cDNA Clone mouse Pum1

• Synonyms: AA517475; mKIAA0099; Pumm
• Plasmid: pGEX6P1 mouse Pum1
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C Mouse Pum1
• Genbank: NM_030722.2 GI:227430379
• Tag: GST
• Species: Mouse

Sequence of Insert

AGATCTATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACCAGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAGCCAGCTGCAAATCAGGCTCTTGCAGCTGGAACACACTCCAGCCCTGTCCCAGGATCCATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTGGGGGAGGAGGAAGTGGTGGAGGCGGCTATAATACTAGCAAACATCGATGGCCTACAGGGGATAACATTCATGCAGAACACCAGGTACGCTCTATGGATGAATTGAATCATGATTTTCAAGCACTTGCTCTGGAAGGGAGAGCTATGGGCGAGCAGCTCTTGCCAGGTAAAAAGTTTTGGGAAACAGATGAATCCAGCAAAGATGGACCAAAAGGAATATTCCTGGGTGATCAATGGCGAGACAGTGCCTGGGGAACATCAGATCATTCAGTTTCCCAGCCAATCATGGTGCAGAGAAGACCTGGTCAGAGTTTCCATGTGAACAGTGAGGTCAATTCTGTACTGTCCCCACGGTCGGAGAGTGGGGGACTAGGCGTTAGCATGGTGGAGTATGTGTTGAGCTCATCCCCGGGCGACTCCTGTCTAAGAAAAGGAGGATTTGGCCCAAGGGATGCAGACAGTGATGAAAACGACAAAGGTGAAAAGAAGAACAAGGGTACGTTTGATGGAGATAAGCTAGGAGATTTGAAGGAGGAGGGAGATGTGATGGACAAGACCAATGGTTTGCCAGTGCAGAATGGGATTGATGCAGACGTCAAAGATTTTAGCCGTACCCCTGGTAATTGCCAGAACTCTGCTAATGAAGTGGATCTTCTGGGTCCAAACCAGAATGGTTCTGAGGGCTTAGCCCAGCTGACCAGCACCAATGGTGCCAAGCCTGTGGAGGATTTCTCCAACATGGAGTCCCAGAGTGTCCCCTTGGACCCCATGGAACATGTGGGCATGGAGCCTCTGCAGTTTGATTATTCAGGCACGCAGGTACCTGTGGACTCTGCAGCAGCAACTGTGGGACTTTTTGACTACAATTCTCAACAACAGCTATTCCAAAGACCCAATGCACTTGCTGTTCAGCAGTTGACAGCTGCCCAGCAGCAGCAGTATGCACTTGCAGCAGCCCACCAGCCTCACATCGCAGGTTTAGCTCCCGCTGCGTTTGTCCCCAACCCATACATCATCAGCGCTGCTCCCCCAGGGACGGACCCCTACACAGCTGGATTGGCTGCAGCAGCGACACTCGGCCCAGCTGTGGTCCCTCACCAATATTATGGAGTCACGCCATGGGGTGTCTACCCTGCCAGTCTCTTCCAGCAGCAGGCAGCTGCAGCTGCAGCAGCTACAAACTCTGCTACTCAGCAGAGCGCTCCGCAGGCCCAGCAGGGACAGCAGCAGGTTCTTCGTGGAGGAGCTAGCCAACGTCCTTTGACCCCAAATCAGAACCAGCAGGGACAACAAACAGATCCCCTTGTAGCAGCTGCAGCAGTGAATTCTGCTCTTGCCTTTGGACAAGGATTGGCTGCAGGTATGCCAGGTTATCCAGTCTTGGCACCTGCTGCTTACTATGACCAAACTGGTGCCCTTGTGGTGAATGCAGGGGCAAGAAATGGCCTCGGAGCTCCAGTCCGGCTTGTTGCTCCAGCCCCAGTCATCATTAGCTCCTCAGCTGCACAAGCAGCTGTTGCAGCGGCTGCAGCGTCAGCAAACGGAGCAGCTGGTGGTCTTGCCGGAACTACAAATGGACCATTTCGACCTTTAGGCACCCAGCAGCCCCAGCCCCAGCCTCAGCAGCAGCCCAGTAACAATCTGGCTTCCAGCTCTTTCTATGGCAACAACTCTCTGAGCAGTAACTCCCAGAGCAGCTCCCTCTTCTCTCAGGGCTCTGCACAGCCTGCCAACACGTCCTTGGGATTCGGAAGCAGCAGTTCCCTTGGTGCCACCCTGGGATCGGCCCTTGGAGGGTTTGGAACAGCAGTTGCAAACTCCAACACTGGCAGTGGCTCCCGCCGTGACTCCCTGACTGGCAGCAGTGACCTTTATAAGAGGACATCGAGCAGCTTGGCCCCCATTGGACACAGTTTTTATAGCAGCCTTAGCTATTCCTCCTCTCCTGGACCCGTGGGCATGCCTCTCCCTAGTCAGGGACCAGGACATTCACAGACACCACCTCCTTCCCTCTCTTCACATGGATCCTCTTCAAGCTTAAACCTGGGAGGACTCACAAACGGCAGTGGAAGATACATCTCTGCTGCTCCCGGTGCCGAAGCCAAGTACCGCAGTGCAAGCAGTGCCTCCAGCCTTTTCAGCCCCAGCAGCACCCTTTTTTCTTCCTCTCGTTTGCGATATGGAATGTCTGATGTTATGCCCTCTGGCAGGAGCAGACTTCTGGAAGATTTTCGAAACAACCGGTACCCTAATTTACAACTGCGGGAGATTGCTGGGCATATAATGGAATTTTCTCAAGACCAGCATGGCTCTAGATTCATTCAGCTGAAACTAGAGCGTGCCACAGCAGCTGAGCGCCAGCTTGTCTTCAATGAAATCCTCCAGGCTGCGTACCAACTTATGGTGGATGTGTTTGGAAATTACGTCATTCAGAAGTTTTTTGAATTTGGCAGTCATGAACAGAAGCTGGCTCTGGCTGAACGGATTCGAGGCCATGTCCTATCATTGGCACTGCAGATGTACGGCTGCCGAGTCATCCAGAAAGCTTTGGAGTTTATTCCCTCAGACCAGCAGGTAATTAACGAGATGGTGCGGGAGCTAGATGGCCATGTCCTGAAGTGTGTGAAAGACCAGAACGGCAACCATGTGGTTCAGAAGTGCATTGAGTGTGTACAGCCCCAGTCTTTGCAGTTTATCATCGATGCATTCAAGGGGCAGGTGTTTGCTTTGTCTACGCACCCGTACGGCTGTAGGGTGATCCAGAGGATCCTAGAGCACTGTCTCCCTGACCAGACCCTCCCCATCCTGGAGGAGCTTCACCAGCACACAGAGCAGCTTGTGCAGGATCAGTATGGAAATTATGTAATCCAACATGTACTGGAGCATGGTCGTCCTGAGGATAAAAGCAAAATTGTAGCAGAGATCCGAGGCAATGTACTTGTATTGAGTCAGCACAAGTTTGCAAGCAATGTTGTGGAGAAGTGTGTTACTCACGCCTCACGTACAGAGCGTGCTGTGCTCATTGATGAGGTGTGCACCATGAACGACGGTCCCCACAGTGCCTTATACACCATGATGAAGGATCAGTATGCTAACTATGTGGTCCAGAAGATGATCGATGTGGCTGAGCCGGGTCAGCGGAAGATTGTCATGCACAAGATCCGGCCACACATTGCGACGCTTCGAAAGTACACCTATGGCAAGCACATCCTGGCCAAGCTCGAGAAGTACTACATGAAGAATGGCGTGGACTTGGGGCCCATTTGTGGTCCCCCTAATGGTATCATCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSI
GVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNTSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGEQLLPGKKFWETDESSKDGPKGIFLG
DQWRDSAWGTSDHSVSQPIMVQRRPGQSFHVNSEVNSVLSPRSESGGLGVSMVEYVLSSSPGDSCLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDL
KEEGDVMDKTNGLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPNQNGSEGLAQLTSTNGAKPVEDFSNMESQSVPLDPMEHVGMEPLQFDYSGTQVPV
DSAAATVGLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIAGLAPAAFVPNPYIISAAPPGTDPYTAGLAAAATLGPAVVPHQYYGVTPWGV
YPASLFQQQAAAAAAATNSATQQSAPQAQQGQQQVLRGGASQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYDQTGALVVN
AGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQQPQPQPQQQPSNNLASSSFYGNNSLSSNSQSSSLFSQGSAQP
ANTSLGFGSSSSLGATLGSALGGFGTAVANSNTGSGSRRDSLTGSSDLYKRTSSSLAPIGHSFYSSLSYSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGS
SSSLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFIQL
KLERATAAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGSHEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQVINEMVRELDGHVLKCVK
DQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIR
GNVLVLSQHKFASNVVEKCVTHASRTERAVLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYTYGKHILAKLEK
YYMKNGVDLGPICGPPNGII*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU53601
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee