cDNA Clone FANCL/UBE2T

• Plasmid: pET15 6His smt3 codon optimised FANCL tv2 Q109-H375-Linker-UBE2T
• Parent Plasmid: pET15 6His thr smt3
• Expressed: 6His smt3 codon optimised FANCL tv2 Q109-H375-Linker-UBE2T
• Genbank: See Notes
• Tag: 6His
• Tag: SUMO
• Species: Human

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPED
LDMEDNDIIEAHREQIGGQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQF
LAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLEI
DFPARAILEKSDFTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCSKPITLKMSGRKHTGSTGSTGETGYTQG
MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRP
SLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV*

• Antibiotic: Amp
• DU Number: DU50950
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Smt3 (yeast sumo): NM_001180818.1 GI:296143849; FANCL: codon optimised NP_001108108.1; UBE2T: NM_014176.3 GI:209969667; FANCL fragment cloned in BglII/KpnI, followed by Linker-UBE2T into NotI and removal of extra residue after smt3 removed by mutagenesis