cDNA Clone VPS13C

• Plasmid: pMEX VPS13C (residues 1-300)
• Parent Plasmid: pMEX-3C
• Expressed: MBP-VPS13C (residues 1-300)
• Genbank: Q709C8
• Tag: MBP

Sequence of Insert

GGATCCATGGTGCTGGAGTCGGTGGTCGCGGACTTGCTGAACCGCTTCCTGGGGGACTATGTGGAGAACCTGAACAAGTCCCAGCTGAAGCTGGGCATCTGGGGCGGAAATGTGGCTTTAGATAATCTACAGATAAAAGAAAATGCCCTGAGTGAATTGGATGTTCCTTTTAAAGTCAAGGCTGGCCAAATTGATAAATTAACTTTGAAGATTCCTTGGAAGAACCTTTATGGAGAAGCAGTTGTTGCGACCCTGGAAGGATTATACCTGCTTGTTGTCCCTGGAGCAAGTATTAAGTATGATGCTGTAAAAGAAGAAAAATCCTTGCAGGATGTTAAACAGAAAGAGCTATCCCGAATTGAAGAAGCCCTTCAAAAAGCAGCAGAAAAAGATAAGCCAAAAGAAGCCAAAAAGGATACATTTGTGGAAAAATTGGCAACTCAAGTAATAAAAAATGTACAAGTAAAAATCACAGATATTCACATTAAATATGAAGATGATGTCACTGATCCAAAGCGGCCTCTTTCATTTGGTGTCACACTGGGAGAGCTTAGTCTACTGACTGCAAATGAACACTGGACTCCATGCATATTAAATGAAGCAGACAAAATTATATACAAGCTTATACGACTTGATAGTCTTAGCGCCTACTGGAATGTAAATTGCAGCATGTCTTACCAGAGATCAAGGGAACAGATTTTGGATCAGCTGAAAAATGAAATTCTTACAAGTGGAAATATACCCCCAAATTATCAATACATTTTCCAGCCAATATCAGCCTCTGCAAAACTCTACATGAATCCTTATGCAGAATCAGAGCTCAAAACGCCCAAACTGGATTGCAACATAGAAATACAAAATATTGCCATTGAACTGACCAAACCTCAGTACTTAAGTATGATTGACTAAGCGGCCGC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPLGSMVLESVVADLLNRFLGDYVENLNKSQLKLGIWGGNVALDNLQIKENALSELDVPFKVKAGQIDKLTLKIPWKNLYGEAVVATLEGLYLLVVPGASIKYDAVKEEKSLQDVKQKELSRIEEALQKAAEKDKPKEAKKDTFVEKLATQVIKNVQVKITDIHIKYEDDVTDPKRPLSFGVTLGELSLLTANEHWTPCILNEADKIIYKLIRLDSLSAYWNVNCSMSYQRSREQILDQLKNEILTSGNIPPNYQYIFQPISASAKLYMNPYAESELKTPKLDCNIEIQNIAIELTKPQYLSMID*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU55302
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Review pilot expression before deciding on antibody