cDNA Clone mouse MELK

• Synonyms: AI327312; mKIAA0175; MPK38
• Plasmid: pGEX6P1 mouse MELK
• Parent Plasmid: pGEX6P1
• Expressed: GST-3C-mouse MELK
• Genbank: NM_010790.2 GI:31981625*
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGAAAGATTATGACGAACTCCTCAAATACTATGAACTATATGAAACGATTGGGACAGGTGGCTTTGCAAAGGTCAAACTGGCCTGCCATGTCCTCACTGGAGAGATGGTAGCTATAAAAATCATGGATAAGAATGCGCTAGGGAGTGATTTGCCCCGAGTCAAAACTGAGATCGATGCGCTGAAGAGTCTGAGACATCAGCACATATGTCAGCTCTACCATGTGCTGGAGACAAAGAACAAAATATTCATGGTTCTGGAGTACTGTCCAGGAGGAGAGCTGTTTGACTACATAATCTCCCAGGATCGCCTGTCGGAAGAGGAGACCCGGGTCGTCTTCCGTCAGATACTGTCTGCAGTTGCGTATGTCCACAGCCAGGGCTATGCCCACAGGGACCTCAAACCAGAAAATTTATTATTTGATGAAAATCATAAGCTAAAGCTGATTGACTTTGGTCTTTGTGCAAAACCCAAGGGCAACAAGGACTACCATCTGCAGACGTGCTGTGGGAGCCTTGCTTATGCAGCTCCTGAACTAATACAAGGGAAGTCGTACCTTGGATCAGAGGCAGATGTTTGGAGCATGGGCATCCTCCTGTATGTGCTCATGTGTGGATTTCTACCATTTGATGATGATAATGTCATGGCTTTGTACAAGAAGATAATGAGAGGGAAATACGAAGTTCCTAAGTGGCTCTCTCCCAGTAGCATTCTGCTTCTCCAGCAGATGTTGCAGGTGGACCCAAAGAAACGGATTTCTATGAGAAATCTCCTGAACCATCCCTGGGTCATGCAAGATTACAGCTGTCCCGTGGAGTGGCAAAGCAAGACTCCTTTGACTCACCTCGATGAGGATTGCGTGACAGAGCTTTCTGTACATCACCGCAGCAGCAGGCAGACAATGGAGGATTTAATTTCGTCGTGGCAGTACGATCACCTCACAGCCACCTACCTTCTGCTTCTAGCCAAGAAGGCCCGCGGGAAGCCGGCTCGTCTACAGCTCCCGTCCTTCTCTTGTGGAACCGCCAGCACCACTCCAAAGTCAAAGAATCTGAGCCTGGAAGATATGAGCACAAGTGATGATAACTGTGTGGCTGGATTGATAGACTATGAATTGTGTGAAGATAAATTATTAGCTCCCAAGACGCCACAGGTTACCAAACACTTGGCAGAATCAAATCACGCAGCATCTAAATCACCAGCGCCAGGGGTACGCAGAGCAGTGGCAAATAAATTAATGGACAAAGAAAATGTGTGCACTCCCAAGTCTTCTGTGAAGAATGAAGAGCAGTTTGTATTTTCTGAGCCGAAGATTCCAGTTAGTAAGAACCAGTATAAGAGAGAAATACCCGCCTCACCAACCCGTTTCCCAACACCTGCAAAAGCTAGAGCCCAGTGCCTGAGAGAAGCCCCGGTTAGAACACCAGGGAATTCCGCAGGAGCAGACACACTAACGACAGGTGTCATTAGCCCCGAGAGGAGGTGCCGTTCAATGGACGTGGATCTCAACCAGGCACACATGGAGGATACCCCGAAAAAGAAAGGAACCAATGTGTTTGGGAGCCTTGAGAGAGGACTGGATAAGGTTCTCACTGCGCTCACAAGGAACAAGAAGAAGGGCTCTGCCAGAGATGGACCAAGAAAGCGAAAGCTGCACTACAATGTGACTACAACTCGCCTGGTGAACCCCGACCAGCTCCTGAGCGAAATCATGGCTATTCTTCCAAAGAAGAACGTGGACTTCGTACAGAAAGGTTACACTCTAAAGTGTCAAACGCAGTCTGATTTTGGCAAAGTGACAATGCAGTTTGAACTGGAAGTGTGCCAGCTGCAGAGACCTGACGTGGTAGGCATCCGGAGACAGCGGCTGAAGGGTGATGCCTGGGTTTACAAGAGATTAGTGGAAGATATCTTGTCTGGCTGCAAGATGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKDYDELLKYYELYETIGTGGFAKVKLACHVLTGEMVAIKIMDKNALGSDLPRVKTEIDALKSLRHQHI
CQLYHVLETKNKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQILSAVAYVHSQGYAHRDLKPENLLFDENHKLKLIDFGLCAKPKGNKDYHLQTCC
GSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMRGKYEVPKWLSPSSILLLQQMLQVDPKKRISMRNLLNHPWVMQDYSC
PVEWQSKTPLTHLDEDCVTELSVHHRSSRQTMEDLISSWQYDHLTATYLLLLAKKARGKPARLQLPSFSCGTASTTPKSKNLSLEDMSTSDDNCVAGLID
YELCEDKLLAPKTPQVTKHLAESNHAASKSPAPGVRRAVANKLMDKENVCTPKSSVKNEEQFVFSEPKIPVSKNQYKREIPASPTRFPTPAKARAQCLRE
APVRTPGNSAGADTLTTGVISPERRCRSMDVDLNQAHMEDTPKKKGTNVFGSLERGLDKVLTALTRNKKKGSARDGPRKRKLHYNVTTTRLVNPDQLLSE
IMAILPKKNVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQRPDVVGIRRQRLKGDAWVYKRLVEDILSGCKM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50791
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * Changes compared with NM_010790.2 CDS:; g978c (silent); t1004c (residue change L335P) - common variant