cDNA Clone Brd3 (ms)

• Plasmid: pCAGGS 3xFLAG ms Brd3
• Expressed: FLAG-mouse Brd3
• Tag: 3FLAG
• Species: Mouse

Sequence of Insert

agatctATGTCCACTACAGCGGCTGCCCCCACGGGGATCCCGGCAGTCCCGGGCCCCGTGAACCCTCCCCCACCTGAGGTCTCCAACCCCAGCAAGCCCGGGCGAAAGACTAACCAACTGCAGTACATGCAGAATGTAGTGGTGAAGACACTCTGGAAACATCAGTTTGCCTGGCCTTTCTACCAGCCTGTGGATGCAATCAAGCTGAACCTGCCTGATTATCATAAAATAATAAAAAACCCAATGGACATGGGGACTATCAAGAAGAGACTAGAAAATAATTATTATTGGAGTGCCAGTGAGTGTATGCAGGACTTCAACACCATGTTTACAAACTGTTATATTTATAATAAGCCCACAGATGATATAGTGCTAATGGCCCAGGCCTTAGAGAAAATCTTTCTGCAGAAAGTGGCCCAGATGCCTCAGGAGGAAGTTGAACTATTGCCCCCTGCTCCAAAGGGCAAAGGCCGGAAGCCAGCTGCAGGAGCCCAAAATGCAGGTTCCCAACAAGTGGCAGCTGTGTCTTCGGTCTCCCCAGCAACCCCCTTCCAGAACATACCCCCCACCGTGTCCCAGACACCCGTCATTGCCGCTACCCCTGTACCAACCATCACTGCAAACGTCACGTCAGTTCCAGTCCCCCCACCCGCTGCACCGCCTCCTCCTGCGACACCCATCGTCCCTGTGGTCCCTCCCACACCGCCTGTAGTCAAGAAAAAGGGCGTGAAGCGGAAAGCAGACACAACCACACCCACAACATCTGCCATCACTGCCAGCCGGAGTGAGTCTCCCCCACCACTTTCAGAGCCCAAGCAAGCCAAGGTAGTGGCCCGAAGGGAGAGCGGGGGCCGCCCCATCAAACCTCCCAAGAAGGACCTGGAAGATGGTGAGGTCCCACAGCACGCGGGTAAAAAGGGAAAACTGTCCGAGCACCTGCGGCACTGTGACAGCATCCTCCGGGAGATGCTGTCCAAGAAGCATGCTGCCTATGCGTGGCCCTTTTACAAGCCAGTGGACGCGGAGGCGCTGGAGCTGCATGACTACCATGACATCATCAAGCACCCCATGGACCTCAGCACGGTCAAAAGGAAGATGGATAGCCGAGAGTACCCAGATGCACAGGGCTTTGCTGCTGATATCCGGTTAATGTTCTCGAATTGTTATAAGTACAACCCTCCAGACCACGAAGTGGTGGCCATGGCCAGGAAGCTCCAGGATGTGTTTGAGATGAGGTTTGCCAAGATGCCCGATGAGCCCATGGAGGCGCCTGCGCTGCCCGCTCCCACGGCCCCCATCGTGAGCAAGGGGGCTGAGAGCAGCCGCAGTAGTGAGGAGAGCTCTTCAGATTCAGGCAGCTCAGACTCAGAAGAGGAGCGAGCCACTCGGCTGGCTGAGCTGCAGGAGCAGCTGAAGGCCGTGCACGAACAGCTGGCTGCCTTGTCTCAGGCCCCAGTGAACAAACCAAAGAAGAAGAAAGAGAAGAAGGAAAAGGAAAAGAAGAAGAAAGACAAGGACAAAGACAAAGAGAAGGAGAAGCACAAGGCCAAGTCTGAGGAGGAGAAGAAGGCCAAGGCAGCCCCAGCAGCCAAGCAGGCCCAGCAGAAAAAGGCTCCCACCAAGAAGGCCAACAGCACAACCACAGCCAGCAGACAGCTCAAGAAGGGCGGCAAGCAGGCATCTGCGTCCTATGACTCAGAGGAAGAGGAGGAGGGCCTGCCCATGAGCTATGATGAAAAGCGACAACTTAGCCTTGACATCAACCGGCTGCCCGGCGAGAAGCTAGGGCGTGTGGTGCACATCATTCAGTCTCGGGAGCCCTCGCTTCGGGACTCAAACCCAGACGAGATTGAGATTGACTTTGAGACCCTGAAGCCAACCACGCTGCGGGAACTGGAGAGATATGTCAAGTCTTGTTTACAAAAAAAGCAGAGGAAACCATTGTCAACAAGCGGGAAGAAGCAGGCAGCCAAATCGAAAGAGGAGCTAGCTCAGGAGAAGAAAAAGGAGCTGGAGAAGCGGCTGCAGGATGTCAGTGGACAACTGAACAGCAAAAAACCCACTAAGAAAGAGAAGTCCGGCTCAGCTCCCTCAGGAGGCCCATCAAGGCTCAGCAGTAGCAGCTCCTCTGAGTCTGCGAGCAGTAGCTCCAGTGGATCGAGCTCCGACAGCAGTGACTCAGAGTGAGCGGCCGC

Amino Acid Sequence

MDYKDHDGDYKDHDIDYKDDDDKGSMSTTAAAPTGIPAVPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQNAGSQQVAAVSSVSPATPFQNIPPTVSQTPVIAATPVPTITANVTSVPVPPPAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSEPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRHCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDSREYPDAQGFAADIRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPMEAPALPAPTAPIVSKGAESSRSSEESSSDSGSSDSEEERATRLAELQEQLKAVHEQLAALSQAPVNKPKKKKEKKEKEKKKKDKDKDKEKEKHKAKSEEEKKAKAAPAAKQAQQKKAPTKKANSTTTASRQLKKGGKQASASYDSEEEEEGLPMSYDEKRQLSLDINRLPGEKLGRVVHIIQSREPSLRDSNPDEIEIDFETLKPTTLRELERYVKSCLQKKQRKPLSTSGKKQAAKSKEELAQEKKKELEKRLQDVSGQLNSKKPTKKEKSGSAPSGGPSRLSSSSSSESASSSSSGSSSDSSDSE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55084
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Could you please clone mouse Brd3 into pCAGGs with an N-terminal FLAG tag? DU46362 might be used as template.