cDNA Clone Fis1

• Plasmid: pBABE hygro mCherry Fis1 101-152
• Parent Plasmid: pBABE hygro mCherry DU
• Expressed: mCherry-Fis1 101-152
• Genbank: xxxxx
• Tag: mCherry

Sequence of Insert

GGATCCGAGCCCCAGAACAACCAGGCCAAGGAACTGGAGCGGCTCATTGACAAGGCCATGAAGAAAGATGGACTCGTGGGCATGGCCATCGTGGGAGGCATGGCCCTGGGTGTGGCGGGACTGGCCGGACTCATCGGACTTGCTGTGTCCAAGTCCAAATCCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKGSEPQNNQAKELERLIDKAMKKDGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55314
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: please clone into pbabe hygo (construct similar to DU40799, but without GFP), source material 35734 has stop out of frame which was corrected with SW3205/6
• Low Temp: No