cDNA Clone FAM83G

• Plasmid: pGEX6P-1 FAM83G D262A-6His
• Parent Plasmid: pGEX6P-1
• Expressed: GST-FAM83G D262A-6His
• Genbank: NM_001039999.2
• Tag: GST
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAACCATGTGAA
CTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCGAGGAGCAGC
GGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAG
GTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAA
GCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTC
GGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGC
GAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCC
CTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGG
ACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGC
GTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGT
GCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACA
TGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCCGGCTTCAAG
AGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTT
CCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCAAGAATC
TCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACC
AAGTTCAAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGCTGGAGACCG
GGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACC
GGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTTGACCGG
CAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGAGCCTCAAGGGCAT
CCCTATGGAGAAGGAACCGGAGCCGGAGCCTATTGTGCTGCCCTCTGTGG
TCCCCCTGGTGCCCGCGGGCACTGTGGCCAAGAAGCTCGTCAACCCCAAG
TACGCACTTGTCAAGGCCAAGAGCGTCGACGAGATTGCCAAGATCTCCTC
TGAGAAGCAGGAGGCCAAGAAGCCCCTGGGGCTGAAAGGCCCAGCGCTGG
CTGAGCATCCAGGGGAACTCCCCGAGCTGCTGCCACCCATCCACCCAGGA
CTGCTTCACCTGGAGAGGGCCAACATGTTTGAGTACCTGCCCACGTGGGT
GGAGCCAGACCCGGAGCCTGGCAGCGACATCCTGGGCTACATCAATATCA
TCGACCCCAACATCTGGAACCCCCAGCCCAGCCAGATGAACCGCATCAAG
ATCCGTGACACCTCCCAGGCCAGCGCCCAGCACCAGCTGTGGAAGCAGAG
CCAGGACAGCAGGCCCCGTCCAGAGCCTTGCCCTCCCCCAGAGCCCAGTG
CCCCCCAGGACGGTGTCCCAGCTGAGAACGGCCTCCCCCAGGGGGACCCT
GAGCCATTGCCCCCCGTGCCCAAGCCCCGGACAGTCCCTGTGGCAGATGT
ACTAGCCCGGGACAGCAGTGATATTGGCTGGGTCCTGGAGCTCCCCAAAG
AGGAAGCTCCCCAGAATGGGACAGACCATAGGCTACCCAGGATGGCAGGC
CCAGGCCACGCCCCACTCCAGCGGCAGCTATCTGTGACCCAGGATGACCC
CGAGAGCCTCGGGGTGGGGCTCCCCAATGGGCTGGATGGGGTGGAAGAAG
AAGATGATGACGACTACGTAACCCTCAGTGACCAGGACAGCCACTCAGGC
AGCTCCGGCCGTGGCCCTGGCCCCCGACGGCCCTCAGTGGCTTCCTCTGT
GTCAGAGGAGTACTTCGAGGTGAGAGAGCACTCAGTCCCTCTCCGGAGGC
GCCACTCAGAGCAAGTGGCCAACGGGCCAACCCCACCACCGCGCCGGCAG
CTGAGTGCCCCCCATATAACCCGAGGGACCTTTGTTGGACCCCAGGGTGG
CTCCCCATGGGCCCAGAGTCGGGGAAGAGAAGAAGCAGATGCGTTGAAGA
GGATGCAGGCCCAGCGCTCCACAGACAAGGAGGCACAGGGCCAGCAGTTT
CATCATCACAGGGTCCCTGCCTCAGGGACTAGGGATAAAGACGGCTTCCC
AGGACCCCCTAGGTACCGCTCTGCTGCTGACAGCGTCCAGAGCTCTACCA
GAAACGCTGGCCCAGCCATGGCTGGCCCCCACCACTGGCAGGCCAAGGGA
GGTCAGGTACCCCGCCTGCTTCCGGACCCCGGCAGCCCAAGACTGGCCCA
AAATGCCCGCCCCATGACCGATGGCAGGGCCACCGAGGAGCATCCGAGTC
CCTTCGGAATCCCATACTCCAAACTGTCTCAGTCGAAGCACCTAAAGGCC
AGGACGGGCGGTAGCCAGTGGGCCTCATCGGATTCTAAACGGAGGGCTCA
AGCCCCCCGGGACCGCAAAGACCCCCATCACCATCACCATCACTAGGCGG
CCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAFSQVQCLDDNHVNWRSS
ESKPEFFYSEEQRLALEALVARGRDAFYEVLKRENIRDFLSELELKRILE
TIEVYDPGSEDPRGTGPSQGPEDNGVGDGEEASGADGVPIEAEPLPSLEY
WPQKSDRSIPQLDLGWPDTIAYRGVTRASVYMQPPIDGQAHIKEVVRKMI
SQAQKVIAVVMDMFTDVDIFKDLLDAGFKRKVAVYIIVDESNVKYFLHMC
ERACMHLGHLKNLRVRSSGGTEFFTRSATKFKGALAQKFMFVAGDRAVCG
SYSFTWSAARTDRNVISVLSGQVVEMFDRQFQELYLMSHSVSLKGIPMEK
EPEPEPIVLPSVVPLVPAGTVAKKLVNPKYALVKAKSVDEIAKISSEKQE
AKKPLGLKGPALAEHPGELPELLPPIHPGLLHLERANMFEYLPTWVEPDP
EPGSDILGYINIIDPNIWNPQPSQMNRIKIRDTSQASAQHQLWKQSQDSR
PRPEPCPPPEPSAPQDGVPAENGLPQGDPEPLPPVPKPRTVPVADVLARD
SSDIGWVLELPKEEAPQNGTDHRLPRMAGPGHAPLQRQLSVTQDDPESLG
VGLPNGLDGVEEEDDDDYVTLSDQDSHSGSSGRGPGPRRPSVASSVSEEY
FEVREHSVPLRRRHSEQVANGPTPPPRRQLSAPHITRGTFVGPQGGSPWA
QSRGREEADALKRMQAQRSTDKEAQGQQFHHHRVPASGTRDKDGFPGPPR
YRSAADSVQSSTRNAGPAMAGPHHWQAKGGQVPRLLPDPGSPRLAQNARP
MTDGRATEEHPSPFGIPYSKLSQSKHLKARTGGSQWASSDSKRRAQAPRD
RKDPHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28259
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: internal BamHI killed T2271C silent change