cDNA Clone PTPN23

• Plasmid: pcDNA5D FRT TO GFP-PTPN23
• Parent Plasmid: pcDNA5D FRT TO GFP
• Expressed: GFP-PTPN23
• Genbank: NM_015466
• Tag: GFP
• Species: Human

Sequence of Insert

gaattcATGGAGGCCGTGCCCCGCATGCCCATGATCTGGCTGGACCTGAA
GGAGGCCGGTGACTTTCACTTCCAGCCAGCTGTGAAGAAGTTTGTCCTGA
AGAATTATGGAGAGAACCCAGAAGCCTACAATGAAGAACTGAAGAAGCTG
GAGTTGCTCAGACAGAATGCTGTCCGTGTCCCACGAGACTTTGAGGGCTG
TAGTGTCCTCCGCAAGTACCTCGGCCAGCTTCATTACCTGCAGAGTCGGG
TCCCCATGGGCTCGGGCCAGGAGGCCGCTGTCCCTGTCACCTGGACAGAG
ATCTTCTCAGGCAAGTCTGTGGCCCATGAGGACATCAAGTACGAGCAGGC
CTGTATTCTCTACAACCTTGGAGCGCTGCACTCCATGCTGGGGGCCATGG
ACAAGCGGGTGTCTGAGGAGGGCATGAAGGTCTCCTGTACCCATTTCCAG
TGCGCAGCCGGCGCCTTCGCCTACCTACGGGAGCACTTCCCTCAAGCCTA
CAGCGTCGACATGAGCCGCCAGATCCTTACGCTCAACGTCAACCTCATGC
TGGGCCAGGCTCAGGAGTGCCTCCTGGAGAAGTCGATGTTGGACAACAGG
AAGAGCTTTCTGGTGGCCCGCATCAGTGCACAGGTGGTAGATTACTACAA
GGAGGCATGCCGGGCCTTGGAGAACCCCGACACTGCCTCACTGCTGGGCC
GGATCCAGAAGGACTGGAAGAAACTTGTGCAGATGAAGATCTACTACTTC
GCAGCCGTGGCTCATCTGCACATGGGAAAGCAGGCCGAGGAGCAGCAGAA
GTTCGGGGAGCGGGTTGCATACTTCCAGAGCGCCCTGGACAAGCTCAATG
AAGCCATCAAGTTGGCCAAGGGCCAGCCTGACACTGTGCAAGACGCGCTT
CGCTTCACTATGGATGTCATTGGGGGAAAGTACAATTCTGCCAAGAAGGA
CAACGACTTCATTTACCATGAGGCTGTCCCAGCATTGGACACCCTTCAGC
CTGTAAAAGGAGCCCCCTTGGTGAAGCCCTTGCCAGTGAACCCCACAGAC
CCAGCTGTTACAGGCCCTGACATCTTTGCCAAACTGGTACCCATGGCTGC
CCACGAGGCCTCGTCACTGTACAGTGAGGAGAAGGCCAAGCTGCTCCGGG
AGATGATGGCCAAGATTGAGGACAAGAATGAGGTCCTGGACCAGTTCATG
GATTCAATGCAGTTGGATCCCGAGACGGTGGACAACCTTGATGCCTACAG
CCACATCCCACCCCAGCTCATGGAGAAGTGCGCGGCTCTCAGCGTCCGGC
CCGACACTGTCAGGAACCTTGTACAGTCCATGCAAGTGCTGTCAGGTGTG
TTCACGGATGTGGAGGCTTCCCTGAAGGACATCAGAGATCTGTTGGAGGA
GGATGAGCTGCTAGAGCAGAAGTTTCAGGAGGCGGTGGGCCAGGCAGGGG
CCATCTCCATCACCTCCAAGGCTGAGCTGGCAGAGGTGAGGCGAGAATGG
GCCAAGTACATGGAAGTCCATGAGAAGGCCTCCTTCACCAACAGTGAGCT
GCACCGTGCCATGAACCTGCACGTCGGCAACCTGCGCCTGCTCAGCGGGC
CGCTTGACCAGGTCCGGGCTGCCCTGCCCACACCGGCCCTCTCCCCAGAG
GACAAGGCCGTGCTGCAAAACCTAAAGCGCATCCTGGCTAAGGTGCAGGA
GATGCGGGACCAGCGCGTGTCCCTGGAGCAGCAGCTGCGTGAGCTTATCC
AGAAAGATGACATCACTGCCTCGCTGGTCACCACAGACCACTCAGAGATG
AAGAAGTTGTTCGAGGAGCAGCTGAAAAAGTATGACCAGCTGAAGGTGTA
CCTGGAGCAGAACCTGGCCGCCCAGGACCGTGTCCTCTGTGCACTGACAG
AGGCCAACGTGCAGTACGCAGCCGTGCGGCGGGTACTCAGCGACTTGGAC
CAAAAGTGGAACTCCACGCTGCAGACCCTGGTGGCCTCGTATGAAGCCTA
TGAGGACCTGATGAAGAAGTCGCAGGAGGGCAGGGACTTCTACGCAGATC
TGGAGAGCAAGGTGGCTGCTCTGCTGGAGCGCACGCAGTCCACCTGCCAG
GCCCGCGAGGCTGCCCGCCAGCAGCTCCTGGACAGGGAGCTGAAGAAGAA
GCCGCCGCCACGGCCCACAGCCCCAAAGCCGCTGCTGCCCCGCAGGGAGG
AGAGTGAGGCAGTGGAAGCAGGAGACCCCCCTGAGGAGCTGCGCAGCCTC
CCCCCTGACATGGTGGCTGGCCCACGACTGCCTGACACCTTCCTGGGAAG
TGCCACCCCGCTCCACTTTCCTCCCAGCCCCTTCCCCAGCTCCACAGGCC
CAGGACCCCACTATCTCTCAGGCCCCTTGCCCCCTGGTACCTACTCGGGC
CCCACCCAGCTGATACAGCCCAGGGCCCCAGGGCCCCATGCAATGCCCGT
AGCACCTGGGCCTGCCCTCTACCCAGCCCCTGCCTACACACCGGAGCTGG
GCCTTGTGCCCCGATCCTCCCCACAGCATGGCGTGGTGAGCAGTCCCTAT
GTGGGGGTAGGGCCGGCCCCACCAGTTGCAGGTCTCCCCTCGGCCCCACC
TCCTCAATTCTCAGGCCCCGAGTTGGCCATGGCGGTTCGGCCAGCCACCA
CCACAGTAGATAGCATCCAGGCGCCCATCCCCAGCCACACAGCCCCACGG
CCAAACCCCACCCCTGCTCCTCCCCCGCCCTGCTTCCCTGTGCCCCCACC
GCAGCCACTGCCCACGCCTTACACCTACCCTGCAGGGGCTAAGCAACCCA
TCCCAGCACAGCACCACTTCTCTTCTGGGATCCCCGCAGGTTTTCCAGCC
CCAAGGATTGGGCCCCAGCCCCAGCCCCATCCTCAGCCCCATCCTTCACA
AGCGTTTGGGCCTCAGCCCCCACAGCAGCCCCTTCCACTCCAGCATCCAC
ATCTCTTCCCACCCCAGGCCCCAGGACTCCTACCCCCACAATCCCCCTAC
CCCTATGCCCCTCAGCCTGGGGTCCTGGGGCAGCCGCCACCCCCCCTACA
CACCCAGCTCTACCCAGGTCCCGCTCAAGACCCTCTGCCAGCCCACTCAG
GGGCTCTGCCTTTCCCCAGCCCTGGGCCCCCTCAGCCTCCCCATCCCCCA
CTGGCATATGGTCCTGCCCCTTCTACCAGACCCATGGGCCCCCAGGCAGC
CCCTCTTACCATTCGAGGGCCCTCGTCTGCTGGCCAGTCCACCCCTAGTC
CCCACCTGGTGCCTTCACCTGCCCCATCTCCAGGGCCTGGTCCGGTACCC
CCTCGCCCCCCAGCAGCAGAACCACCCCCTTGCCTGCGCCGAGGCGCCGC
AGCTGCAGACCTGCTCTCCTCCAGCCCGGAGAGCCAGCATGGCGGCACTC
AGTCTCCTGGGGGTGGGCAGCCCCTGCTGCAGCCCACCAAGGTGGATGCA
GCTGAGGGTCGTCGGCCGCAGGCCCTGCGGCTGATTGAGCGGGACCCCTA
TGAACATCCTGAGAGGCTGCGGCAGTTGCAGCAGGAGCTGGAGGCCTTTC
GGGGTCAGCTGGGGGATGTGGGAGCTCTGGACACTGTCTGGCGAGAGCTG
CAAGATGCGCAGGAACATGATGCCCGAGGCCGTTCCATCGCCATTGCCCG
CTGCTACTCACTGAAGAACCGGCACCAGGATGTCATGCCCTATGACAGTA
ACCGTGTGGTGCTGCGCTCAGGCAAGGATGACTACATCAATGCCAGCTGC
GTGGAGGGGCTCTCCCCATACTGCCCCCCGCTAGTGGCAACCCAGGCCCC
ACTGCCTGGCACAGCTGCTGACTTCTGGCTCATGGTCCATGAGCAGAAAG
TGTCAGTCATTGTCATGCTGGTTTCTGAGGCTGAGATGGAGAAGCAAAAA
GTGGCACGCTACTTCCCCACCGAGAGGGGCCAGCCCATGGTGCACGGTGC
CCTGAGCCTGGCATTGAGCAGCGTCCGCAGCACCGAAACCCATGTGGAGC
GCGTGCTGAGCCTGCAGTTCCGAGACCAGAGCCTCAAGCGCTCTCTTGTG
CACCTGCACTTCCCCACTTGGCCTGAGTTAGGCCTGCCCGACAGCCCCAG
CAACTTGCTGCGCTTCATCCAGGAGGTGCACGCACATTACCTGCATCAGC
GGCCGCTGCACACGCCCATCATTGTGCACTGCAGCTCTGGTGTGGGCCGC
ACGGGAGCCTTTGCACTGCTCTATGCAGCTGTGCAGGAGGTGGAGGCTGG
GAACGGAATCCCTGAGCTGCCTCAGCTGGTGCGGCGCATGCGGCAGCAGA
GAAAGCACATGCTGCAGGAGAAGCTGCACCTCAGGTTCTGCTATGAGGCA
GTGGTGAGACACGTGGAGCAGGTCCTGCAGCGCCATGGTGTGCCTCCTCC
ATGCAAACCCTTGGCCAGTGCAAGCATCAGCCAGAAGAACCACCTTCCTC
AGGACTCCCAGGACCTGGTCCTCGGTGGGGATGTGCCCATCAGCTCCATC
CAGGCCACCATTGCCAAGCTCAGCATTCGGCCTCCTGGGGGGTTGGAGTC
CCCGGTTGCCAGCTTGCCAGGCCCTGCAGAGCCCCCAGGCCTCCCGCCAG
CCAGCCTCCCAGAGTCTACCCCAATCCCATCTTCCTCCCCACCCCCCCTT
TCCTCCCCACTACCTGAGGCTCCCCAGCCTAAGGAGGAGCCGCCAGTGCC
TGAAGCCCCCAGCTCGGGGCCCCCCTCCTCCTCCCTGGAATTGCTGGCCT
CCTTGACCCCAGAGGCCTTCTCCCTGGACAGCTCCCTGCGGGGCAAACAG
CGGATGAGCAAGCATAACTTTCTGCAGGCCCATAACGGGCAAGGGCTGCG
GGCCACCCGGCCCTCTGACGACCCCCTCAGCCTTCTGGATCCACTCTGGA
CACTCAACAAGACCTGActcgag

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFME
AVPRMPMIWLDLKEAGDFHFQPAVKKFVLKNYGENPEAYNEELKKLELLR
QNAVRVPRDFEGCSVLRKYLGQLHYLQSRVPMGSGQEAAVPVTWTEIFSG
KSVAHEDIKYEQACILYNLGALHSMLGAMDKRVSEEGMKVSCTHFQCAAG
AFAYLREHFPQAYSVDMSRQILTLNVNLMLGQAQECLLEKSMLDNRKSFL
VARISAQVVDYYKEACRALENPDTASLLGRIQKDWKKLVQMKIYYFAAVA
HLHMGKQAEEQQKFGERVAYFQSALDKLNEAIKLAKGQPDTVQDALRFTM
DVIGGKYNSAKKDNDFIYHEAVPALDTLQPVKGAPLVKPLPVNPTDPAVT
GPDIFAKLVPMAAHEASSLYSEEKAKLLREMMAKIEDKNEVLDQFMDSMQ
LDPETVDNLDAYSHIPPQLMEKCAALSVRPDTVRNLVQSMQVLSGVFTDV
EASLKDIRDLLEEDELLEQKFQEAVGQAGAISITSKAELAEVRREWAKYM
EVHEKASFTNSELHRAMNLHVGNLRLLSGPLDQVRAALPTPALSPEDKAV
LQNLKRILAKVQEMRDQRVSLEQQLRELIQKDDITASLVTTDHSEMKKLF
EEQLKKYDQLKVYLEQNLAAQDRVLCALTEANVQYAAVRRVLSDLDQKWN
STLQTLVASYEAYEDLMKKSQEGRDFYADLESKVAALLERTQSTCQAREA
ARQQLLDRELKKKPPPRPTAPKPLLPRREESEAVEAGDPPEELRSLPPDM
VAGPRLPDTFLGSATPLHFPPSPFPSSTGPGPHYLSGPLPPGTYSGPTQL
IQPRAPGPHAMPVAPGPALYPAPAYTPELGLVPRSSPQHGVVSSPYVGVG
PAPPVAGLPSAPPPQFSGPELAMAVRPATTTVDSIQAPIPSHTAPRPNPT
PAPPPPCFPVPPPQPLPTPYTYPAGAKQPIPAQHHFSSGIPAGFPAPRIG
PQPQPHPQPHPSQAFGPQPPQQPLPLQHPHLFPPQAPGLLPPQSPYPYAP
QPGVLGQPPPPLHTQLYPGPAQDPLPAHSGALPFPSPGPPQPPHPPLAYG
PAPSTRPMGPQAAPLTIRGPSSAGQSTPSPHLVPSPAPSPGPGPVPPRPP
AAEPPPCLRRGAAAADLLSSSPESQHGGTQSPGGGQPLLQPTKVDAAEGR
RPQALRLIERDPYEHPERLRQLQQELEAFRGQLGDVGALDTVWRELQDAQ
EHDARGRSIAIARCYSLKNRHQDVMPYDSNRVVLRSGKDDYINASCVEGL
SPYCPPLVATQAPLPGTAADFWLMVHEQKVSVIVMLVSEAEMEKQKVARY
FPTERGQPMVHGALSLALSSVRSTETHVERVLSLQFRDQSLKRSLVHLHF
PTWPELGLPDSPSNLLRFIQEVHAHYLHQRPLHTPIIVHCSSGVGRTGAF
ALLYAAVQEVEAGNGIPELPQLVRRMRQQRKHMLQEKLHLRFCYEAVVRH
VEQVLQRHGVPPPCKPLASASISQKNHLPQDSQDLVLGGDVPISSIQATI
AKLSIRPPGGLESPVASLPGPAEPPGLPPASLPESTPIPSSSPPPLSSPL
PEAPQPKEEPPVPEAPSSGPPSSSLELLASLTPEAFSLDSSLRGKQRMSK
HNFLQAHNGQGLRATRPSDDPLSLLDPLWTLNKT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24946
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41455. Compared to NCB! ref clone has silent changes at Thr329 (ACT>ACC), Pro933 (CCG>CCA), Glu1166 (GAG>GAA), Pr01545 (CCG>CCA)