cDNA Clone Codanin 1 (CDAN1)

• Synonyms: CDA1; CDAI; CDAN1A; DLT; PRO1295
• Plasmid: pcDNA5D FRT/TO FLAG CDAN1 (Killed internal NotI)
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG CDAN1
• Genbank: NM_138477.2 GI:57222569 with residue difference S421F (present in many published sequences for CDAN1)
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCGTTTTGGAGTCGCTGCTGCGAGAAGAGGTGTCGGTCGCAGCCGTCGTGCGGTGGATCGCGCGCAGCACCCAGGGTTCGGAGGATAACGCTGGGGAGGCGGCTGCGCTGAGCTCACTCCGGGCCCTGCGGAAAGAATTCGTACCGTTCCTGTTGAACTTCCTGAGGGAGCAGAGCAGCCGCGTCCTCCCGCAGGGGCCCCCGACCCCCGCCAAGACCCCGGGCGCCTCGGCAGCCTTGCCAGGGAGGCCGGGAGGCCCGCCGCGGGGTAGCCGCGGGGCGCGCAGCCAGCTTTTCCCTCCGACCGAGGCCCAGAGCACCGCTGCCGAGGCCCCTCTGGCCCGCCGCGGGGGCAGGAGGCGGGGCCCGGGGCCGGCCCGCGAGCGTGGAGGCCGCGGCCTGGAGGAGGGGGTCAGCGGGGAGAGCCTGCCCGGAGCCGGGGGCCGGAGGCTTAGGGGCTCTGGCAGCCCCAGCCGCCCCAGCCTCACGCTGTCTGATCCGCCAAACCTCAGCAACCTGGAGGAGTTCCCTCCCGTAGGCTCGGTTCCCCCCGGCCCTACAGGGACGAAGCCTTCTCGCAGGATCAACCCAACTCCGGTGAGCGAAGAGCGGTCACTCTCCAAGCCCAAGACCTGCTTCACCTCACCCCCAATCAGCTGTGTCCCCAGTTCCCAACCCTCAGCCCTGGACACTAGCCCTTGGGGCCTTGGCCTTCCCCCAGGGTGCAGAAGTCTGCAAGAGGAGCGAGAGATGCTCAGGAAGGAGCGCTCTAAGCAGCTGCAGCAGTCACCTACCCCCACCTGTCCCACCCCAGAATTGGGGTCGCCCCTCCCCAGCCGGACAGGAAGCCTCACAGATGAACCTGCAGACCCTGCCAGAGTGTCTTCCCGCCAGCGCCTGGAGCTTGTAGCCCTTGTTTACTCCTCGTGCATTGCTGAGAACCTGGTACCAAACCTCTTCTTGGAGCTTTTCTTCGTCTTTCAGCTCCTCACTGCCCGGAGGATGGTGACTGCCAAGGACAGCGACCCTGAACTAAGTCCAGCTGTCCTAGATTCCCTGGAAAGTCCACTGTTCCAAAGCATCCACGATTGTGTCTTCTTTGCAGTGCAGGTTTTGGAGTGTCACTTTCAGGTTCTTTCCAACCTGGACAAAGGGACCTTGAAGCTGCTGGCTGAGAATGAGCGGCTGCTGTGCTTCTCACCAGCTCTGCAAGGCCGCCTTCGAGCTGCCTATGAGGGCAGTGTTGCCAAGGTCTTTCTGGTGATGCCTCCCTCTACTCAAGCTGTCTCCTTTCAGCCAGAGACTGACAATCGTGCCAACTTCTCCAGTGACCGAGCCTTTCATACTTTTAAGAAACAGAGGGATGTGTTTTATGAGGTGCTGCGAGAGTGGGAAGATCACCATGAGGAGCCTGGCTGGGATTTTGAGAAGGGCTTGGGCAGCAGAATCAGGGCCATGATGGGTCAGCTCTCCGCAGCCTGCAGCCACAGCCACTTTGTTCGGCTTTTCCAAAAACAACTACTCCAGATGTGTCAGAGCCCTGGTGGTGCTGGGGGCACCGTCTTGGGCGAGGCCCCAGATGTGTTGAGTATGCTGGGAGCTGACAAGCTGGGGCGGTTGTGGCGCCTACAGGAACGGCTTATGGCTCCTCAGAGCAGTGGGGGGCCCTGCCCACCCCCCACCTTCCCAGGCTGTCAAGGCTTCTTTAGGGACTTCATCCTTAGTGCCAGCAGCTTCCAGTTTAACCAGCATCTCATGGACAGTCTGAGCTTGAAGATCCAGGAGCTCAATGGTCTTGCCCTGCCCCAGCATGAGCCCAATGATGAAGACGGGGAGTCAGACGTAGACTGGCAGGGTGAGCGGAAGCAATTTGCTGTGGTGCTTCTTAGCCTGAGACTTTTGGCTAAATTCCTGGGCTTTGTGGCTTTCCTGCCATACCGGGGGCCTGAACCTCCCCCGACCGGTGAGCTTCAGGACTCCATTCTGGCCCTCAGGAGCCAGGTCCCTCCGGTCCTGGATGTGCGGACTCTGCTGCAGCGAGGGCTGCAGGCCCGCCGGGCGGTGCTCACCGTGCCCTGGCTGGTGGAGTTTCTCTCCTTTGCTGACCATGTTGTTCCCTTGCTGGAATATTACCGGGACATCTTCACTCTCCTGCTGCGCCTGCACCGGAGCTTGGTGTTGTCGCAGGAGAGTGAGGGGAAGATGTGTTTCCTGAACAAGCTGCTGCTACTTGCTGTCCTGGGCTGGCTTTTCCAGATTCCCACAGTCCCTGAGGACTTGTTCTTTCTGGAAGAGGGTCCCTCATATGCCTTTGAGGTGGACACAGTAGCCCCAGAGCATGGCTTGGACAATGCGCCTGTGGTGGACCAGCAGCTGCTCTACACCTGCTGCCCCTACATCGGAGAGCTCCGGAAACTGCTCGCTTCGTGGGTGTCAGGCAGTAGTGGACGGAGTGGGGGCTTCATGAGGAAAATCACCCCCACCACTACCACCAGCCTGGGAGCCCAGCCTTCCCAGACCAGCCAGGGGCTGCAGGCACAGCTCGCCCAGGCCTTTTTCCACAACCAGCCGCCCTCCTTGCGCCGGACCGTAGAGTTCGTGGCAGAAAGAATTGGATCAAACTGTGTCAAACATATCAAGGCTACACTGGTGGCAGATCTGGTGCGCCAGGCAGAGTCACTTCTCCAAGAGCAGCTGGTGACACAGGGAGAGGAAGGGGGAGACCCAGCCCAGCTGTTGGAGATCTTGTGTTCCCAGCTGTGCCCTCACGGGGCCCAGGCATTGGCCCTGGGGCGGGAGTTCTGTCAAAGGAAGAGCCCTGGGGCTGTGCGGGCGCTGCTTCCAGAGGAGACCCCGGCAGCCGTTCTGAGCAGTGCAGAGAACATTGCTGTGGGGCTTGCAACAGAGAAAGCCTGTGCTTGGCTGTCAGCCAACATCACAGCACTGATCAGGAGGGAGGTGAAAGCAGCAGTGAGTCGCACACTTCGAGCCCAGGGTCCTGAACCTGCTGCCCGGGGGGAGCGGAGGGGCTGCTCCCGCGCCTGTGAGCACCATGCTCCCCTCCCCTCCCACCTCATCTCCGAGATAAAAGACGTGCTCTCCTTGGCCGTGGGGCCACGGGACCCTGACGAGGGAGTCTCCCCAGAGCATCTGGAACAGCTCCTAGGCCAGCTGGGCCAGACGCTGCGGTGCCGCCAGTTCCTGTGCCCACCTGCTGAGCAGCATCTGGCAAAGTGCTCTGTGGAGTTAGCTTCCCTCCTCGTTGCAGATCAAATTCCTATCCTAGGGCCCCCGGCACAGTACAGGCTGGAGAGAGGGCAGGCTCGAAGGCTTCTGCACATGCTGCTTTCCTTGTGGAAGGAAGACTTTCAGGGGCCGGTTCCGCTGCAGCTGCTGCTGAGCCCAAGAAATGTGGGGCTTCTGGCAGACACAAGGCCAAGGGAGTGGGACTTGCTGCTATTCTTGCTACGGGAGCTGGTGGAGAAGGGTCTGATGGGACGGATGGAGATAGAGGCCTGCCTGGGCAGCCTCCACCAGGCCCAGTGGCCAGGGGACTTTGCTGAAGAATTAGCAACACTGTCTAATCTGTTTCTAGCCGAGCCCCACCTGCCAGAACCCCAGCTAAGAGCCTGTGAGTTGGTGCAGCCAAACCGGGGCACTGTGCTGGCCCAGAGCTAGCTCGAGAAGCTTGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMAAVLESLLREEVSVAAVVRWIARSTQGSEDNAGEAAAL
SSLRALRKEFVPFLLNFLREQSSRVLPQGPPTPAKTPGASAALPGRPGGP
PRGSRGARSQLFPPTEAQSTAAEAPLARRGGRRRGPGPARERGGRGLEEG
VSGESLPGAGGRRLRGSGSPSRPSLTLSDPPNLSNLEEFPPVGSVPPGPT
GTKPSRRINPTPVSEERSLSKPKTCFTSPPISCVPSSQPSALDTSPWGLG
LPPGCRSLQEEREMLRKERSKQLQQSPTPTCPTPELGSPLPSRTGSLTDE
PADPARVSSRQRLELVALVYSSCIAENLVPNLFLELFFVFQLLTARRMVT
AKDSDPELSPAVLDSLESPLFQSIHDCVFFAVQVLECHFQVLSNLDKGTL
KLLAENERLLCFSPALQGRLRAAYEGSVAKVFLVMPPSTQAVSFQPETDN
RANFSSDRAFHTFKKQRDVFYEVLREWEDHHEEPGWDFEKGLGSRIRAMM
GQLSAACSHSHFVRLFQKQLLQMCQSPGGAGGTVLGEAPDVLSMLGADKL
GRLWRLQERLMAPQSSGGPCPPPTFPGCQGFFRDFILSASSFQFNQHLMD
SLSLKIQELNGLALPQHEPNDEDGESDVDWQGERKQFAVVLLSLRLLAKF
LGFVAFLPYRGPEPPPTGELQDSILALRSQVPPVLDVRTLLQRGLQARRA
VLTVPWLVEFLSFADHVVPLLEYYRDIFTLLLRLHRSLVLSQESEGKMCF
LNKLLLLAVLGWLFQIPTVPEDLFFLEEGPSYAFEVDTVAPEHGLDNAPV
VDQQLLYTCCPYIGELRKLLASWVSGSSGRSGGFMRKITPTTTTSLGAQP
SQTSQGLQAQLAQAFFHNQPPSLRRTVEFVAERIGSNCVKHIKATLVADL
VRQAESLLQEQLVTQGEEGGDPAQLLEILCSQLCPHGAQALALGREFCQR
KSPGAVRALLPEETPAAVLSSAENIAVGLATEKACAWLSANITALIRREV
KAAVSRTLRAQGPEPAARGERRGCSRACEHHAPLPSHLISEIKDVLSLAV
GPRDPDEGVSPEHLEQLLGQLGQTLRCRQFLCPPAEQHLAKCSVELASLL
VADQIPILGPPAQYRLERGQARRLLHMLLSLWKEDFQGPVPLQLLLSPRN
VGLLADTRPREWDLLLFLLRELVEKGLMGRMEIEACLGSLHQAQWPGDFA
EELATLSNLFLAEPHLPEPQLRACELVQPNRGTVLAQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50592
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mutagenesis of DU49698 to kill NotI in CDAN1. Allows excision of CDAN1 insert BamHI/NotI.