cDNA Clone mouse MELK

• Synonyms: AI327312; mKIAA0175; MPK38
• Plasmid: pMEX3C mouse MELK
• Parent Plasmid: pMEX3C
• Expressed: MBP-mouse MELK
• Genbank: NM_010790.2 GI:31981625*
• Tag: MBP
• Species: Mouse

Sequence of Insert

GGATCCATGAAAGATTATGACGAACTCCTCAAATACTATGAACTATATGAAACGATTGGGACAGGTGGCTTTGCAAAGGTCAAACTGGCCTGCCATGTCCTCACTGGAGAGATGGTAGCTATAAAAATCATGGATAAGAATGCGCTAGGGAGTGATTTGCCCCGAGTCAAAACTGAGATCGATGCGCTGAAGAGTCTGAGACATCAGCACATATGTCAGCTCTACCATGTGCTGGAGACAAAGAACAAAATATTCATGGTTCTGGAGTACTGTCCAGGAGGAGAGCTGTTTGACTACATAATCTCCCAGGATCGCCTGTCGGAAGAGGAGACCCGGGTCGTCTTCCGTCAGATACTGTCTGCAGTTGCGTATGTCCACAGCCAGGGCTATGCCCACAGGGACCTCAAACCAGAAAATTTATTATTTGATGAAAATCATAAGCTAAAGCTGATTGACTTTGGTCTTTGTGCAAAACCCAAGGGCAACAAGGACTACCATCTGCAGACGTGCTGTGGGAGCCTTGCTTATGCAGCTCCTGAACTAATACAAGGGAAGTCGTACCTTGGATCAGAGGCAGATGTTTGGAGCATGGGCATCCTCCTGTATGTGCTCATGTGTGGATTTCTACCATTTGATGATGATAATGTCATGGCTTTGTACAAGAAGATAATGAGAGGGAAATACGAAGTTCCTAAGTGGCTCTCTCCCAGTAGCATTCTGCTTCTCCAGCAGATGTTGCAGGTGGACCCAAAGAAACGGATTTCTATGAGAAATCTCCTGAACCATCCCTGGGTCATGCAAGATTACAGCTGTCCCGTGGAGTGGCAAAGCAAGACTCCTTTGACTCACCTCGATGAGGATTGCGTGACAGAGCTTTCTGTACATCACCGCAGCAGCAGGCAGACAATGGAGGATTTAATTTCGTCGTGGCAGTACGATCACCTCACAGCCACCTACCTTCTGCTTCTAGCCAAGAAGGCCCGCGGGAAGCCGGCTCGTCTACAGCTCCCGTCCTTCTCTTGTGGAACCGCCAGCACCACTCCAAAGTCAAAGAATCTGAGCCTGGAAGATATGAGCACAAGTGATGATAACTGTGTGGCTGGATTGATAGACTATGAATTGTGTGAAGATAAATTATTAGCTCCCAAGACGCCACAGGTTACCAAACACTTGGCAGAATCAAATCACGCAGCATCTAAATCACCAGCGCCAGGGGTACGCAGAGCAGTGGCAAATAAATTAATGGACAAAGAAAATGTGTGCACTCCCAAGTCTTCTGTGAAGAATGAAGAGCAGTTTGTATTTTCTGAGCCGAAGATTCCAGTTAGTAAGAACCAGTATAAGAGAGAAATACCCGCCTCACCAACCCGTTTCCCAACACCTGCAAAAGCTAGAGCCCAGTGCCTGAGAGAAGCCCCGGTTAGAACACCAGGGAATTCCGCAGGAGCAGACACACTAACGACAGGTGTCATTAGCCCCGAGAGGAGGTGCCGTTCAATGGACGTGGATCTCAACCAGGCACACATGGAGGATACCCCGAAAAAGAAAGGAACCAATGTGTTTGGGAGCCTTGAGAGAGGACTGGATAAGGTTCTCACTGCGCTCACAAGGAACAAGAAGAAGGGCTCTGCCAGAGATGGACCAAGAAAGCGAAAGCTGCACTACAATGTGACTACAACTCGCCTGGTGAACCCCGACCAGCTCCTGAGCGAAATCATGGCTATTCTTCCAAAGAAGAACGTGGACTTCGTACAGAAAGGTTACACTCTAAAGTGTCAAACGCAGTCTGATTTTGGCAAAGTGACAATGCAGTTTGAACTGGAAGTGTGCCAGCTGCAGAGACCTGACGTGGTAGGCATCCGGAGACAGCGGCTGAAGGGTGATGCCTGGGTTTACAAGAGATTAGTGGAAGATATCTTGTCTGGCTGCAAGATGTGAGCGGCCGC

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQG
PLGSMKDYDELLKYYELYETIGTGGFAKVKLACHVLTGEMVAIKIMDKNALGSDLPRVKTEIDALKSLRHQHICQLYHVLETKNKIFMVLEYCPGGELFD
YIISQDRLSEEETRVVFRQILSAVAYVHSQGYAHRDLKPENLLFDENHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMG
ILLYVLMCGFLPFDDDNVMALYKKIMRGKYEVPKWLSPSSILLLQQMLQVDPKKRISMRNLLNHPWVMQDYSCPVEWQSKTPLTHLDEDCVTELSVHHRS
SRQTMEDLISSWQYDHLTATYLLLLAKKARGKPARLQLPSFSCGTASTTPKSKNLSLEDMSTSDDNCVAGLIDYELCEDKLLAPKTPQVTKHLAESNHAA
SKSPAPGVRRAVANKLMDKENVCTPKSSVKNEEQFVFSEPKIPVSKNQYKREIPASPTRFPTPAKARAQCLREAPVRTPGNSAGADTLTTGVISPERRCR
SMDVDLNQAHMEDTPKKKGTNVFGSLERGLDKVLTALTRNKKKGSARDGPRKRKLHYNVTTTRLVNPDQLLSEIMAILPKKNVDFVQKGYTLKCQTQSDF
GKVTMQFELEVCQLQRPDVVGIRRQRLKGDAWVYKRLVEDILSGCKM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50801
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * Changes compared with NM_010790.2 CDS:; g978c (silent); t1004c (residue change L335P) - common variant