cDNA Clone MYO6

• Plasmid: pCMV5 HALO6P2 MYO6
• Parent Plasmid: pCMV5 HALO6P2
• Expressed: HALO-MYO6
• Tag: HALO
• Species: Human

Sequence of Insert

GCGGCCGCGATGGAGGATGGAAAGCCCGTTTGGGCGCCACACCCTACAGATGGATTTCAGATGGGCAATATTGTGGATATTGGCCCCGACAGCTTAACAA TTGAACCCTTGAATCAGAAAGGCAAGACATTTTTGGCTCTCATAAACCAAGTGTTTCCTGCAGAAGAGGACAGTAAAAAAGATGTGGAAGATAACTGTTC ACTAATGTATTTAAATGAAGCCACACTGCTCCATAATATCAAAGTTCGATATAGTAAAGACAGAATTTATACATATGTCGCCAACATTCTGATTGCAGTG AATCCATACTTTGACATACCTAAAATATATTCTTCAGAAGCAATAAAGTCATATCAAGGAAAATCTCTTGGGACAAGACCACCTCATGTCTTTGCAATTG CTGATAAAGCTTTTCGAGACATGAAGGTGCTCAAGATGAGTCAGTCTATCATTGTATCTGGAGAATCAGGAGCCGGCAAAACAGAAAATACAAAATTTGT TCTAAGATACCTGACTGAATCCTATGGAACAGGTCAAGATATTGATGACAGAATTGTTGAAGCTAACCCACTCCTAGAAGCCTTTGGAAATGCGAAGACT GTTCGCAACAATAATAGCAGTCGATTTGGGAAATTTGTAGAAATACATTTTAATGAAAAGAGCTCAGTTGTTGGAGGATTTGTTTCACATTATCTCCTAG AGAAATCTAGGATCTGTGTTCAAGGCAAAGAGGAAAGAAATTATCATATCTTTTATAGGTTGTGTGCTGGTGCTTCTGAAGATATTAGAGAAAAACTTCA TTTGAGTTCACCAGATAATTTTCGGTATTTAAACCGAGGCTGCACTAGATACTTTGCTAACAAAGAAACTGACAAACAGATTTTACAGAACCGCAAAAGT CCTGAGTACCTTAAGGCAGGTTCTATGAAAGATCCTCTGCTAGATGACCATGGTGATTTTATTAGAATGTGCACGGCTATGAAAAAAATTGGTTTGGATG ATGAAGAAAAGCTTGATCTCTTCCGGGTAGTAGCTGGCGTCCTGCACCTTGGAAATATTGATTTTGAGGAAGCTGGCAGCACTTCAGGTGGTTGTAATCT GAAGAATAAATCTGCTCAGTCTTTGGAATATTGTGCTGAATTACTGGGTTTGGACCAAGATGATCTTCGAGTAAGTTTGACCACAAGAGTCATGCTAACA ACAGCAGGGGGCACCAAAGGAACAGTTATAAAGGTACCTCTGAAAGTGGAGCAAGCAAACAATGCTCGTGATGCCCTGGCAAAGACAGTGTATAGCCATC TTTTTGATCATGTGGTAAACAGAGTAAATCAGTGTTTTCCTTTTGAAACATCATCCTATTTTATTGGAGTCCTAGATATTGCTGGTTTTGAGTACTTTGA GCATAACAGTTTTGAACAATTTTGCATCAACTATTGCAATGAAAAACTTCAACAATTTTTTAATGAAAGGATTCTGAAGGAGGAACAAGAACTCTATCAA AAAGAAGGTTTAGGTGTTAATGAAGTGCATTATGTGGATAATCAGGACTGTATAGATTTAATTGAAGCCAAATTAGTGGGAATACTGGATATTTTGGATG AAGAAAATCGCCTTCCCCAGCCAAGTGATCAACACTTTACATCTGCAGTTCACCAAAAGCACAAGGATCATTTTCGACTCACTATTCCCAGAAAATCTAA GCTGGCAGTTCATAGGAATATCAGAGACGACGAAGGCTTCATTATCAGGCATTTTGCGGGGGCAGTGTGCTATGAAACAACCCAGTTTGTGGAGAAAAAT AATGATGCTTTACATATGTCTCTTGAATCCTTAATATGTGAATCCAGAGATAAGTTTATACGGGAATTATTTGAATCATCCACAAATAACAACAAAGATA CTAAACAAAAAGCAGGAAAACTTAGCTTCATCAGCGTGGGAAACAAGTTTAAGACACAGTTAAATTTGCTTCTGGATAAACTTCGAAGTACTGGAGCAAG CTTTATTCGTTGCATCAAACCTAACTTAAAGATGACAAGCCACCACTTTGAAGGTGCTCAAATTCTGTCTCAGCTTCAGTGTTCAGGGATGGTGTCTGTT TTGGACTTGATGCAGGGTGGTTACCCATCACGAGCTTCATTTCATGAACTCTACAACATGTACAAAAAGTATATGCCAGATAAACTTGCAAGATTGGATC CAAGACTATTTTGTAAGGCTTTGTTTAAAGCTTTGGGCTTAAATGAAAATGACTACAAGTTTGGGTTAACCAAAGTATTTTTTAGACCTGGCAAGTTTGC AGAATTTGATCAGATCATGAAGTCTGACCCTGACCACTTAGCAGAGTTGGTTAAAAGAGTCAATCACTGGCTCACATGCAGTCGCTGGAAGAAAGTTCAG TGGTGCTCACTCTCAGTCATCAAATTGAAAAACAAAATAAAATATCGAGCTGAAGCCTGCATTAAAATGCAAAAAACTATTCGAATGTGGCTTTGCAAGA GGAGACACAAACCTCGCATTGATGGTCTGGTTAAGGTGGGCACACTGAAAAAACGACTTGATAAATTTAATGAGGTAGTCAGTGTGTTGAAAGATGGAAA ACCCGAGATGAATAAACAGATCAAGAATCTGGAAATTTCTATTGATACTTTGATGGCCAAAATTAAGTCCACTATGATGACGCAGGAACAAATCCAGAAA GAATATGATGCACTGGTTAAAAGCTCAGAGGAACTCCTCAGTGCATTACAGAAAAAAAAACAGCAGGAAGAGGAAGCAGAAAGGCTGAGGCGTATTCAAG AAGAAATGGAAAAGGAAAGAAAAAGACGTGAAGAAGACGAAAAACGTCGAAGAAAGGAAGAGGAGGAAAGGCGGATGAAACTTGAGATGGAAGCAAAGAG AAAACAAGAAGAAGAAGAGAGAAAGAAAAGGGAAGATGATGAAAAACGCATTCAAGCTGAAGTGGAGGCACAGCTGGCCCGACAGAAGGAGGAGGAATCC CAACAGCAAGCAGTTCTGGAGCAGGAGCGCAGGGACCGGGAGCTGGCCCTGAGGATTGCCCAGAGTGAAGCCGAGCTCATCAGTGATGAGGCCCAGGCCG ACCTGGCGCTGCGGAGCTTGGATTCCTATCCGGTAACTTCTAAAAATGATGGAACAAGACCCAAAATGACACCGGAACAAATGGCCAAAGAAATGTCAGA ATTTTTGAGTAGAGGTCCTGCTGTACTAGCCACCAAAGCAGCTGCTGGTACTAAGAAATATGATCTTAGTAAATGGAAATATGCAGAACTACGTGATACC ATCAATACTTCTTGTGATATTGAGCTCCTGGCAGCTTGCAGAGAAGAATTTCATAGGAGACTAAAAGTGTATCATGCTTGGAAATCTAAGAACAAGAAGA GAAATACTGAAACAGAGCAACGTGCTCCAAAGTCTGTTACTGATTATGCTCAGCAAAACCCAGCAGCTCAGATTCCTGCCAGGCAGCGGGAGATTGAAAT GAACCGACAGCAACGCTTCTTCCGCATCCCATTCATCCGCCCTGCCGACCAGTACAAAGACCCTCAGAGTAAGAAAAAAGGCTGGTGGTATGCCCATTTT GATGGACCATGGATTGCCCGGCAAATGGAACTCCATCCTGACAAGCCACCCATCCTACTTGTGGCTGGTAAGGACGACATGGAGATGTGTGAGCTGAATC TTGAGGAGACTGGCCTGACTCGGAAGCGTGGTGCTGAGATCTTGCCAAGACAGTTTGAAGAAATCTGGGAACGCTGTGGAGGCATCCAGTACCTTCAGAA TGCGATTGAGAGCAGACAGGCTCGGCCCACCTATGCAACAGCCATGCTGCAGAGTCTGTTAAAGTAGGCGGCCGC

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFKEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGLEV
LFQGPGSAGIPGSTRAAAMEDGKPVWAPHPTDGFQMGNIVDIGPDSLTIEPLNQKGKTFLALINQVFPAEEDSKKDVEDNCSLMYLNEATLLHNIKVRYS
KDRIYTYVANILIAVNPYFDIPKIYSSEAIKSYQGKSLGTRPPHVFAIADKAFRDMKVLKMSQSIIVSGESGAGKTENTKFVLRYLTESYGTGQDIDDRI
VEANPLLEAFGNAKTVRNNNSSRFGKFVEIHFNEKSSVVGGFVSHYLLEKSRICVQGKEERNYHIFYRLCAGASEDIREKLHLSSPDNFRYLNRGCTRYF
ANKETDKQILQNRKSPEYLKAGSMKDPLLDDHGDFIRMCTAMKKIGLDDEEKLDLFRVVAGVLHLGNIDFEEAGSTSGGCNLKNKSAQSLEYCAELLGLD
QDDLRVSLTTRVMLTTAGGTKGTVIKVPLKVEQANNARDALAKTVYSHLFDHVVNRVNQCFPFETSSYFIGVLDIAGFEYFEHNSFEQFCINYCNEKLQQ
FFNERILKEEQELYQKEGLGVNEVHYVDNQDCIDLIEAKLVGILDILDEENRLPQPSDQHFTSAVHQKHKDHFRLTIPRKSKLAVHRNIRDDEGFIIRHF
AGAVCYETTQFVEKNNDALHMSLESLICESRDKFIRELFESSTNNNKDTKQKAGKLSFISVGNKFKTQLNLLLDKLRSTGASFIRCIKPNLKMTSHHFEG
AQILSQLQCSGMVSVLDLMQGGYPSRASFHELYNMYKKYMPDKLARLDPRLFCKALFKALGLNENDYKFGLTKVFFRPGKFAEFDQIMKSDPDHLAELVK
RVNHWLTCSRWKKVQWCSLSVIKLKNKIKYRAEACIKMQKTIRMWLCKRRHKPRIDGLVKVGTLKKRLDKFNEVVSVLKDGKPEMNKQIKNLEISIDTLM
AKIKSTMMTQEQIQKEYDALVKSSEELLSALQKKKQQEEEAERLRRIQEEMEKERKRREEDEKRRRKEEEERRMKLEMEAKRKQEEEERKKREDDEKRIQ
AEVEAQLARQKEEESQQQAVLEQERRDRELALRIAQSEAELISDEAQADLALRSLDSYPVTSKNDGTRPKMTPEQMAKEMSEFLSRGPAVLATKAAAGTK
KYDLSKWKYAELRDTINTSCDIELLAACREEFHRRLKVYHAWKSKNKKRNTETEQRAPKSVTDYAQQNPAAQIPARQREIEMNRQQRFFRIPFIRPADQY
KDPQSKKKGWWYAHFDGPWIARQMELHPDKPPILLVAGKDDMEMCELNLEETGLTRKRGAEILPRQFEEIWERCGGIQYLQNAIESRQARPTYATAMLQS
LLK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU51676
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee