cDNA Clone FAM83G

• Synonyms: PAWS1
• Plasmid: pSCB FAM83G 3FNeon donor req repair
• Parent Plasmid: pSC-B
• Genbank: NM_001039999.2
• Species: Human

Sequence of Insert

GCGGCCGCCACTCTAAGAAGCTACTGGGAAGTTCCCCGGCTGCCCTAGTTTCCCTCAGAAGTTGCGAGGACTTCAGGCAGCTCCGGAAATCTGGGATATGGGTTCCCTGCACGTAGAGGCACGCTGAGCCCTGGGACGTGGCCCACCGCTCCCTCACCGCTGTTCCGATCCCTTACTTTCTTCCCATCCCAGTCCAGTGCCCCCACCTCATCTTCCCAGCCCCAGCCCCAGCCCCGTCCTTCCCACGCCCACCTGAGTTCCCGGCCCCGCCCCCGCCGCCCCTCCCCGCGCGGCCCGCCCTCCCCGCCGAGCCGGGCTCATCAGGTCTTTCCCGCAGATTGCTCATGGGCAGAGAAGCAGAGGCCGCGGAGAGGCCCCCGAGCGCGCGGTGCCCCAGCTGGGCCGGAGCTAGGAGCTGGACCTTGCCCACCCACCCCCGGCAGCAGTGCCCGGGCAGGCGGCGCCATGGACTACAAGGACCACGACGATAAGGGATCTATGGTGAGCAAGGGCGAGGAGGATAACATGGCCTCTCTCCCAGCGACACATGAGTTACACATCTTTGGCTCCATCAACGGTGTGGACTTTGACATGGTGGGTCAGGGCACCGGCAATCCAAATGATGGTTATGAGGAGTTAAACCTGAAGTCCACCAAGGGTGACCTCCAGTTCTCCCCCTGGATTCTGGTCCCTCATATCGGGTATGGCTTCCATCAGTACCTGCCCTACCCTGACGGGATGTCGCCTTTCCAGGCCGCCATGGTAGATGGCTCCGGATACCAAGTCCATCGCACAATGCAGTTTGAAGATGGTGCCTCCCTTACTGTTAACTACCGCTACACCTACGAGGGAAGCCACATCAAAGGAGAGGCCCAGGTGAAGGGGACTGGTTTCCCTGCTGACGGTCCTGTGATGACCAACTCGCTGACCGCTGCGGACTGGTGCAGGTCGAAGAAGACTTACCCCAACGACAAAACCATCATCAGTACCTTTAAGTGGAGTTACACCACTGGAAATGGCAAGCGCTACCGGAGCACTGCGCGGACCACCTACACCTTTGCCAAGCCAATGGCGGCTAACTATCTGAAGAACCAGCCGATGTACGTGTTCCGTAAGACGGAGCTCAAGCACTCCAAGACCGAGCTCAACTTCAAGGAGTGGCAAAAGGCCTTTACCGATGTGATGGGCATGGACGAGCTGTACAAGGCTTTCAGCCAAGTGCAGTGTCTGGACGATAAACACGTCAATTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCGAGGAGCAGCGGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAGGTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAAGCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTCGGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGCGAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCCCTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGGACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGCGTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGTGCGGAAGATGATCAGCCAGGCACAGAAGGTGAGGCAGCGCGGGGACTCCTGGAGCCATGGCCCAACCAGCCACCGGACCCCTCTCAATACAGGCTCACTCAGAGCCCAAAACGGGAGCCCAACATGGGCTTCTAGGGATCTCTGCTGTTTTCCCTGGCTGCCTCTTGGGTGGAGGTTTCCTCCCCACCTTCGTGGGGATCCGCAGTTTCTTTGCAGGGTAGGGTTCCCAGGTCCTAGCAGGTGACAGTGGGCTGCCCCGTCTCAAAGGGCCTCTTTATTGTTGGAGGGTGTTTCTGGTTCCCCAGAAGAAGCGGCCGC

Amino Acid Sequence

MDYKDHDDKGSMVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMV
DGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAK
PMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYK.....FAM83G

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU52375
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: FAM83G donor designed to target the ATG start codon to insert an in-frame triple FLAG tag and mNeon reporter. Missing some FLAG tags and requires repair.