cDNA Clone CUL2 (elegans)

• Plasmid: pMEX3C CUL2 (elegans) 569-790
• Parent Plasmid: pMEX3C
• Expressed: MBP CUL2 (elegans) 569-790
• Genbank: NP_499825.3
• Tag: MBP
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGTTCACCGATATCGGGCTTAGCCAGGAGCTATCGAACAACTT
TGATAAGCACATTGCGGACATTAAAACTGTGCAGCCCGACGTGAAGTTTG
TGCCGACACAGACGATGATTCTGCAGGCAGGTAGCTGGCCGCTCAACGCT
CCACAGCTCTCTACGAATTCGAATAATCAGACCGCACAAGACGTGGCCAA
CTTCCATCTGCCACGTATCCTGCAGCCTGTGATTCAGGAGTTTGAGAAGT
TCTACACTGGGAAGCACAACGGGCGAAAACTGACATGGCTTTTCAATATG
TCACAGGGAGATGTCAGGCTTACATACCTCGATAAGCAGTATGTCGCACA
GATGTATGTCTATCAGATGGCAGCGCTGCTTTGCTTTGAACGTCGCGATG
CTATCCTCGTTAAGGACATCGGAGAGGAGATTGGTGTGAGCGGTGACTAT
CTGCTAAAGACGATTCGAACGATTTTGGACGTTACCCTGCTGACGTGTGA
TGATCAGAATCTAACGGCCGACTCGTTGGTTCGTCTGAACATGTCGATGA
CGTCGAAACGGATGAAGTTCCGGCTTCAGGCGCCACAAGTGAATAAGGCT
GTCGAGAAGGAGCAGGAGGCCGTCGCGAATACTGTTTCACAAGACCGAAA
GTACTATATGGAGTGCGCAATTTAAGCGGCCGC

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFP
QVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALM
FNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLP
TFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVI
NAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQG
PLGSMFTDIGLSQELSNNFDKHIADIKTVQPDVKFVPTQTMILQAGSWPL
NAPQLSTNSNNQTAQDVANFHLPRILQPVIQEFEKFYTGKHNGRKLTWLF
NMSQGDVRLTYLDKQYVAQMYVYQMAALLCFERRDAILVKDIGEEIGVSG
DYLLKTIRTILDVTLLTCDDQNLTADSLVRLNMSMTSKRMKFRLQAPQVN
KAVEKEQEAVANTVSQDRKYYMECAI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51936
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct to express C. elegans Cullin 2 (569-790), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.