cDNA Clone FAM83G

• Plasmid: pCS2+ FAM83G S614A
• Parent Plasmid: pCS2+
• Expressed: FAM83G S614A
• Genbank: NM_001039999.2
• Species: Human

Sequence of Insert

GGATCCATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAACCATGTGAA
CTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCGAGGAGCAGC
GGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAG
GTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAA
GCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTC
GGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGC
GAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCC
CTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGG
ACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGC
GTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGT
GCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACA
TGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCCGGCTTCAAG
AGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTT
CCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCAAGAATC
TCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACC
AAGTTCAAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGATGGAGACCG
GGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACC
GGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTTGACCGG
CAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGAGCCTCAAGGGCAT
CCCTATGGAGAAGGAACCGGAGCCGGAGCCTATTGTGCTGCCCTCTGTGG
TCCCCCTGGTGCCCGCGGGCACTGTGGCCAAGAAGCTCGTCAACCCCAAG
TACGCACTTGTCAAGGCCAAGAGCGTCGACGAGATTGCCAAGATCTCCTC
TGAGAAGCAGGAGGCCAAGAAGCCCCTGGGGCTGAAAGGCCCAGCGCTGG
CTGAGCATCCAGGGGAACTCCCCGAGCTGCTGCCACCCATCCACCCAGGA
CTGCTTCACCTGGAGAGGGCCAACATGTTTGAGTACCTGCCCACGTGGGT
GGAGCCAGACCCGGAGCCTGGCAGCGACATCCTGGGCTACATCAATATCA
TCGACCCCAACATCTGGAACCCCCAGCCCAGCCAGATGAACCGCATCAAG
ATCCGTGACACCTCCCAGGCCAGCGCCCAGCACCAGCTGTGGAAGCAGAG
CCAGGACAGCAGGCCCCGTCCAGAGCCTTGCCCTCCCCCAGAGCCCAGTG
CCCCCCAGGACGGTGTCCCAGCTGAGAACGGCCTCCCCCAGGGGGACCCT
GAGCCATTGCCCCCCGTGCCCAAGCCCCGGACAGTCCCTGTGGCAGATGT
ACTAGCCCGGGACAGCAGTGATATTGGCTGGGTCCTGGAGCTCCCCAAAG
AGGAAGCTCCCCAGAATGGGACAGACCATAGGCTACCCAGGATGGCAGGC
CCAGGCCACGCCCCACTCCAGCGGCAGCTATCTGTGACCCAGGATGACCC
CGAGAGCCTCGGGGTGGGGCTCCCCAATGGGCTGGATGGGGTGGAAGAAG
AAGATGATGACGACTACGTAACCCTCAGTGACCAGGACAGCCACTCAGGC
AGCTCCGGCCGTGGCCCTGGCCCCCGACGGCCCTCAGTGGCTTCCgCTGT
GTCAGAGGAGTACTTCGAGGTGAGAGAGCACTCAGTCCCTCTCCGGAGGC
GCCACTCAGAGCAAGTGGCCAACGGGCCAACCCCACCACCGCGCCGGCAG
CTGAGTGCCCCCCATATAACCCGAGGGACCTTTGTTGGACCCCAGGGTGG
CTCCCCATGGGCCCAGAGTCGGGGAAGAGAAGAAGCAGATGCGTTGAAGA
GGATGCAGGCCCAGCGCTCCACAGACAAGGAGGCACAGGGCCAGCAGTTT
CATCATCACAGGGTCCCTGCCTCAGGGACTAGGGATAAAGACGGCTTCCC
AGGACCCCCTAGGTACCGCTCTGCTGCTGACAGCGTCCAGAGCTCTACCA
GAAACGCTGGCCCAGCCATGGCTGGCCCCCACCACTGGCAGGCCAAGGGA
GGTCAGGTACCCCGCCTGCTTCCGGACCCCGGCAGCCCAAGACTGGCCCA
AAATGCCCGCCCCATGACCGATGGCAGGGCCACCGAGGAGCATCCGAGTC
CCTTCGGAATCCCATACTCCAAACTGTCTCAGTCGAAGCACCTAAAGGCC
AGGACGGGCGGTAGCCAGTGGGCCTCATCGGATTCTAAACGGAGGGCTCA
AGCCCCCCGGGACCGCAAAGACCCCTAGGCGGCCGC

Amino Acid Sequence

MAFSQVQCLDDNHVNWRSSESKPEFFYSEEQRLALEALVARGRDAFYEVL
KRENIRDFLSELELKRILETIEVYDPGSEDPRGTGPSQGPEDNGVGDGEE
ASGADGVPIEAEPLPSLEYWPQKSDRSIPQLDLGWPDTIAYRGVTRASVY
MQPPIDGQAHIKEVVRKMISQAQKVIAVVMDMFTDVDIFKDLLDAGFKRK
VAVYIIVDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFTRSATKF
KGALAQKFMFVDGDRAVCGSYSFTWSAARTDRNVISVLSGQVVEMFDRQF
QELYLMSHSVSLKGIPMEKEPEPEPIVLPSVVPLVPAGTVAKKLVNPKYA
LVKAKSVDEIAKISSEKQEAKKPLGLKGPALAEHPGELPELLPPIHPGLL
HLERANMFEYLPTWVEPDPEPGSDILGYINIIDPNIWNPQPSQMNRIKIR
DTSQASAQHQLWKQSQDSRPRPEPCPPPEPSAPQDGVPAENGLPQGDPEP
LPPVPKPRTVPVADVLARDSSDIGWVLELPKEEAPQNGTDHRLPRMAGPG
HAPLQRQLSVTQDDPESLGVGLPNGLDGVEEEDDDDYVTLSDQDSHSGSS
GRGPGPRRPSVASAVSEEYFEVREHSVPLRRRHSEQVANGPTPPPRRQLS
APHITRGTFVGPQGGSPWAQSRGREEADALKRMQAQRSTDKEAQGQQFHH
HRVPASGTRDKDGFPGPPRYRSAADSVQSSTRNAGPAMAGPHHWQAKGGQ
VPRLLPDPGSPRLAQNARPMTDGRATEEHPSPFGIPYSKLSQSKHLKART
GGSQWASSDSKRRAQAPRDRKDP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28374
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: internal BamHI killed T2271C silent change. Parental modified pCS2+ 28149