTATTTGACAATGAATGAAGTGGGCTACGTATCTACTGTCAAGAATAGTGAGCAATACA
GCATAACTGTCTCTTTTTTTGATGTTGGACGTTTTAGAGAATACCATTTCGAGGACTTATTTGGCTACGATTTATGCTTCCTAAATGAAAAAGGCACTTT
ATTTGGCCAATCCAAAACTGGGCAGATACAATATAGGCCACACGATAGCATACATTCAAACTGGACCAAGATTATTCCTTTGCAAGCTGGTGAGAGAATA
ACAAGTGTGGCAGCCACCCCGGTTCGCGTTATTGTTGGTACATCATTAGGCTATTTCAGAAGTTTCAATCAATTCGGGGTTCCATTTGCTGTTGAAAAGA
CATCCCCAATTGTAGCGCTTACTGCTCAGAATTATAGGGTTTTTTCAGTACATTATTCGCAGTTTCATGGCCTTTCATACTCTTTATCTGAATTGGGTAC
TTCTAGTAAAAGGTACTATAAAAGAGAGTGTCCACTTCCAATGAGTTTACCAAACATTAATTCTGATATGAAAAAAGACGCAAATCTTGACTACTACAAT
TTTAATCCGATGGGCATCAAAAGTTTGTTCTTTTCAAGCTACGGAGATCCATGCATTTTTGGGTCCGACAACACGCTTCTATTGTTATCAAAGTGGAGAT
CACCAGAAGAAAGTAAATGGCTCCCTATTCTAGATAGCAACATGGAAATATGGAAGATGTCAGGAGGGAAGGAAACGACAGATATACATGTCTGGCCCTT
GGCTTTGGCGTATGACACATTGAATTGTATCTTAGTTAAGGGCAAGCATATATGGCCCGAGTTTCCCCTTCCGTTGCCATCCGAAATGGAGATTAGAATG
CCAGTATTTGTTAAGAGTAAATTACTAGAGGAAAACAAAGCTATATTAAATAAAAAGAACGAAATTGGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAG
ACAAAGAAATACAGATTCCTGTTTCTATGGCGGCGGAAGAAGAGTATCTGCGCAGCAAGGTTTTGTCAGAGCTGTTGACAGATACACTCGAAAATGACGG
TGAAATGTACGGCAACGAAAATGAGGTATTGGCAGCATTGAACGGTGCATATGATAAGGCTTTGTTACGTTTATTTGCGTCTGCATGCTCAGACCAAAAT
GTTGAAAAGGCTCTTTCGCTTGCTCATGAATTAAAACAAGATAGAGCACTTACTGCAGCCGTTAAGGAATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTA
AAAAAATTAATAATATAAGGGAAGCTAGATATGAACAGCAATTGAAATAACTCGAG

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAMEYPYDVPNYAHMAMEASEFHPGGAGAGAGAGAMVSVIDKLVFDFGGKTLVS
LAPDNNTLCVANKNGLTKILKTNNPEEEPETLDSSKLVSSIKCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCVIHSGKMAVFGGDDLELI
LLELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYIVANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDENN
VADPEFCAANRICTRVAWHPKGLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKLTVWNWETSEIHYTREFKRK
ITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIPDQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDITQEANAEDVFTQTHDGPSG
LSEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFD
VGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSPIVALT
AQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWL
PILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEIGADTEAEEGEEDKEIQIPV
SMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVKESERAELPSLVKKINNIRE
ARYEQQLK*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU51210
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: I would like a plasmid carrying the full length Ctf4 (1-927) mutated in L867A and I901A. I will provide the initial plasmid (amp resistance) and a DNA strider file with the exact sequence for the final clone.; many thanks; Fabrizio