cDNA Clone Gen1

• Synonyms: Gen
• Plasmid: pET15 6His MBP TEV Gen1
• Parent Plasmid: pET15 6HIS MBP DU
• Expressed: HIS-MBP-Gen1
• Genbank: NM_001130009.1 GI:194018534*
• Tag: 6His
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGGGAGTGAATGACTTGTGGCAAATTTTGGAGCCTGTTAAGCAACACATCCCCTTGCGTAATCTTGGTGGGAAAACCATTGCAGTTGATCTGAGTCTCTGGGTGTGTGAGGCACAGACAGTCAAAAAAATGATGGGCAGCGTCATGAAGCCCCACCTCAGGAACTTATTTTTTCGTATCTCATATTTAACACAAATGGATGTAAAACTGGTATTTGTTATGGAAGGGGAACCACCAAAGCTGAAAGCTGATGTCATAAGCAAGAGGAATCAGACTCGGTATGGGTCTTCTGGAAAATCGTGGTCTCAGAAAACAGGGAGATCACATTTTAAATCAGTCTTAAGAGAGTGCCTCCATATGCTCGAATGCTTAGGAATCCCCTGGGTTCAGGCTGCTGGGGAAGCTGAAGCCATGTGTGCTTATCTCAATGCTGGTGGTCATGTCGATGGCTGCCTCACCAATGATGGAGATACTTTCCTTTATGGGGCCCAGACTGTTTACAGGAATTTCACTATGAATACAAAGGACCCACATGTTGACTGTTACACAATGTCATCTATCAAGAGTAAACTAGGTTTGGATAGAGATGCTCTGGTTGGATTAGCAATACTTCTTGGCTGTGATTATCTCCCAAAGGGAGTCCCTGGAGTTGGAAAAGAGCAAGCATTAAAACTTATACAGATTTTGAAAGGGCAAAGTTTACTTCAGAGGTTTAATCGGTGGAATGAAACATCTTGTAACTCTAGTCCACAACTGCTAGTCACTAAAAAACTGGCTCATTGTTCCGTATGTTCCCATCCAGGTTCACCTAAGGATCATGAACGTAATGGATGCAGATTATGTAAAAGTGATAAATATTGTGAGCCACATGACTATGAATACTGCTGTCCTTGTGAGTGGCACCGTACAGAACATGATAGGCAACTCAATGAAGTAGAGAACAATATTAAGAAGAAAGCTTGCTGTTGTGAGGGATTCCCATTCCATGAGGTTATTCAAGAATTCCTTTTAAACAAGGATAAATTGGTGAAGGTTATCAGGTACCAAAGACCTGATTTGTTATTGTTTCAGAGATTTACTCTTGAAAAAATGGAGTGGCCCAATCACTATGCATGTGAGAAATTGCTGGTACTTTTGACCCATTATGACATGATAGAAAGAAAGCTTGGTAGCAGAAACTCTAATCAACTACAGCCAATTCGAATTGTTAAGACTCGAATCAGAAATGGAGTTCATTGTTTTGAAATAGAATGGGAAAAGCCTGAACATTATGCTATGGAAGATAAACAACATGGAGAATTTGCTTTATTAACAATTGAGGAAGAATCATTGTTTGAAGCAGCATATCCTGAGATCGTTGCTGTTTACCAAAAACAAAAGTTAGAAATTAAAGGGAAGAAACAAAAACGTATTAAGCCTAAAGAAAACAATTTGCCAGAACCAGATGAAGTAATGAGCTTTCAGTCACACATGACTTTAAAACCCACATGTGAAATCTTTCATAAGCAGAATTCCAAGTTAAATTCGGGGATTTCCCCTGATCCTACATTACCACAGGAATCTATTTCTGCCTCATTGAATAGCTTGCTTTTACCTAAAAATACTCCATGTTTGAATGCACAAGAACAGTTCATGTCTTCTCTAAGACCTTTGGCTATACAGCAAATTAAAGCTGTCAGTAAGTCTCTAATTTCAGAATCTAGTCAACCCAATACCTCATCTCATAATATATCCGTGATTGCTGATCTACACTTGAGCACTATTGACTGGGAAGGTACTTCTTTTAGTAATTCTCCAGCTATTCAAAGGAATACTTTTTCTCATGATTTAAAATCAGAAGTTGAATCAGAGCTATCAGCCATCCCTGATGGCTTTGAAAATATCCCAGAACAACTGTCCTGTGAATCAGAAAGGTACACTGCAAACATAAAGAAAGTGTTGGATGAGGATTCTGATGGGATTAGTCCTGAGGAGCATCTACTTTCTGGCATTACTGATTTATGTCTTCAGGATTTGCCTTTAAAGGAACGAATATTTATAAAATTATCATATCCTCAGGATAATCTACAACCAGATGTCAACCTGAAAACTTTGTCCATACTTAGTGTAAAAGAATCTTGTATTGCTAACAGTGGTTCTGATTGTACATCACATCTTTCAAAGGATCTTCCAGGAATTCCCTTGCAAAATGAATCCAGAGACTCTAAAATTCTAAAAGGAGACCAGCTGCTTCAAGAAGACTATAAAGTCAATACTTCTGTCCCTTATTCTGTCAGTAACACAGTGGTAAAGACCTGCAATGTTAGACCACCAAATACTGCTTTAGATCATAGTAGAAAAGTTGATATGCAAACCACTCGGAAAATTTTAATGAAGAAGAGTGTTTGCCTTGACAGACATTCCTCTGATGAACAAAGTGCCCCAGTGTTTGGGAAAGCTAAGTACACAACTCAAAGAATGAAGCACAGTTCTCAAAAGCATAATTCATCCCATTTCAAAGAAAGTGGCCATAACAAGTTGAGTAGCCCTAAGATACATATTAAAGAAACTGAACAGTGTGTCAGATCTTATGAAACAGCTGAAAATGAAGAAAGCTGTTTCCCAGATTCAACAAAAAGTTCTCTGAGTTCTCTACAATGTCATAAGAAAGAAAACAACTCTGGTACTTGTTTGGATAGCCCTCTTCCTTTACGCCAGAGATTAAAACTAAGATTCCAAAGCACTTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHGSSMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQ
DKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNA
GAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLT
DEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGENLY
FQGHMGSMGVNDLWQILEPVKQHIPLRNLGGKTIAVDLSLWVCEAQTVKKMMGSVMKPHLRNLFFRISYLTQMDVKLVFVMEGEPPKLKADVISKRNQTR
YGSSGKSWSQKTGRSHFKSVLRECLHMLECLGIPWVQAAGEAEAMCAYLNAGGHVDGCLTNDGDTFLYGAQTVYRNFTMNTKDPHVDCYTMSSIKSKLGL
DRDALVGLAILLGCDYLPKGVPGVGKEQALKLIQILKGQSLLQRFNRWNETSCNSSPQLLVTKKLAHCSVCSHPGSPKDHERNGCRLCKSDKYCEPHDYE
YCCPCEWHRTEHDRQLNEVENNIKKKACCCEGFPFHEVIQEFLLNKDKLVKVIRYQRPDLLLFQRFTLEKMEWPNHYACEKLLVLLTHYDMIERKLGSRN
SNQLQPIRIVKTRIRNGVHCFEIEWEKPEHYAMEDKQHGEFALLTIEEESLFEAAYPEIVAVYQKQKLEIKGKKQKRIKPKENNLPEPDEVMSFQSHMTL
KPTCEIFHKQNSKLNSGISPDPTLPQESISASLNSLLLPKNTPCLNAQEQFMSSLRPLAIQQIKAVSKSLISESSQPNTSSHNISVIADLHLSTIDWEGT
SFSNSPAIQRNTFSHDLKSEVESELSAIPDGFENIPEQLSCESERYTANIKKVLDEDSDGISPEEHLLSGITDLCLQDLPLKERIFIKLSYPQDNLQPDV
NLKTLSILSVKESCIANSGSDCTSHLSKDLPGIPLQNESRDSKILKGDQLLQEDYKVNTSVPYSVSNTVVKTCNVRPPNTALDHSRKVDMQTTRKILMKK
SVCLDRHSSDEQSAPVFGKAKYTTQRMKHSSQKHNSSHFKESGHNKLSSPKIHIKETEQCVRSYETAENEESCFPDSTKSSLSSLQCHKKENNSGTCLDS
PLPLRQRLKLRFQST*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50828
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * with one silent change and residue changes S92T, S310N and T680I - common variants