cDNA Clone FAM83G

• Plasmid: pCMV5D FAM83G-C-GFP
• Parent Plasmid: pCMV5 C-GFP DU
• Expressed: FAM83G-GFP
• Genbank: NM_001039999.2
• Tag: GFP

Sequence of Insert

ggatccATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAACCATGTGAACTGGCG
CTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCGAGGAGCAGCGGCTGG
CGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAGGTGCTC
AAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAAGCGCAT
CCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTCGGGGCA
CGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGCGAGGAA
GCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCCCTCCCT
GGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGGACCTGG
GCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGCGTCTAC
ATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGTGCGGAA
GATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACATGTTCA
CCGACGTGGACATCTTCAAGGACCTGCTGGACGCCGGCTTCAAGAGGAAA
GTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTTCCTGCA
CATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCAAGAATCTCAGAG
TGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACCAAGTTC
AAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGATGGAGACCGGGCTGT
GTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACCGGAATG
TGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTTGACCGGCAGTTC
CAGGAGCTGTACCTCATGTCACACAGTGTGAGCCTCAAGGGCATCCCTAT
GGAGAAGGAACCGGAGCCGGAGCCTATTGTGCTGCCCTCTGTGGTCCCCC
TGGTGCCCGCGGGCACTGTGGCCAAGAAGCTCGTCAACCCCAAGTACGCA
CTTGTCAAGGCCAAGAGCGTCGACGAGATTGCCAAGATCTCCTCTGAGAA
GCAGGAGGCCAAGAAGCCCCTGGGGCTGAAAGGCCCAGCGCTGGCTGAGC
ATCCAGGGGAACTCCCCGAGCTGCTGCCACCCATCCACCCAGGACTGCTT
CACCTGGAGAGGGCCAACATGTTTGAGTACCTGCCCACGTGGGTGGAGCC
AGACCCGGAGCCTGGCAGCGACATCCTGGGCTACATCAATATCATCGACC
CCAACATCTGGAACCCCCAGCCCAGCCAGATGAACCGCATCAAGATCCGT
GACACCTCCCAGGCCAGCGCCCAGCACCAGCTGTGGAAGCAGAGCCAGGA
CAGCAGGCCCCGTCCAGAGCCTTGCCCTCCCCCAGAGCCCAGTGCCCCCC
AGGACGGTGTCCCAGCTGAGAACGGCCTCCCCCAGGGGGACCCTGAGCCA
TTGCCCCCCGTGCCCAAGCCCCGGACAGTCCCTGTGGCAGATGTACTAGC
CCGGGACAGCAGTGATATTGGCTGGGTCCTGGAGCTCCCCAAAGAGGAAG
CTCCCCAGAATGGGACAGACCATAGGCTACCCAGGATGGCAGGCCCAGGC
CACGCCCCACTCCAGCGGCAGCTATCTGTGACCCAGGATGACCCCGAGAG
CCTCGGGGTGGGGCTCCCCAATGGGCTGGATGGGGTGGAAGAAGAAGATG
ATGACGACTACGTAACCCTCAGTGACCAGGACAGCCACTCAGGCAGCTCC
GGCCGTGGCCCTGGCCCCCGACGGCCCTCAGTGGCTTCCTCTGTGTCAGA
GGAGTACTTCGAGGTGAGAGAGCACTCAGTCCCTCTCCGGAGGCGCCACT
CAGAGCAAGTGGCCAACGGGCCAACCCCACCACCGCGCCGGCAGCTGAGT
GCCCCCCATATAACCCGAGGGACCTTTGTTGGACCCCAGGGTGGCTCCCC
ATGGGCCCAGAGTCGGGGAAGAGAAGAAGCAGATGCGTTGAAGAGGATGC
AGGCCCAGCGCTCCACAGACAAGGAGGCACAGGGCCAGCAGTTTCATCAT
CACAGGGTCCCTGCCTCAGGGACTAGGGATAAAGACGGCTTCCCAGGACC
CCCTAGGTACCGCTCTGCTGCTGACAGCGTCCAGAGCTCTACCAGAAACG
CTGGCCCAGCCATGGCTGGCCCCCACCACTGGCAGGCCAAGGGAGGTCAG
GTACCCCGCCTGCTTCCGGAcCCCGGCAGCCCAAGACTGGCCCAAAATGC
CCGCCCCATGACCGATGGCAGGGCCACCGAGGAGCATCCGAGTCCCTTCG
GAATCCCATACTCCAAACTGTCTCAGTCGAAGCACCTAAAGGCCAGGACG
GGCGGTAGCCAGTGGGCCTCATCGGATTCTAAACGGAGGGCTCAAGCCCC
CCGGGACCGCAAAGACCCCgcggccgc

Amino Acid Sequence

MAFSQVQCLDDNHVNWRSSESKPEFFYSEEQRLALEALVARGRDAFYEVL
KRENIRDFLSELELKRILETIEVYDPGSEDPRGTGPSQGPEDNGVGDGEE
ASGADGVPIEAEPLPSLEYWPQKSDRSIPQLDLGWPDTIAYRGVTRASVY
MQPPIDGQAHIKEVVRKMISQAQKVIAVVMDMFTDVDIFKDLLDAGFKRK
VAVYIIVDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFTRSATKF
KGALAQKFMFVDGDRAVCGSYSFTWSAARTDRNVISVLSGQVVEMFDRQF
QELYLMSHSVSLKGIPMEKEPEPEPIVLPSVVPLVPAGTVAKKLVNPKYA
LVKAKSVDEIAKISSEKQEAKKPLGLKGPALAEHPGELPELLPPIHPGLL
HLERANMFEYLPTWVEPDPEPGSDILGYINIIDPNIWNPQPSQMNRIKIR
DTSQASAQHQLWKQSQDSRPRPEPCPPPEPSAPQDGVPAENGLPQGDPEP
LPPVPKPRTVPVADVLARDSSDIGWVLELPKEEAPQNGTDHRLPRMAGPG
HAPLQRQLSVTQDDPESLGVGLPNGLDGVEEEDDDDYVTLSDQDSHSGSS
GRGPGPRRPSVASSVSEEYFEVREHSVPLRRRHSEQVANGPTPPPRRQLS
APHITRGTFVGPQGGSPWAQSRGREEADALKRMQAQRSTDKEAQGQQFHH
HRVPASGTRDKDGFPGPPRYRSAADSVQSSTRNAGPAMAGPHHWQAKGGQ
VPRLLPDPGSPRLAQNARPMTDGRATEEHPSPFGIPYSKLSQSKHLKART
GGSQWASSDSKRRAQAPRDRKDPAAAMVSKGEELFTGVVPILVELDGDVN
GHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSR
YPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRI
ELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
VTAAGITLGMDELYKSGL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28092
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 44023. Silent change at D757 (GAT > GAC) compared to NCBI